Increased expression of SET domain-containing proteins and decreased expression of Rad51 in different classes of renal cell carcinoma

In the present study, we aimed to examine whether SET domain-containing methyltransferases are up-regulated in different classes of renal cell carcinoma. We immunoblotted against SET domain and quantified the expression of these modular domains. Furthermore, we examined the expression of Rad51, the...

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Autores principales: Liu, Si, Li, Yiyang, Xu, Hongmei, Wang, Kaichen, Li, Nan, Li, Jia, Sun, Tao, Xu, Ying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2016
Materias:
Original Papers
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5293581/
https://www.ncbi.nlm.nih.gov/pubmed/27170370
http://dx.doi.org/10.1042/BSR20160122
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author Liu, Si
Li, Yiyang
Xu, Hongmei
Wang, Kaichen
Li, Nan
Li, Jia
Sun, Tao
Xu, Ying
author_facet Liu, Si
Li, Yiyang
Xu, Hongmei
Wang, Kaichen
Li, Nan
Li, Jia
Sun, Tao
Xu, Ying
author_sort Liu, Si
collection PubMed
description In the present study, we aimed to examine whether SET domain-containing methyltransferases are up-regulated in different classes of renal cell carcinoma. We immunoblotted against SET domain and quantified the expression of these modular domains. Furthermore, we examined the expression of Rad51, the key protein that confers genomic stability. There was enhanced expression of SET domain-containing histone methyltransferases in whole lysates of all classes of renal carcinoma. In metastatic high grade clear cell carcinoma, this expression was more pronounced. Though we could not demonstrate direct correlation, we showed that epigenetic modification by methylation is associated with decreased genomic translation of Rad51.
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spelling pubmed-52935812017-02-14 Increased expression of SET domain-containing proteins and decreased expression of Rad51 in different classes of renal cell carcinoma Liu, Si Li, Yiyang Xu, Hongmei Wang, Kaichen Li, Nan Li, Jia Sun, Tao Xu, Ying Biosci Rep Original Papers In the present study, we aimed to examine whether SET domain-containing methyltransferases are up-regulated in different classes of renal cell carcinoma. We immunoblotted against SET domain and quantified the expression of these modular domains. Furthermore, we examined the expression of Rad51, the key protein that confers genomic stability. There was enhanced expression of SET domain-containing histone methyltransferases in whole lysates of all classes of renal carcinoma. In metastatic high grade clear cell carcinoma, this expression was more pronounced. Though we could not demonstrate direct correlation, we showed that epigenetic modification by methylation is associated with decreased genomic translation of Rad51. Portland Press Ltd. 2016-06-30 /pmc/articles/PMC5293581/ /pubmed/27170370 http://dx.doi.org/10.1042/BSR20160122 Text en © 2016 The Author(s) http://creativecommons.org/licenses/by/4.0/ This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution Licence 4.0 (CC BY) (http://creativecommons.org/licenses/by/4.0/) .
spellingShingle Original Papers
Liu, Si
Li, Yiyang
Xu, Hongmei
Wang, Kaichen
Li, Nan
Li, Jia
Sun, Tao
Xu, Ying
Increased expression of SET domain-containing proteins and decreased expression of Rad51 in different classes of renal cell carcinoma
title Increased expression of SET domain-containing proteins and decreased expression of Rad51 in different classes of renal cell carcinoma
title_full Increased expression of SET domain-containing proteins and decreased expression of Rad51 in different classes of renal cell carcinoma
title_fullStr Increased expression of SET domain-containing proteins and decreased expression of Rad51 in different classes of renal cell carcinoma
title_full_unstemmed Increased expression of SET domain-containing proteins and decreased expression of Rad51 in different classes of renal cell carcinoma
title_short Increased expression of SET domain-containing proteins and decreased expression of Rad51 in different classes of renal cell carcinoma
title_sort increased expression of set domain-containing proteins and decreased expression of rad51 in different classes of renal cell carcinoma
topic Original Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5293581/
https://www.ncbi.nlm.nih.gov/pubmed/27170370
http://dx.doi.org/10.1042/BSR20160122
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