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In silico analyses and global transcriptional profiling reveal novel putative targets for Pea3 transcription factor related to its function in neurons
Pea3 transcription factor belongs to the PEA3 subfamily within the ETS domain transcription factor superfamily, and has been largely studied in relation to its role in breast cancer metastasis. Nonetheless, Pea3 plays a role not only in breast tumor, but also in other tissues with branching morphoge...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5291419/ https://www.ncbi.nlm.nih.gov/pubmed/28158215 http://dx.doi.org/10.1371/journal.pone.0170585 |
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author | Kandemir, Başak Dag, Ugur Bakir Gungor, Burcu Durasi, İlknur Melis Erdogan, Burcu Sahin, Eray Sezerman, Ugur Aksan Kurnaz, Isil |
author_facet | Kandemir, Başak Dag, Ugur Bakir Gungor, Burcu Durasi, İlknur Melis Erdogan, Burcu Sahin, Eray Sezerman, Ugur Aksan Kurnaz, Isil |
author_sort | Kandemir, Başak |
collection | PubMed |
description | Pea3 transcription factor belongs to the PEA3 subfamily within the ETS domain transcription factor superfamily, and has been largely studied in relation to its role in breast cancer metastasis. Nonetheless, Pea3 plays a role not only in breast tumor, but also in other tissues with branching morphogenesis, including kidneys, blood vasculature, bronchi and the developing nervous system. Identification of Pea3 target promoters in these systems are important for a thorough understanding of how Pea3 functions. Present study particularly focuses on the identification of novel neuronal targets of Pea3 in a combinatorial approach, through curation, computational analysis and microarray studies in a neuronal model system, SH-SY5Y neuroblastoma cells. We not only show that quite a number of genes in cancer, immune system and cell cycle pathways, among many others, are either up- or down-regulated by Pea3, but also identify novel targets including ephrins and ephrin receptors, semaphorins, cell adhesion molecules, as well as metalloproteases such as kallikreins, to be among potential target promoters in neuronal systems. Our overall results indicate that rather than early stages of neurite extension and axonal guidance, Pea3 is more involved in target identification and synaptic maturation. |
format | Online Article Text |
id | pubmed-5291419 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-52914192017-02-17 In silico analyses and global transcriptional profiling reveal novel putative targets for Pea3 transcription factor related to its function in neurons Kandemir, Başak Dag, Ugur Bakir Gungor, Burcu Durasi, İlknur Melis Erdogan, Burcu Sahin, Eray Sezerman, Ugur Aksan Kurnaz, Isil PLoS One Research Article Pea3 transcription factor belongs to the PEA3 subfamily within the ETS domain transcription factor superfamily, and has been largely studied in relation to its role in breast cancer metastasis. Nonetheless, Pea3 plays a role not only in breast tumor, but also in other tissues with branching morphogenesis, including kidneys, blood vasculature, bronchi and the developing nervous system. Identification of Pea3 target promoters in these systems are important for a thorough understanding of how Pea3 functions. Present study particularly focuses on the identification of novel neuronal targets of Pea3 in a combinatorial approach, through curation, computational analysis and microarray studies in a neuronal model system, SH-SY5Y neuroblastoma cells. We not only show that quite a number of genes in cancer, immune system and cell cycle pathways, among many others, are either up- or down-regulated by Pea3, but also identify novel targets including ephrins and ephrin receptors, semaphorins, cell adhesion molecules, as well as metalloproteases such as kallikreins, to be among potential target promoters in neuronal systems. Our overall results indicate that rather than early stages of neurite extension and axonal guidance, Pea3 is more involved in target identification and synaptic maturation. Public Library of Science 2017-02-03 /pmc/articles/PMC5291419/ /pubmed/28158215 http://dx.doi.org/10.1371/journal.pone.0170585 Text en © 2017 Kandemir et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Kandemir, Başak Dag, Ugur Bakir Gungor, Burcu Durasi, İlknur Melis Erdogan, Burcu Sahin, Eray Sezerman, Ugur Aksan Kurnaz, Isil In silico analyses and global transcriptional profiling reveal novel putative targets for Pea3 transcription factor related to its function in neurons |
title | In silico analyses and global transcriptional profiling reveal novel putative targets for Pea3 transcription factor related to its function in neurons |
title_full | In silico analyses and global transcriptional profiling reveal novel putative targets for Pea3 transcription factor related to its function in neurons |
title_fullStr | In silico analyses and global transcriptional profiling reveal novel putative targets for Pea3 transcription factor related to its function in neurons |
title_full_unstemmed | In silico analyses and global transcriptional profiling reveal novel putative targets for Pea3 transcription factor related to its function in neurons |
title_short | In silico analyses and global transcriptional profiling reveal novel putative targets for Pea3 transcription factor related to its function in neurons |
title_sort | in silico analyses and global transcriptional profiling reveal novel putative targets for pea3 transcription factor related to its function in neurons |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5291419/ https://www.ncbi.nlm.nih.gov/pubmed/28158215 http://dx.doi.org/10.1371/journal.pone.0170585 |
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