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Transcriptional Profile of Kidney from Type 2 Diabetic db/db Mice

Diabetic nephropathy (DN), a common diabetic microvascular complication, is characterized by progressive glomerular sclerosis and tubulointerstitial fibrosis. However, the underlying mechanisms involved in DN remain to be elucidated. We explored changes in the transcriptional profile in spontaneous...

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Autores principales: Zhang, Haojun, Zhao, Tingting, Li, Zhiguo, Yan, Meihua, Zhao, Hailing, Zhu, Bin, Li, Ping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5292381/
https://www.ncbi.nlm.nih.gov/pubmed/28232950
http://dx.doi.org/10.1155/2017/8391253
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author Zhang, Haojun
Zhao, Tingting
Li, Zhiguo
Yan, Meihua
Zhao, Hailing
Zhu, Bin
Li, Ping
author_facet Zhang, Haojun
Zhao, Tingting
Li, Zhiguo
Yan, Meihua
Zhao, Hailing
Zhu, Bin
Li, Ping
author_sort Zhang, Haojun
collection PubMed
description Diabetic nephropathy (DN), a common diabetic microvascular complication, is characterized by progressive glomerular sclerosis and tubulointerstitial fibrosis. However, the underlying mechanisms involved in DN remain to be elucidated. We explored changes in the transcriptional profile in spontaneous type 2 diabetic db/db mice by using the cDNA microarray. Compared with control db/m mice, the db/db mice exhibited marked increases in body weight, kidney weight, and urinary albumin excretion. Renal histological analysis revealed mesangial expansion and thickness of the basement membrane in the kidney of the db/db mice. A total of 355 differentially expressed genes (DEGs) were identified by microarray analysis. Pathway enrichment analysis suggested that biological oxidation, bile acid metabolism, and steroid hormone synthesis were the 3 major significant pathways. The top 10 hub genes were selected from the constructed PPI network of DEGs, including Ccnb2 and Nr1i2, which remained largely unclear in DN. We believe that our study can help elucidate the molecular mechanisms underlying DN.
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spelling pubmed-52923812017-02-23 Transcriptional Profile of Kidney from Type 2 Diabetic db/db Mice Zhang, Haojun Zhao, Tingting Li, Zhiguo Yan, Meihua Zhao, Hailing Zhu, Bin Li, Ping J Diabetes Res Research Article Diabetic nephropathy (DN), a common diabetic microvascular complication, is characterized by progressive glomerular sclerosis and tubulointerstitial fibrosis. However, the underlying mechanisms involved in DN remain to be elucidated. We explored changes in the transcriptional profile in spontaneous type 2 diabetic db/db mice by using the cDNA microarray. Compared with control db/m mice, the db/db mice exhibited marked increases in body weight, kidney weight, and urinary albumin excretion. Renal histological analysis revealed mesangial expansion and thickness of the basement membrane in the kidney of the db/db mice. A total of 355 differentially expressed genes (DEGs) were identified by microarray analysis. Pathway enrichment analysis suggested that biological oxidation, bile acid metabolism, and steroid hormone synthesis were the 3 major significant pathways. The top 10 hub genes were selected from the constructed PPI network of DEGs, including Ccnb2 and Nr1i2, which remained largely unclear in DN. We believe that our study can help elucidate the molecular mechanisms underlying DN. Hindawi Publishing Corporation 2017 2017-01-23 /pmc/articles/PMC5292381/ /pubmed/28232950 http://dx.doi.org/10.1155/2017/8391253 Text en Copyright © 2017 Haojun Zhang et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Zhang, Haojun
Zhao, Tingting
Li, Zhiguo
Yan, Meihua
Zhao, Hailing
Zhu, Bin
Li, Ping
Transcriptional Profile of Kidney from Type 2 Diabetic db/db Mice
title Transcriptional Profile of Kidney from Type 2 Diabetic db/db Mice
title_full Transcriptional Profile of Kidney from Type 2 Diabetic db/db Mice
title_fullStr Transcriptional Profile of Kidney from Type 2 Diabetic db/db Mice
title_full_unstemmed Transcriptional Profile of Kidney from Type 2 Diabetic db/db Mice
title_short Transcriptional Profile of Kidney from Type 2 Diabetic db/db Mice
title_sort transcriptional profile of kidney from type 2 diabetic db/db mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5292381/
https://www.ncbi.nlm.nih.gov/pubmed/28232950
http://dx.doi.org/10.1155/2017/8391253
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