Cargando…

Small RNA-directed epigenetic programming of embryonic stem cell cardiac differentiation

Microinjection of small noncoding RNAs in one-cell embryos was reported in several instances to result in transcriptional activation of target genes. To determine the molecular mechanisms involved and to explore whether such epigenetic regulations could play a role in early development, we used a ce...

Descripción completa

Detalles Bibliográficos
Autores principales: Ghanbarian, Hossein, Wagner, Nicole, Michiels, Jean-François, Cuzin, François, Wagner, Kay-Dietrich, Rassoulzadegan, Minoo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5292948/
https://www.ncbi.nlm.nih.gov/pubmed/28165496
http://dx.doi.org/10.1038/srep41799
_version_ 1782505016679989248
author Ghanbarian, Hossein
Wagner, Nicole
Michiels, Jean-François
Cuzin, François
Wagner, Kay-Dietrich
Rassoulzadegan, Minoo
author_facet Ghanbarian, Hossein
Wagner, Nicole
Michiels, Jean-François
Cuzin, François
Wagner, Kay-Dietrich
Rassoulzadegan, Minoo
author_sort Ghanbarian, Hossein
collection PubMed
description Microinjection of small noncoding RNAs in one-cell embryos was reported in several instances to result in transcriptional activation of target genes. To determine the molecular mechanisms involved and to explore whether such epigenetic regulations could play a role in early development, we used a cell culture system as close as possible to the embryonic state. We report efficient cardiac differentiation of embryonic stem (ES) cells induced by small non-coding RNAs with sequences of Cdk9, a key player in cardiomyocyte differentiation. Transfer of oligoribonucleotides representing parts of the Cdk9 mRNA into ES and mouse embryo fibroblast cultures resulted in upregulation of transcription. Dependency on Argonaute proteins and endogenous antisense transcripts indicated that the inducer oligoribonucleotides were processed by the RNAi machinery. Upregulation of Cdk9 expression resulted in increased efficiency of cardiac differentiation suggesting a potential tool for stem cell-based regenerative medicine.
format Online
Article
Text
id pubmed-5292948
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-52929482017-02-10 Small RNA-directed epigenetic programming of embryonic stem cell cardiac differentiation Ghanbarian, Hossein Wagner, Nicole Michiels, Jean-François Cuzin, François Wagner, Kay-Dietrich Rassoulzadegan, Minoo Sci Rep Article Microinjection of small noncoding RNAs in one-cell embryos was reported in several instances to result in transcriptional activation of target genes. To determine the molecular mechanisms involved and to explore whether such epigenetic regulations could play a role in early development, we used a cell culture system as close as possible to the embryonic state. We report efficient cardiac differentiation of embryonic stem (ES) cells induced by small non-coding RNAs with sequences of Cdk9, a key player in cardiomyocyte differentiation. Transfer of oligoribonucleotides representing parts of the Cdk9 mRNA into ES and mouse embryo fibroblast cultures resulted in upregulation of transcription. Dependency on Argonaute proteins and endogenous antisense transcripts indicated that the inducer oligoribonucleotides were processed by the RNAi machinery. Upregulation of Cdk9 expression resulted in increased efficiency of cardiac differentiation suggesting a potential tool for stem cell-based regenerative medicine. Nature Publishing Group 2017-02-06 /pmc/articles/PMC5292948/ /pubmed/28165496 http://dx.doi.org/10.1038/srep41799 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Ghanbarian, Hossein
Wagner, Nicole
Michiels, Jean-François
Cuzin, François
Wagner, Kay-Dietrich
Rassoulzadegan, Minoo
Small RNA-directed epigenetic programming of embryonic stem cell cardiac differentiation
title Small RNA-directed epigenetic programming of embryonic stem cell cardiac differentiation
title_full Small RNA-directed epigenetic programming of embryonic stem cell cardiac differentiation
title_fullStr Small RNA-directed epigenetic programming of embryonic stem cell cardiac differentiation
title_full_unstemmed Small RNA-directed epigenetic programming of embryonic stem cell cardiac differentiation
title_short Small RNA-directed epigenetic programming of embryonic stem cell cardiac differentiation
title_sort small rna-directed epigenetic programming of embryonic stem cell cardiac differentiation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5292948/
https://www.ncbi.nlm.nih.gov/pubmed/28165496
http://dx.doi.org/10.1038/srep41799
work_keys_str_mv AT ghanbarianhossein smallrnadirectedepigeneticprogrammingofembryonicstemcellcardiacdifferentiation
AT wagnernicole smallrnadirectedepigeneticprogrammingofembryonicstemcellcardiacdifferentiation
AT michielsjeanfrancois smallrnadirectedepigeneticprogrammingofembryonicstemcellcardiacdifferentiation
AT cuzinfrancois smallrnadirectedepigeneticprogrammingofembryonicstemcellcardiacdifferentiation
AT wagnerkaydietrich smallrnadirectedepigeneticprogrammingofembryonicstemcellcardiacdifferentiation
AT rassoulzadeganminoo smallrnadirectedepigeneticprogrammingofembryonicstemcellcardiacdifferentiation