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Expression signature of lncRNAs and their potential roles in cardiac fibrosis of post-infarct mice
The present study aimed to investigate whether long non-coding RNAs (lncRNAs) are involved in cardiac fibrogenesis induced by myocardial infarction (MI). The differentially expressed lncRNAs and mRNAs in peri-infarct region of mice 4 weeks after MI were selected for bioinformatic analysis including...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Portland Press Ltd.
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5293569/ https://www.ncbi.nlm.nih.gov/pubmed/27129287 http://dx.doi.org/10.1042/BSR20150278 |
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author | Qu, Xuefeng Song, Xiaotong Yuan, Wei Shu, You Wang, Yuying Zhao, Xuyun Gao, Ming Lu, Renzhong Luo, Shenjian Zhao, Wei Zhang, Yue Sun, Lihua Lu, Yanjie |
author_facet | Qu, Xuefeng Song, Xiaotong Yuan, Wei Shu, You Wang, Yuying Zhao, Xuyun Gao, Ming Lu, Renzhong Luo, Shenjian Zhao, Wei Zhang, Yue Sun, Lihua Lu, Yanjie |
author_sort | Qu, Xuefeng |
collection | PubMed |
description | The present study aimed to investigate whether long non-coding RNAs (lncRNAs) are involved in cardiac fibrogenesis induced by myocardial infarction (MI). The differentially expressed lncRNAs and mRNAs in peri-infarct region of mice 4 weeks after MI were selected for bioinformatic analysis including gene ontology (GO) enrichment, pathway and network analysis. Left ventricular tissue levels of lncRNAs and mRNAs were compared between MI and sham control mice, using a false discovery rate (FDR) of <5%. Out of 55000 lncRNAs detected, 263 were significantly up-regulated and 282 down-regulated. Out of 23000 mRNAs detected, 142 were significantly up-regulated and 67 down-regulated. Among the differentially expressed lncRNAs, 53 were up-regulated by ≥2.0-fold change and 37 down-regulated by ≤0.5-fold change. Nine up-regulated and five down-regulated lncRNAs were randomly selected for quantitative real-time PCR (qRT-PCR) verification. GO and pathway analyses revealed 173 correlated lncRNA–mRNA pairs for 57 differentially expressed lncRNAs and 20 differentially expressed genes which are related to the development of cardiac fibrosis. We identified TGF-β(3) as the top-ranked gene, a critical component of the transforming growth factor-β (TGF-β) and mitogen activated protein kinase (MAPK) signalling pathways in cardiac fibrosis. NONMMUT022554 was identified as the top-ranked lncRNA, positively correlated with six up-regulated genes, which are involved in the extracellular matrix (ECM)–receptor interactions and the phosphoinositid-3 kinase/protein kinase B (PI3K-Akt) signalling pathway. Our study has identified the expression signature of lncRNAs in cardiac fibrosis induced by MI and unravelled the possible involvement of the deregulated lncRNAs in cardiac fibrosis and the associated pathological processes. |
format | Online Article Text |
id | pubmed-5293569 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Portland Press Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-52935692017-02-14 Expression signature of lncRNAs and their potential roles in cardiac fibrosis of post-infarct mice Qu, Xuefeng Song, Xiaotong Yuan, Wei Shu, You Wang, Yuying Zhao, Xuyun Gao, Ming Lu, Renzhong Luo, Shenjian Zhao, Wei Zhang, Yue Sun, Lihua Lu, Yanjie Biosci Rep Original Papers The present study aimed to investigate whether long non-coding RNAs (lncRNAs) are involved in cardiac fibrogenesis induced by myocardial infarction (MI). The differentially expressed lncRNAs and mRNAs in peri-infarct region of mice 4 weeks after MI were selected for bioinformatic analysis including gene ontology (GO) enrichment, pathway and network analysis. Left ventricular tissue levels of lncRNAs and mRNAs were compared between MI and sham control mice, using a false discovery rate (FDR) of <5%. Out of 55000 lncRNAs detected, 263 were significantly up-regulated and 282 down-regulated. Out of 23000 mRNAs detected, 142 were significantly up-regulated and 67 down-regulated. Among the differentially expressed lncRNAs, 53 were up-regulated by ≥2.0-fold change and 37 down-regulated by ≤0.5-fold change. Nine up-regulated and five down-regulated lncRNAs were randomly selected for quantitative real-time PCR (qRT-PCR) verification. GO and pathway analyses revealed 173 correlated lncRNA–mRNA pairs for 57 differentially expressed lncRNAs and 20 differentially expressed genes which are related to the development of cardiac fibrosis. We identified TGF-β(3) as the top-ranked gene, a critical component of the transforming growth factor-β (TGF-β) and mitogen activated protein kinase (MAPK) signalling pathways in cardiac fibrosis. NONMMUT022554 was identified as the top-ranked lncRNA, positively correlated with six up-regulated genes, which are involved in the extracellular matrix (ECM)–receptor interactions and the phosphoinositid-3 kinase/protein kinase B (PI3K-Akt) signalling pathway. Our study has identified the expression signature of lncRNAs in cardiac fibrosis induced by MI and unravelled the possible involvement of the deregulated lncRNAs in cardiac fibrosis and the associated pathological processes. Portland Press Ltd. 2016-06-03 /pmc/articles/PMC5293569/ /pubmed/27129287 http://dx.doi.org/10.1042/BSR20150278 Text en © 2016 The Author(s) http://creativecommons.org/licenses/by/4.0/ This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution Licence 4.0 (CC BY) (http://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Original Papers Qu, Xuefeng Song, Xiaotong Yuan, Wei Shu, You Wang, Yuying Zhao, Xuyun Gao, Ming Lu, Renzhong Luo, Shenjian Zhao, Wei Zhang, Yue Sun, Lihua Lu, Yanjie Expression signature of lncRNAs and their potential roles in cardiac fibrosis of post-infarct mice |
title | Expression signature of lncRNAs and their potential roles in cardiac fibrosis of post-infarct mice |
title_full | Expression signature of lncRNAs and their potential roles in cardiac fibrosis of post-infarct mice |
title_fullStr | Expression signature of lncRNAs and their potential roles in cardiac fibrosis of post-infarct mice |
title_full_unstemmed | Expression signature of lncRNAs and their potential roles in cardiac fibrosis of post-infarct mice |
title_short | Expression signature of lncRNAs and their potential roles in cardiac fibrosis of post-infarct mice |
title_sort | expression signature of lncrnas and their potential roles in cardiac fibrosis of post-infarct mice |
topic | Original Papers |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5293569/ https://www.ncbi.nlm.nih.gov/pubmed/27129287 http://dx.doi.org/10.1042/BSR20150278 |
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