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Staphylococcal aureus Enterotoxin C and Enterotoxin-Like L Associated with Post-partum Mastitis

Denmark is a low prevalence country with regard to methicillin resistant Staphylococcus aureus (MRSA). In 2008 and 2014, two neonatal wards in the Copenhagen area experienced outbreaks with a typical community acquired MRSA belonging to the same spa type and sequence type (t015:ST45) and both were P...

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Autores principales: Franck, Kristina T., Gumpert, Heidi, Olesen, Bente, Larsen, Anders R., Petersen, Andreas, Bangsborg, Jette, Albertsen, Per, Westh, Henrik, Bartels, Mette D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5293744/
https://www.ncbi.nlm.nih.gov/pubmed/28223977
http://dx.doi.org/10.3389/fmicb.2017.00173
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author Franck, Kristina T.
Gumpert, Heidi
Olesen, Bente
Larsen, Anders R.
Petersen, Andreas
Bangsborg, Jette
Albertsen, Per
Westh, Henrik
Bartels, Mette D.
author_facet Franck, Kristina T.
Gumpert, Heidi
Olesen, Bente
Larsen, Anders R.
Petersen, Andreas
Bangsborg, Jette
Albertsen, Per
Westh, Henrik
Bartels, Mette D.
author_sort Franck, Kristina T.
collection PubMed
description Denmark is a low prevalence country with regard to methicillin resistant Staphylococcus aureus (MRSA). In 2008 and 2014, two neonatal wards in the Copenhagen area experienced outbreaks with a typical community acquired MRSA belonging to the same spa type and sequence type (t015:ST45) and both were PVL and ACME negative. In outbreak 1, the isolates harbored SCCmec IVa and in outbreak 2 SCCmec V. The clinical presentation differed between the two outbreaks, as none of five MRSA positive mothers in outbreak 1 had mastitis vs. five of six MRSA positive mothers in outbreak 2 (p < 0.02). To investigate if whole-genome sequencing could identify virulence genes associated with mastitis, t015:ST45 isolates from Denmark (N = 101) were whole-genome sequenced. Sequence analysis confirmed two separate outbreaks with no sign of sustained spread into the community. Analysis of the accessory genome between isolates from the two outbreaks revealed a S. aureus pathogenicity island containing enterotoxin C and enterotoxin-like L only in isolates from outbreak 2. Enterotoxin C and enterotoxin-like L carrying S. aureus are associated with bovine mastitis and our findings indicate that these may also be important virulence factors for human mastitis.
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spelling pubmed-52937442017-02-21 Staphylococcal aureus Enterotoxin C and Enterotoxin-Like L Associated with Post-partum Mastitis Franck, Kristina T. Gumpert, Heidi Olesen, Bente Larsen, Anders R. Petersen, Andreas Bangsborg, Jette Albertsen, Per Westh, Henrik Bartels, Mette D. Front Microbiol Microbiology Denmark is a low prevalence country with regard to methicillin resistant Staphylococcus aureus (MRSA). In 2008 and 2014, two neonatal wards in the Copenhagen area experienced outbreaks with a typical community acquired MRSA belonging to the same spa type and sequence type (t015:ST45) and both were PVL and ACME negative. In outbreak 1, the isolates harbored SCCmec IVa and in outbreak 2 SCCmec V. The clinical presentation differed between the two outbreaks, as none of five MRSA positive mothers in outbreak 1 had mastitis vs. five of six MRSA positive mothers in outbreak 2 (p < 0.02). To investigate if whole-genome sequencing could identify virulence genes associated with mastitis, t015:ST45 isolates from Denmark (N = 101) were whole-genome sequenced. Sequence analysis confirmed two separate outbreaks with no sign of sustained spread into the community. Analysis of the accessory genome between isolates from the two outbreaks revealed a S. aureus pathogenicity island containing enterotoxin C and enterotoxin-like L only in isolates from outbreak 2. Enterotoxin C and enterotoxin-like L carrying S. aureus are associated with bovine mastitis and our findings indicate that these may also be important virulence factors for human mastitis. Frontiers Media S.A. 2017-02-07 /pmc/articles/PMC5293744/ /pubmed/28223977 http://dx.doi.org/10.3389/fmicb.2017.00173 Text en Copyright © 2017 Franck, Gumpert, Olesen, Larsen, Petersen, Bangsborg, Albertsen, Westh and Bartels. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Franck, Kristina T.
Gumpert, Heidi
Olesen, Bente
Larsen, Anders R.
Petersen, Andreas
Bangsborg, Jette
Albertsen, Per
Westh, Henrik
Bartels, Mette D.
Staphylococcal aureus Enterotoxin C and Enterotoxin-Like L Associated with Post-partum Mastitis
title Staphylococcal aureus Enterotoxin C and Enterotoxin-Like L Associated with Post-partum Mastitis
title_full Staphylococcal aureus Enterotoxin C and Enterotoxin-Like L Associated with Post-partum Mastitis
title_fullStr Staphylococcal aureus Enterotoxin C and Enterotoxin-Like L Associated with Post-partum Mastitis
title_full_unstemmed Staphylococcal aureus Enterotoxin C and Enterotoxin-Like L Associated with Post-partum Mastitis
title_short Staphylococcal aureus Enterotoxin C and Enterotoxin-Like L Associated with Post-partum Mastitis
title_sort staphylococcal aureus enterotoxin c and enterotoxin-like l associated with post-partum mastitis
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5293744/
https://www.ncbi.nlm.nih.gov/pubmed/28223977
http://dx.doi.org/10.3389/fmicb.2017.00173
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