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Raman Spectroscopic Analyses of Jaw Periosteal Cell Mineralization
To achieve safer patient treatments, serum-free cell culture conditions have to be established for cell therapies. In previous studies, we demonstrated that serum-free culture favored the proliferation of MSCA-1(+) osteoprogenitors derived from the jaw periosteum. In this study, the in vitro formati...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5292402/ https://www.ncbi.nlm.nih.gov/pubmed/28232849 http://dx.doi.org/10.1155/2017/1651376 |
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author | Brauchle, Eva Carvajal Berrio, Daniel Rieger, Melanie Schenke-Layland, Katja Reinert, Siegmar Alexander, Dorothea |
author_facet | Brauchle, Eva Carvajal Berrio, Daniel Rieger, Melanie Schenke-Layland, Katja Reinert, Siegmar Alexander, Dorothea |
author_sort | Brauchle, Eva |
collection | PubMed |
description | To achieve safer patient treatments, serum-free cell culture conditions have to be established for cell therapies. In previous studies, we demonstrated that serum-free culture favored the proliferation of MSCA-1(+) osteoprogenitors derived from the jaw periosteum. In this study, the in vitro formation of bone-specific matrix by MSCA-1(+) jaw periosteal cells (JPCs, 3 donors) was assessed and compared under serum-free and serum-containing media conditions using the marker-free Raman spectroscopy. Based on a standard fluorescence assay, JPCs from one patient were not able to mineralize under serum-containing culture conditions, whereas the other cells showed similar mineralization levels under both conditions. Raman spectra from mineralizing MSCA-1(+) JPCs revealed higher levels of hydroxyapatite formation and higher mineral to matrix ratios under serum-free culture conditions. Higher carbonate to phosphate ratios and higher crystallinity in JPCs cultured under serum-containing conditions indicated immature bone formation. Due to reduced collagen production under serum-free conditions, we obtained significant differences in collagen maturity and proline to hydroxyproline ratios compared to serum-free conditions. We conclude that Raman spectroscopy is a useful tool for the assessment and noninvasive monitoring of in vitro mineralization of osteoprogenitor cells. Further studies should extend this knowledge and improve JPC mineralization by optimizing culture conditions. |
format | Online Article Text |
id | pubmed-5292402 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-52924022017-02-23 Raman Spectroscopic Analyses of Jaw Periosteal Cell Mineralization Brauchle, Eva Carvajal Berrio, Daniel Rieger, Melanie Schenke-Layland, Katja Reinert, Siegmar Alexander, Dorothea Stem Cells Int Research Article To achieve safer patient treatments, serum-free cell culture conditions have to be established for cell therapies. In previous studies, we demonstrated that serum-free culture favored the proliferation of MSCA-1(+) osteoprogenitors derived from the jaw periosteum. In this study, the in vitro formation of bone-specific matrix by MSCA-1(+) jaw periosteal cells (JPCs, 3 donors) was assessed and compared under serum-free and serum-containing media conditions using the marker-free Raman spectroscopy. Based on a standard fluorescence assay, JPCs from one patient were not able to mineralize under serum-containing culture conditions, whereas the other cells showed similar mineralization levels under both conditions. Raman spectra from mineralizing MSCA-1(+) JPCs revealed higher levels of hydroxyapatite formation and higher mineral to matrix ratios under serum-free culture conditions. Higher carbonate to phosphate ratios and higher crystallinity in JPCs cultured under serum-containing conditions indicated immature bone formation. Due to reduced collagen production under serum-free conditions, we obtained significant differences in collagen maturity and proline to hydroxyproline ratios compared to serum-free conditions. We conclude that Raman spectroscopy is a useful tool for the assessment and noninvasive monitoring of in vitro mineralization of osteoprogenitor cells. Further studies should extend this knowledge and improve JPC mineralization by optimizing culture conditions. Hindawi Publishing Corporation 2017 2017-01-23 /pmc/articles/PMC5292402/ /pubmed/28232849 http://dx.doi.org/10.1155/2017/1651376 Text en Copyright © 2017 Eva Brauchle et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Brauchle, Eva Carvajal Berrio, Daniel Rieger, Melanie Schenke-Layland, Katja Reinert, Siegmar Alexander, Dorothea Raman Spectroscopic Analyses of Jaw Periosteal Cell Mineralization |
title | Raman Spectroscopic Analyses of Jaw Periosteal Cell Mineralization |
title_full | Raman Spectroscopic Analyses of Jaw Periosteal Cell Mineralization |
title_fullStr | Raman Spectroscopic Analyses of Jaw Periosteal Cell Mineralization |
title_full_unstemmed | Raman Spectroscopic Analyses of Jaw Periosteal Cell Mineralization |
title_short | Raman Spectroscopic Analyses of Jaw Periosteal Cell Mineralization |
title_sort | raman spectroscopic analyses of jaw periosteal cell mineralization |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5292402/ https://www.ncbi.nlm.nih.gov/pubmed/28232849 http://dx.doi.org/10.1155/2017/1651376 |
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