Cargando…
Quantitative global proteome and lysine succinylome analyses provide insights into metabolic regulation and lymph node metastasis in gastric cancer
With the rapid development of high-throughput quantitative proteomic and transcriptomic approaches, the molecular mechanisms of cancers have been comprehensively explored. However, cancer is a multi-dimensional disease with sophisticated regulations, and few studies focus on the crosstalk among mult...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5292683/ https://www.ncbi.nlm.nih.gov/pubmed/28165029 http://dx.doi.org/10.1038/srep42053 |
_version_ | 1782504969971171328 |
---|---|
author | Song, Yongxi Wang, Jun Cheng, Zhongyi Gao, Peng Sun, Jingxu Chen, Xiaowan Chen, Chen Wang, Yunlong Wang, Zhenning |
author_facet | Song, Yongxi Wang, Jun Cheng, Zhongyi Gao, Peng Sun, Jingxu Chen, Xiaowan Chen, Chen Wang, Yunlong Wang, Zhenning |
author_sort | Song, Yongxi |
collection | PubMed |
description | With the rapid development of high-throughput quantitative proteomic and transcriptomic approaches, the molecular mechanisms of cancers have been comprehensively explored. However, cancer is a multi-dimensional disease with sophisticated regulations, and few studies focus on the crosstalk among multiomics. In order to explore the molecular mechanisms of gastric cancer (GC), particularly in the process of lymph node metastasis (LNM), we investigated dynamic profiling changes as well as crosstalk between long non-coding RNAs (lncRNAs), the proteome, and the lysine succinylome. Our study reports the first qualitative and quantitative profile of lysine succinylation in GC. We identified a novel mechanism through which the TCA cycle and pentose phosphate pathway might be regulated through lysine succinylation in their core enzymes. We then examined the potential of using lysine succinylation as a biomarker for GC and successfully developed a succinylation-dependent antibody for the K569 site in Caldesmon as putative biomarker. Finally, we investigated the relationship between the lysine succinylome and lncRNAs, identifying potential crosstalks between two lncRNAs and one succinylation site. These results expand our understanding of the mechanisms of tumorigenesis and provide new information for the diagnosis and prognosis of GC. |
format | Online Article Text |
id | pubmed-5292683 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-52926832017-02-10 Quantitative global proteome and lysine succinylome analyses provide insights into metabolic regulation and lymph node metastasis in gastric cancer Song, Yongxi Wang, Jun Cheng, Zhongyi Gao, Peng Sun, Jingxu Chen, Xiaowan Chen, Chen Wang, Yunlong Wang, Zhenning Sci Rep Article With the rapid development of high-throughput quantitative proteomic and transcriptomic approaches, the molecular mechanisms of cancers have been comprehensively explored. However, cancer is a multi-dimensional disease with sophisticated regulations, and few studies focus on the crosstalk among multiomics. In order to explore the molecular mechanisms of gastric cancer (GC), particularly in the process of lymph node metastasis (LNM), we investigated dynamic profiling changes as well as crosstalk between long non-coding RNAs (lncRNAs), the proteome, and the lysine succinylome. Our study reports the first qualitative and quantitative profile of lysine succinylation in GC. We identified a novel mechanism through which the TCA cycle and pentose phosphate pathway might be regulated through lysine succinylation in their core enzymes. We then examined the potential of using lysine succinylation as a biomarker for GC and successfully developed a succinylation-dependent antibody for the K569 site in Caldesmon as putative biomarker. Finally, we investigated the relationship between the lysine succinylome and lncRNAs, identifying potential crosstalks between two lncRNAs and one succinylation site. These results expand our understanding of the mechanisms of tumorigenesis and provide new information for the diagnosis and prognosis of GC. Nature Publishing Group 2017-02-06 /pmc/articles/PMC5292683/ /pubmed/28165029 http://dx.doi.org/10.1038/srep42053 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Song, Yongxi Wang, Jun Cheng, Zhongyi Gao, Peng Sun, Jingxu Chen, Xiaowan Chen, Chen Wang, Yunlong Wang, Zhenning Quantitative global proteome and lysine succinylome analyses provide insights into metabolic regulation and lymph node metastasis in gastric cancer |
title | Quantitative global proteome and lysine succinylome analyses provide insights into metabolic regulation and lymph node metastasis in gastric cancer |
title_full | Quantitative global proteome and lysine succinylome analyses provide insights into metabolic regulation and lymph node metastasis in gastric cancer |
title_fullStr | Quantitative global proteome and lysine succinylome analyses provide insights into metabolic regulation and lymph node metastasis in gastric cancer |
title_full_unstemmed | Quantitative global proteome and lysine succinylome analyses provide insights into metabolic regulation and lymph node metastasis in gastric cancer |
title_short | Quantitative global proteome and lysine succinylome analyses provide insights into metabolic regulation and lymph node metastasis in gastric cancer |
title_sort | quantitative global proteome and lysine succinylome analyses provide insights into metabolic regulation and lymph node metastasis in gastric cancer |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5292683/ https://www.ncbi.nlm.nih.gov/pubmed/28165029 http://dx.doi.org/10.1038/srep42053 |
work_keys_str_mv | AT songyongxi quantitativeglobalproteomeandlysinesuccinylomeanalysesprovideinsightsintometabolicregulationandlymphnodemetastasisingastriccancer AT wangjun quantitativeglobalproteomeandlysinesuccinylomeanalysesprovideinsightsintometabolicregulationandlymphnodemetastasisingastriccancer AT chengzhongyi quantitativeglobalproteomeandlysinesuccinylomeanalysesprovideinsightsintometabolicregulationandlymphnodemetastasisingastriccancer AT gaopeng quantitativeglobalproteomeandlysinesuccinylomeanalysesprovideinsightsintometabolicregulationandlymphnodemetastasisingastriccancer AT sunjingxu quantitativeglobalproteomeandlysinesuccinylomeanalysesprovideinsightsintometabolicregulationandlymphnodemetastasisingastriccancer AT chenxiaowan quantitativeglobalproteomeandlysinesuccinylomeanalysesprovideinsightsintometabolicregulationandlymphnodemetastasisingastriccancer AT chenchen quantitativeglobalproteomeandlysinesuccinylomeanalysesprovideinsightsintometabolicregulationandlymphnodemetastasisingastriccancer AT wangyunlong quantitativeglobalproteomeandlysinesuccinylomeanalysesprovideinsightsintometabolicregulationandlymphnodemetastasisingastriccancer AT wangzhenning quantitativeglobalproteomeandlysinesuccinylomeanalysesprovideinsightsintometabolicregulationandlymphnodemetastasisingastriccancer |