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Approach to determine the diversity of Legionella species by nested PCR-DGGE in aquatic environments

In this study, we describe a nested PCR-DGGE strategy to detect Legionella communities from river water samples. The nearly full-length 16S rRNA gene was amplified using bacterial primer in the first step. After, the amplicons were employed as DNA templates in the second PCR using Legionella specifi...

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Detalles Bibliográficos
Autores principales:	Huang, Wen-Chien, Tsai, Hsin-Chi, Tao, Chi-Wei, Chen, Jung-Sheng, Shih, Yi-Jia, Kao, Po-Min, Huang, Tung-Yi, Hsu, Bing-Mu
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2017
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5293244/ https://www.ncbi.nlm.nih.gov/pubmed/28166249 http://dx.doi.org/10.1371/journal.pone.0170992

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