A novel monoclonal antibody against the von Willebrand Factor A2 domain reduces its cleavage by ADAMTS13

We developed a novel murine monoclonal antibody (mAb) against the C-terminal α-helix of the human von Willebrand factor A2, designated SZ-179. We showed that SZ-179 inhibited the interactions between VWF and ADAMTS13 and prevented the degradation of high molecular weight VWF multimers. Importantly,...

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Detalles Bibliográficos
Autores principales: Zhang, Lulu, Su, Jian, Shen, Fei, Ma, Zhenni, Zhao, Yiming, Xia, Lijun, Ruan, Changgeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Letter to the Editor
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5292787/
https://www.ncbi.nlm.nih.gov/pubmed/28166803
http://dx.doi.org/10.1186/s13045-017-0407-1
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author Zhang, Lulu
Su, Jian
Shen, Fei
Ma, Zhenni
Zhao, Yiming
Xia, Lijun
Ruan, Changgeng
author_facet Zhang, Lulu
Su, Jian
Shen, Fei
Ma, Zhenni
Zhao, Yiming
Xia, Lijun
Ruan, Changgeng
author_sort Zhang, Lulu
collection PubMed
description We developed a novel murine monoclonal antibody (mAb) against the C-terminal α-helix of the human von Willebrand factor A2, designated SZ-179. We showed that SZ-179 inhibited the interactions between VWF and ADAMTS13 and prevented the degradation of high molecular weight VWF multimers. Importantly, SZ-179 reduced the proteolysis of VWF-R1597W mutant by rADAMTS13 dose-dependently under native conditions. Our findings reveal a potential therapeutic target for bleeding disorders. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13045-017-0407-1) contains supplementary material, which is available to authorized users.
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spelling pubmed-52927872017-02-10 A novel monoclonal antibody against the von Willebrand Factor A2 domain reduces its cleavage by ADAMTS13 Zhang, Lulu Su, Jian Shen, Fei Ma, Zhenni Zhao, Yiming Xia, Lijun Ruan, Changgeng J Hematol Oncol Letter to the Editor We developed a novel murine monoclonal antibody (mAb) against the C-terminal α-helix of the human von Willebrand factor A2, designated SZ-179. We showed that SZ-179 inhibited the interactions between VWF and ADAMTS13 and prevented the degradation of high molecular weight VWF multimers. Importantly, SZ-179 reduced the proteolysis of VWF-R1597W mutant by rADAMTS13 dose-dependently under native conditions. Our findings reveal a potential therapeutic target for bleeding disorders. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13045-017-0407-1) contains supplementary material, which is available to authorized users. BioMed Central 2017-02-06 /pmc/articles/PMC5292787/ /pubmed/28166803 http://dx.doi.org/10.1186/s13045-017-0407-1 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Letter to the Editor
Zhang, Lulu
Su, Jian
Shen, Fei
Ma, Zhenni
Zhao, Yiming
Xia, Lijun
Ruan, Changgeng
A novel monoclonal antibody against the von Willebrand Factor A2 domain reduces its cleavage by ADAMTS13
title A novel monoclonal antibody against the von Willebrand Factor A2 domain reduces its cleavage by ADAMTS13
title_full A novel monoclonal antibody against the von Willebrand Factor A2 domain reduces its cleavage by ADAMTS13
title_fullStr A novel monoclonal antibody against the von Willebrand Factor A2 domain reduces its cleavage by ADAMTS13
title_full_unstemmed A novel monoclonal antibody against the von Willebrand Factor A2 domain reduces its cleavage by ADAMTS13
title_short A novel monoclonal antibody against the von Willebrand Factor A2 domain reduces its cleavage by ADAMTS13
title_sort novel monoclonal antibody against the von willebrand factor a2 domain reduces its cleavage by adamts13
topic Letter to the Editor
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5292787/
https://www.ncbi.nlm.nih.gov/pubmed/28166803
http://dx.doi.org/10.1186/s13045-017-0407-1
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