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L1 retrotransposon expression in circulating tumor cells

Long interspersed nuclear element 1 (LINE-1 or L1) belongs to the non-long terminal repeat (non-LTR) retrotransposon family, which has been implicated in carcinogenesis and disease progression. Circulating tumor cells (CTCs) are also known to be involved in cancer progression. The present study aime...

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Autores principales: Papasotiriou, Ioannis, Pantopikou, Katerina, Apostolou, Panagiotis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5293242/
https://www.ncbi.nlm.nih.gov/pubmed/28166262
http://dx.doi.org/10.1371/journal.pone.0171466
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author Papasotiriou, Ioannis
Pantopikou, Katerina
Apostolou, Panagiotis
author_facet Papasotiriou, Ioannis
Pantopikou, Katerina
Apostolou, Panagiotis
author_sort Papasotiriou, Ioannis
collection PubMed
description Long interspersed nuclear element 1 (LINE-1 or L1) belongs to the non-long terminal repeat (non-LTR) retrotransposon family, which has been implicated in carcinogenesis and disease progression. Circulating tumor cells (CTCs) are also known to be involved in cancer progression. The present study aimed to compare the L1 expression between circulating tumor cells and non-cancerous samples. Blood samples were collected from 10 healthy individuals and 22 patients with different types of cancer. The whole blood cells were isolated using enrichment protocols and the DNA and RNA were extracted. RT-qPCR was performed for L1-ORF1 (open reading frame 1) and L1-ORF2, using 18S rRNA as the reference gene. The data were analyzed with the Livak method and statistical analyses were carried out with the Mann-Whitney and Kruskal-Wallis tests. In parallel with the above molecular biology experiments, FISH experiments were performed on the interphase nuclei of the cells for the detection of ORF2 RNA. DNA analysis revealed the presence of both ORF1 and ORF2 in all samples. RNA expression experiments demonstrated that ORF1 was not expressed in all samples, while ORF2 was expressed at varying levels in the non-cancer samples and the samples representing the different cancer types. A significant difference in ORF2 expression was observed between the CTCs and non-cancer samples (p = 0,00043), and significant differences were also observed between normal and lung (p = 0,034), pancreatic (p = 0,022), prostate (p = 0,014), and unknown primary of origin (p = 0,0039) cancer samples. Cytogenetic analysis revealed higher levels of ORF2 in the nuclei of CTCs than in normal samples. This study highlights the significant difference in L1-ORF2 expression between CTCs and normal samples. The increased expression levels observed for CTCs may be correlated with the characteristic features of these cells.
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spelling pubmed-52932422017-02-17 L1 retrotransposon expression in circulating tumor cells Papasotiriou, Ioannis Pantopikou, Katerina Apostolou, Panagiotis PLoS One Research Article Long interspersed nuclear element 1 (LINE-1 or L1) belongs to the non-long terminal repeat (non-LTR) retrotransposon family, which has been implicated in carcinogenesis and disease progression. Circulating tumor cells (CTCs) are also known to be involved in cancer progression. The present study aimed to compare the L1 expression between circulating tumor cells and non-cancerous samples. Blood samples were collected from 10 healthy individuals and 22 patients with different types of cancer. The whole blood cells were isolated using enrichment protocols and the DNA and RNA were extracted. RT-qPCR was performed for L1-ORF1 (open reading frame 1) and L1-ORF2, using 18S rRNA as the reference gene. The data were analyzed with the Livak method and statistical analyses were carried out with the Mann-Whitney and Kruskal-Wallis tests. In parallel with the above molecular biology experiments, FISH experiments were performed on the interphase nuclei of the cells for the detection of ORF2 RNA. DNA analysis revealed the presence of both ORF1 and ORF2 in all samples. RNA expression experiments demonstrated that ORF1 was not expressed in all samples, while ORF2 was expressed at varying levels in the non-cancer samples and the samples representing the different cancer types. A significant difference in ORF2 expression was observed between the CTCs and non-cancer samples (p = 0,00043), and significant differences were also observed between normal and lung (p = 0,034), pancreatic (p = 0,022), prostate (p = 0,014), and unknown primary of origin (p = 0,0039) cancer samples. Cytogenetic analysis revealed higher levels of ORF2 in the nuclei of CTCs than in normal samples. This study highlights the significant difference in L1-ORF2 expression between CTCs and normal samples. The increased expression levels observed for CTCs may be correlated with the characteristic features of these cells. Public Library of Science 2017-02-06 /pmc/articles/PMC5293242/ /pubmed/28166262 http://dx.doi.org/10.1371/journal.pone.0171466 Text en © 2017 Papasotiriou et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Papasotiriou, Ioannis
Pantopikou, Katerina
Apostolou, Panagiotis
L1 retrotransposon expression in circulating tumor cells
title L1 retrotransposon expression in circulating tumor cells
title_full L1 retrotransposon expression in circulating tumor cells
title_fullStr L1 retrotransposon expression in circulating tumor cells
title_full_unstemmed L1 retrotransposon expression in circulating tumor cells
title_short L1 retrotransposon expression in circulating tumor cells
title_sort l1 retrotransposon expression in circulating tumor cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5293242/
https://www.ncbi.nlm.nih.gov/pubmed/28166262
http://dx.doi.org/10.1371/journal.pone.0171466
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