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Preparation and immunogenicity of influenza virus-like particles using nitrocellulose membrane filtration

PURPOSE: Nitrocellulose membrane–based filtration system (NCFS) is widely used for protein concentration. In this study, we applied NCFS for production of virus-like particle (VLP) as a vaccine candidate and evaluated yield property and immunogenicity. MATERIALS AND METHODS: Influenza VLPs were gene...

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Autores principales: Park, Young Chan, Song, Jae Min
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Vaccine Society 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5292359/
https://www.ncbi.nlm.nih.gov/pubmed/28168175
http://dx.doi.org/10.7774/cevr.2017.6.1.61
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author Park, Young Chan
Song, Jae Min
author_facet Park, Young Chan
Song, Jae Min
author_sort Park, Young Chan
collection PubMed
description PURPOSE: Nitrocellulose membrane–based filtration system (NCFS) is widely used for protein concentration. In this study, we applied NCFS for production of virus-like particle (VLP) as a vaccine candidate and evaluated yield property and immunogenicity. MATERIALS AND METHODS: Influenza VLPs were generated by baculovirus-insect cell protein expression system. NCFS and sucrose gradient ultracentrifugation were used for purification of VLP. Immunogenicity of VLP was evaluated by animal experiment. RESULTS: Influenza VLPs expressing hemagglutinin (HA) and neuraminidase proteins derived from highly pathogenic influenza virus (H5N8) were effectively produced and purified by NCFS. HA activity of VLP which correlated with antigenicity was well conserved during multiple purification steps. This NCFS based purified VLPs induced influenza virus–specific antibody responses. CONCLUSION: Our results indicate that the influenza VLP vaccine could be prepared by NCFS without loss of immunogenicity and elicit antigen-specific immune responses.
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spelling pubmed-52923592017-02-06 Preparation and immunogenicity of influenza virus-like particles using nitrocellulose membrane filtration Park, Young Chan Song, Jae Min Clin Exp Vaccine Res Original Article PURPOSE: Nitrocellulose membrane–based filtration system (NCFS) is widely used for protein concentration. In this study, we applied NCFS for production of virus-like particle (VLP) as a vaccine candidate and evaluated yield property and immunogenicity. MATERIALS AND METHODS: Influenza VLPs were generated by baculovirus-insect cell protein expression system. NCFS and sucrose gradient ultracentrifugation were used for purification of VLP. Immunogenicity of VLP was evaluated by animal experiment. RESULTS: Influenza VLPs expressing hemagglutinin (HA) and neuraminidase proteins derived from highly pathogenic influenza virus (H5N8) were effectively produced and purified by NCFS. HA activity of VLP which correlated with antigenicity was well conserved during multiple purification steps. This NCFS based purified VLPs induced influenza virus–specific antibody responses. CONCLUSION: Our results indicate that the influenza VLP vaccine could be prepared by NCFS without loss of immunogenicity and elicit antigen-specific immune responses. The Korean Vaccine Society 2017-01 2017-01-25 /pmc/articles/PMC5292359/ /pubmed/28168175 http://dx.doi.org/10.7774/cevr.2017.6.1.61 Text en © Korean Vaccine Society. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Park, Young Chan
Song, Jae Min
Preparation and immunogenicity of influenza virus-like particles using nitrocellulose membrane filtration
title Preparation and immunogenicity of influenza virus-like particles using nitrocellulose membrane filtration
title_full Preparation and immunogenicity of influenza virus-like particles using nitrocellulose membrane filtration
title_fullStr Preparation and immunogenicity of influenza virus-like particles using nitrocellulose membrane filtration
title_full_unstemmed Preparation and immunogenicity of influenza virus-like particles using nitrocellulose membrane filtration
title_short Preparation and immunogenicity of influenza virus-like particles using nitrocellulose membrane filtration
title_sort preparation and immunogenicity of influenza virus-like particles using nitrocellulose membrane filtration
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5292359/
https://www.ncbi.nlm.nih.gov/pubmed/28168175
http://dx.doi.org/10.7774/cevr.2017.6.1.61
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