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Small RNA-directed epigenetic programming of embryonic stem cell cardiac differentiation
Microinjection of small noncoding RNAs in one-cell embryos was reported in several instances to result in transcriptional activation of target genes. To determine the molecular mechanisms involved and to explore whether such epigenetic regulations could play a role in early development, we used a ce...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5292948/ https://www.ncbi.nlm.nih.gov/pubmed/28165496 http://dx.doi.org/10.1038/srep41799 |
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author | Ghanbarian, Hossein Wagner, Nicole Michiels, Jean-François Cuzin, François Wagner, Kay-Dietrich Rassoulzadegan, Minoo |
author_facet | Ghanbarian, Hossein Wagner, Nicole Michiels, Jean-François Cuzin, François Wagner, Kay-Dietrich Rassoulzadegan, Minoo |
author_sort | Ghanbarian, Hossein |
collection | PubMed |
description | Microinjection of small noncoding RNAs in one-cell embryos was reported in several instances to result in transcriptional activation of target genes. To determine the molecular mechanisms involved and to explore whether such epigenetic regulations could play a role in early development, we used a cell culture system as close as possible to the embryonic state. We report efficient cardiac differentiation of embryonic stem (ES) cells induced by small non-coding RNAs with sequences of Cdk9, a key player in cardiomyocyte differentiation. Transfer of oligoribonucleotides representing parts of the Cdk9 mRNA into ES and mouse embryo fibroblast cultures resulted in upregulation of transcription. Dependency on Argonaute proteins and endogenous antisense transcripts indicated that the inducer oligoribonucleotides were processed by the RNAi machinery. Upregulation of Cdk9 expression resulted in increased efficiency of cardiac differentiation suggesting a potential tool for stem cell-based regenerative medicine. |
format | Online Article Text |
id | pubmed-5292948 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-52929482017-02-10 Small RNA-directed epigenetic programming of embryonic stem cell cardiac differentiation Ghanbarian, Hossein Wagner, Nicole Michiels, Jean-François Cuzin, François Wagner, Kay-Dietrich Rassoulzadegan, Minoo Sci Rep Article Microinjection of small noncoding RNAs in one-cell embryos was reported in several instances to result in transcriptional activation of target genes. To determine the molecular mechanisms involved and to explore whether such epigenetic regulations could play a role in early development, we used a cell culture system as close as possible to the embryonic state. We report efficient cardiac differentiation of embryonic stem (ES) cells induced by small non-coding RNAs with sequences of Cdk9, a key player in cardiomyocyte differentiation. Transfer of oligoribonucleotides representing parts of the Cdk9 mRNA into ES and mouse embryo fibroblast cultures resulted in upregulation of transcription. Dependency on Argonaute proteins and endogenous antisense transcripts indicated that the inducer oligoribonucleotides were processed by the RNAi machinery. Upregulation of Cdk9 expression resulted in increased efficiency of cardiac differentiation suggesting a potential tool for stem cell-based regenerative medicine. Nature Publishing Group 2017-02-06 /pmc/articles/PMC5292948/ /pubmed/28165496 http://dx.doi.org/10.1038/srep41799 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Ghanbarian, Hossein Wagner, Nicole Michiels, Jean-François Cuzin, François Wagner, Kay-Dietrich Rassoulzadegan, Minoo Small RNA-directed epigenetic programming of embryonic stem cell cardiac differentiation |
title | Small RNA-directed epigenetic programming of embryonic stem cell cardiac differentiation |
title_full | Small RNA-directed epigenetic programming of embryonic stem cell cardiac differentiation |
title_fullStr | Small RNA-directed epigenetic programming of embryonic stem cell cardiac differentiation |
title_full_unstemmed | Small RNA-directed epigenetic programming of embryonic stem cell cardiac differentiation |
title_short | Small RNA-directed epigenetic programming of embryonic stem cell cardiac differentiation |
title_sort | small rna-directed epigenetic programming of embryonic stem cell cardiac differentiation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5292948/ https://www.ncbi.nlm.nih.gov/pubmed/28165496 http://dx.doi.org/10.1038/srep41799 |
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