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Standardization of Light Scattering Measurement in Conjunction With Immunochemical Analysis
Light scattering methods for the physical analysis of synthetic and biological polymers necessitates the use of scattering standards and absolute light scattering measurements. Standardization has not been employed when light scattering has been used to monitor immunochemical reactions using a kinet...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
[Gaithersburg, MD] : U.S. Dept. of Commerce, National Institute of Standards and Technology
1976
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5293526/ https://www.ncbi.nlm.nih.gov/pubmed/32196281 http://dx.doi.org/10.6028/jres.080A.059 |
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author | Buffone, Gregory J. |
author_facet | Buffone, Gregory J. |
author_sort | Buffone, Gregory J. |
collection | PubMed |
description | Light scattering methods for the physical analysis of synthetic and biological polymers necessitates the use of scattering standards and absolute light scattering measurements. Standardization has not been employed when light scattering has been used to monitor immunochemical reactions using a kinetic or thermodynamic mode. The concentration of a specific protein present in a complex matrix such as urine, serum or cerebrospinal fluid, is measured by reacting the protein of interest with its specific antibody and then measuring the excess light scattering of the solution produced by the formation of antigen antibody complexes. The lack of established light scattering standards in the area of immunochemical measurements makes instrumental quality control difficult and has hindered direct comparison of data among investigators. Both solid and liquid light scattering standards would be necessary to encompass the wide range of instrumentation currently in use. Several solid standards which have been used in the past include reflecting diffusers such as vitrolite, magnesium carbonate crystals with a ground surface, magnesium oxide coatings on magnesium carbonate crystal, casein paint on vitrolite, and solid opal glass transmitting diffusers such as flashed opal glass and solid opal glass. These standards, while applicable to manual light scattering photometers, are not suitable for recently developed automated instrumentation. Liquid standards in the form of Ludox(®), solutions of polystyrene, suspensions of small diameter latex spheres and even pure organic solvents could be used more easily with the continuous flow and discrete automated analyzers. The introduction of instrumental standards at this level of analysis would result in improved overall quality control and facilitate data and method comparison between laboratories. |
format | Online Article Text |
id | pubmed-5293526 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1976 |
publisher | [Gaithersburg, MD] : U.S. Dept. of Commerce, National Institute of Standards and Technology |
record_format | MEDLINE/PubMed |
spelling | pubmed-52935262020-03-18 Standardization of Light Scattering Measurement in Conjunction With Immunochemical Analysis Buffone, Gregory J. J Res Natl Bur Stand A Phys Chem Article Light scattering methods for the physical analysis of synthetic and biological polymers necessitates the use of scattering standards and absolute light scattering measurements. Standardization has not been employed when light scattering has been used to monitor immunochemical reactions using a kinetic or thermodynamic mode. The concentration of a specific protein present in a complex matrix such as urine, serum or cerebrospinal fluid, is measured by reacting the protein of interest with its specific antibody and then measuring the excess light scattering of the solution produced by the formation of antigen antibody complexes. The lack of established light scattering standards in the area of immunochemical measurements makes instrumental quality control difficult and has hindered direct comparison of data among investigators. Both solid and liquid light scattering standards would be necessary to encompass the wide range of instrumentation currently in use. Several solid standards which have been used in the past include reflecting diffusers such as vitrolite, magnesium carbonate crystals with a ground surface, magnesium oxide coatings on magnesium carbonate crystal, casein paint on vitrolite, and solid opal glass transmitting diffusers such as flashed opal glass and solid opal glass. These standards, while applicable to manual light scattering photometers, are not suitable for recently developed automated instrumentation. Liquid standards in the form of Ludox(®), solutions of polystyrene, suspensions of small diameter latex spheres and even pure organic solvents could be used more easily with the continuous flow and discrete automated analyzers. The introduction of instrumental standards at this level of analysis would result in improved overall quality control and facilitate data and method comparison between laboratories. [Gaithersburg, MD] : U.S. Dept. of Commerce, National Institute of Standards and Technology 1976 1976-08-01 /pmc/articles/PMC5293526/ /pubmed/32196281 http://dx.doi.org/10.6028/jres.080A.059 Text en https://creativecommons.org/publicdomain/zero/1.0/ The Journal of Research of the National Bureau of Standards Section A is a publication of the U.S. Government. The papers are in the public domain and are not subject to copyright in the United States. Articles from J Res may contain photographs or illustrations copyrighted by other commercial organizations or individuals that may not be used without obtaining prior approval from the holder of the copyright. |
spellingShingle | Article Buffone, Gregory J. Standardization of Light Scattering Measurement in Conjunction With Immunochemical Analysis |
title | Standardization of Light Scattering Measurement in Conjunction With Immunochemical Analysis |
title_full | Standardization of Light Scattering Measurement in Conjunction With Immunochemical Analysis |
title_fullStr | Standardization of Light Scattering Measurement in Conjunction With Immunochemical Analysis |
title_full_unstemmed | Standardization of Light Scattering Measurement in Conjunction With Immunochemical Analysis |
title_short | Standardization of Light Scattering Measurement in Conjunction With Immunochemical Analysis |
title_sort | standardization of light scattering measurement in conjunction with immunochemical analysis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5293526/ https://www.ncbi.nlm.nih.gov/pubmed/32196281 http://dx.doi.org/10.6028/jres.080A.059 |
work_keys_str_mv | AT buffonegregoryj standardizationoflightscatteringmeasurementinconjunctionwithimmunochemicalanalysis |