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Cocktail supplement with rosiglitazone: a novel inducer for chicken preadipocyte differentiation in vitro

The preadipocyte differentiation biological process involves a cascade of transcriptional events that culminates in the expression of peroxisome proliferator-activated receptor (PPAR) γ. The differentiation cocktail [insulin (INS), dexamethasone (DEX) and isobutylmethylxanthine (IBMX)] can induce pr...

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Autores principales: Cheng, Bohan, Wu, Mengqi, Xu, Songsong, Zhang, Xinyang, Wang, Yuxiang, Wang, Ning, Leng, Li, Li, Hui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5293590/
https://www.ncbi.nlm.nih.gov/pubmed/27638500
http://dx.doi.org/10.1042/BSR20160049
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author Cheng, Bohan
Wu, Mengqi
Xu, Songsong
Zhang, Xinyang
Wang, Yuxiang
Wang, Ning
Leng, Li
Li, Hui
author_facet Cheng, Bohan
Wu, Mengqi
Xu, Songsong
Zhang, Xinyang
Wang, Yuxiang
Wang, Ning
Leng, Li
Li, Hui
author_sort Cheng, Bohan
collection PubMed
description The preadipocyte differentiation biological process involves a cascade of transcriptional events that culminates in the expression of peroxisome proliferator-activated receptor (PPAR) γ. The differentiation cocktail [insulin (INS), dexamethasone (DEX) and isobutylmethylxanthine (IBMX)] can induce preadipocyte differentiation in mammals, but it is insufficient for chicken (Gallus gallus) adipogenesis. Oleate can induce chicken preadipocyte differentiation, but these differentiated preadipocytes may not be fully functional. The objective of the current study was to evaluate whether chicken preadipocytes can be induced to mature adipocytes by a novel induction method using differentiation cocktail supplemented with PPARγ agonist(s). Chicken preadipocytes cultured in cocktail supplemented with rosiglitazone or troglitazone resulted in a marked increase in lipid droplet accumulation (P<0.05), glycerol-3-phosphate dehydrogenase (GPDH) activity (P<0.05), mRNA expression level of adipocyte fatty acid-binding protein (aP2; P<0.05), G(0)/G(1) switch gene 2 (G0S2; P<0.05) and lipolysis (P<0.05). In addition, supplementation of the cocktail with rosiglitazone promoted PPARγ mRNA expression (P<0.05). In conclusion, our data indicated that chicken preadipocytes can be induced to mature adipocytes using differentiation cocktail supplemented with rosiglitazone. The results of the present study provide a novel induction method for in vitro chicken preadipocyte differentiation.
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spelling pubmed-52935902017-02-14 Cocktail supplement with rosiglitazone: a novel inducer for chicken preadipocyte differentiation in vitro Cheng, Bohan Wu, Mengqi Xu, Songsong Zhang, Xinyang Wang, Yuxiang Wang, Ning Leng, Li Li, Hui Biosci Rep Original Papers The preadipocyte differentiation biological process involves a cascade of transcriptional events that culminates in the expression of peroxisome proliferator-activated receptor (PPAR) γ. The differentiation cocktail [insulin (INS), dexamethasone (DEX) and isobutylmethylxanthine (IBMX)] can induce preadipocyte differentiation in mammals, but it is insufficient for chicken (Gallus gallus) adipogenesis. Oleate can induce chicken preadipocyte differentiation, but these differentiated preadipocytes may not be fully functional. The objective of the current study was to evaluate whether chicken preadipocytes can be induced to mature adipocytes by a novel induction method using differentiation cocktail supplemented with PPARγ agonist(s). Chicken preadipocytes cultured in cocktail supplemented with rosiglitazone or troglitazone resulted in a marked increase in lipid droplet accumulation (P<0.05), glycerol-3-phosphate dehydrogenase (GPDH) activity (P<0.05), mRNA expression level of adipocyte fatty acid-binding protein (aP2; P<0.05), G(0)/G(1) switch gene 2 (G0S2; P<0.05) and lipolysis (P<0.05). In addition, supplementation of the cocktail with rosiglitazone promoted PPARγ mRNA expression (P<0.05). In conclusion, our data indicated that chicken preadipocytes can be induced to mature adipocytes using differentiation cocktail supplemented with rosiglitazone. The results of the present study provide a novel induction method for in vitro chicken preadipocyte differentiation. Portland Press Ltd. 2016-11-03 /pmc/articles/PMC5293590/ /pubmed/27638500 http://dx.doi.org/10.1042/BSR20160049 Text en © 2016 The Author(s) http://creativecommons.org/licenses/by/4.0/ This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution Licence 4.0 (CC BY) (http://creativecommons.org/licenses/by/4.0/) .
spellingShingle Original Papers
Cheng, Bohan
Wu, Mengqi
Xu, Songsong
Zhang, Xinyang
Wang, Yuxiang
Wang, Ning
Leng, Li
Li, Hui
Cocktail supplement with rosiglitazone: a novel inducer for chicken preadipocyte differentiation in vitro
title Cocktail supplement with rosiglitazone: a novel inducer for chicken preadipocyte differentiation in vitro
title_full Cocktail supplement with rosiglitazone: a novel inducer for chicken preadipocyte differentiation in vitro
title_fullStr Cocktail supplement with rosiglitazone: a novel inducer for chicken preadipocyte differentiation in vitro
title_full_unstemmed Cocktail supplement with rosiglitazone: a novel inducer for chicken preadipocyte differentiation in vitro
title_short Cocktail supplement with rosiglitazone: a novel inducer for chicken preadipocyte differentiation in vitro
title_sort cocktail supplement with rosiglitazone: a novel inducer for chicken preadipocyte differentiation in vitro
topic Original Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5293590/
https://www.ncbi.nlm.nih.gov/pubmed/27638500
http://dx.doi.org/10.1042/BSR20160049
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