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Resveratrol ameliorates renal injury in spontaneously hypertensive rats by inhibiting renal micro-inflammation

Micro-inflammation plays an important role in the pathogenesis of spontaneously hypertensive rat (SHR). In the present study, we investigated the therapeutic potential of resveratrol (RSV), a polyphenol with anti-fibrosis activity in hypertensive renal damage model. In SHR renal damage model, RSV tr...

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Autores principales: Xue, Hai-Yan, Yuan, Li, Cao, Ying-Jie, Fan, Ya-Ping, Chen, Xiao-Lan, Huang, Xin-Zhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5293588/
https://www.ncbi.nlm.nih.gov/pubmed/27129290
http://dx.doi.org/10.1042/BSR20160035
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author Xue, Hai-Yan
Yuan, Li
Cao, Ying-Jie
Fan, Ya-Ping
Chen, Xiao-Lan
Huang, Xin-Zhong
author_facet Xue, Hai-Yan
Yuan, Li
Cao, Ying-Jie
Fan, Ya-Ping
Chen, Xiao-Lan
Huang, Xin-Zhong
author_sort Xue, Hai-Yan
collection PubMed
description Micro-inflammation plays an important role in the pathogenesis of spontaneously hypertensive rat (SHR). In the present study, we investigated the therapeutic potential of resveratrol (RSV), a polyphenol with anti-fibrosis activity in hypertensive renal damage model. In SHR renal damage model, RSV treatment blunted the increase in urine albumin excretion, urinary β(2)-microglobulin (β(2)-MG), attenuated the decrease in creatinine clearance rate (CCR). The glomerular sclerosis index (1.54±0.33 compared with 0.36±0.07) and tubulointerstitial fibrosis (1.57±0.31 compared with 0.19±0.04) were significantly higher in SHRs compared with Wistar Kyoto rats (WKYs), which were significantly lower by RSV treatment. The increases in mesangium accumulation and the expression of renal collagen type I (Col I), fibronectin (Fn), plasminogen activator inhibitor-1 (PAI-1) and transforming growth factor-β1 (TGF-β1) in SHR were also reduced by RSV treatment. Nuclear factor κB (NF-κB) expression was increased in the cytoplasm and nuclei of the SHR kidneys, which was significantly decreased by RSV treatment. Furthermore, the protein level of IκB-α significantly decreased in the kidneys of the SHR when compared with the WKYs. RSV treatment partially restored the decreased IκB-α level. In SHR kidney, increased expression of interleukin-6 (IL-6), intercellular adhesion molecule-1 (ICAM-1) and monocyte chemoattractant protein 1 (MCP-1) were observed. These changes were attenuated by RSV treatment. No changes in blood pressure were detected between SHR group and SHR + RSV group. Taken together, the present study demonstrated that RSV treatment may significantly attenuate renal damage in the SHR model of chronic kidney disease (CKD). The renal protective effect is associated with inhibition of IL-6, ICAM-1 and MCP-1 expression via the regulation of the nuclear translocation of NF-κB, which suggesting that micro-inflammation may be a potential therapeutic target of hypertensive renal damage.
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spelling pubmed-52935882017-02-14 Resveratrol ameliorates renal injury in spontaneously hypertensive rats by inhibiting renal micro-inflammation Xue, Hai-Yan Yuan, Li Cao, Ying-Jie Fan, Ya-Ping Chen, Xiao-Lan Huang, Xin-Zhong Biosci Rep Original Papers Micro-inflammation plays an important role in the pathogenesis of spontaneously hypertensive rat (SHR). In the present study, we investigated the therapeutic potential of resveratrol (RSV), a polyphenol with anti-fibrosis activity in hypertensive renal damage model. In SHR renal damage model, RSV treatment blunted the increase in urine albumin excretion, urinary β(2)-microglobulin (β(2)-MG), attenuated the decrease in creatinine clearance rate (CCR). The glomerular sclerosis index (1.54±0.33 compared with 0.36±0.07) and tubulointerstitial fibrosis (1.57±0.31 compared with 0.19±0.04) were significantly higher in SHRs compared with Wistar Kyoto rats (WKYs), which were significantly lower by RSV treatment. The increases in mesangium accumulation and the expression of renal collagen type I (Col I), fibronectin (Fn), plasminogen activator inhibitor-1 (PAI-1) and transforming growth factor-β1 (TGF-β1) in SHR were also reduced by RSV treatment. Nuclear factor κB (NF-κB) expression was increased in the cytoplasm and nuclei of the SHR kidneys, which was significantly decreased by RSV treatment. Furthermore, the protein level of IκB-α significantly decreased in the kidneys of the SHR when compared with the WKYs. RSV treatment partially restored the decreased IκB-α level. In SHR kidney, increased expression of interleukin-6 (IL-6), intercellular adhesion molecule-1 (ICAM-1) and monocyte chemoattractant protein 1 (MCP-1) were observed. These changes were attenuated by RSV treatment. No changes in blood pressure were detected between SHR group and SHR + RSV group. Taken together, the present study demonstrated that RSV treatment may significantly attenuate renal damage in the SHR model of chronic kidney disease (CKD). The renal protective effect is associated with inhibition of IL-6, ICAM-1 and MCP-1 expression via the regulation of the nuclear translocation of NF-κB, which suggesting that micro-inflammation may be a potential therapeutic target of hypertensive renal damage. Portland Press Ltd. 2016-06-03 /pmc/articles/PMC5293588/ /pubmed/27129290 http://dx.doi.org/10.1042/BSR20160035 Text en © 2016 The Author(s) http://creativecommons.org/licenses/by/4.0/ This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution Licence 4.0 (CC BY) (http://creativecommons.org/licenses/by/4.0/) .
spellingShingle Original Papers
Xue, Hai-Yan
Yuan, Li
Cao, Ying-Jie
Fan, Ya-Ping
Chen, Xiao-Lan
Huang, Xin-Zhong
Resveratrol ameliorates renal injury in spontaneously hypertensive rats by inhibiting renal micro-inflammation
title Resveratrol ameliorates renal injury in spontaneously hypertensive rats by inhibiting renal micro-inflammation
title_full Resveratrol ameliorates renal injury in spontaneously hypertensive rats by inhibiting renal micro-inflammation
title_fullStr Resveratrol ameliorates renal injury in spontaneously hypertensive rats by inhibiting renal micro-inflammation
title_full_unstemmed Resveratrol ameliorates renal injury in spontaneously hypertensive rats by inhibiting renal micro-inflammation
title_short Resveratrol ameliorates renal injury in spontaneously hypertensive rats by inhibiting renal micro-inflammation
title_sort resveratrol ameliorates renal injury in spontaneously hypertensive rats by inhibiting renal micro-inflammation
topic Original Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5293588/
https://www.ncbi.nlm.nih.gov/pubmed/27129290
http://dx.doi.org/10.1042/BSR20160035
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