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UFGT: The Key Enzyme Associated with the Petals Variegation in Japanese Apricot

Japanese apricot (Prunus mume Sieb.et Zucc.) is an important ornamental plant in China. One of the traits of petals color variegation is attractive, but its formation mechanism is unclear. In our study, RNA-seq technology was employed to characterize the transcriptome response to the mutation of “Fu...

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Autores principales: Wu, Xinxin, Gong, Qinghua, Ni, Xiaopeng, Zhou, Yong, Gao, Zhihong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5293763/
https://www.ncbi.nlm.nih.gov/pubmed/28223989
http://dx.doi.org/10.3389/fpls.2017.00108
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author Wu, Xinxin
Gong, Qinghua
Ni, Xiaopeng
Zhou, Yong
Gao, Zhihong
author_facet Wu, Xinxin
Gong, Qinghua
Ni, Xiaopeng
Zhou, Yong
Gao, Zhihong
author_sort Wu, Xinxin
collection PubMed
description Japanese apricot (Prunus mume Sieb.et Zucc.) is an important ornamental plant in China. One of the traits of petals color variegation is attractive, but its formation mechanism is unclear. In our study, RNA-seq technology was employed to characterize the transcriptome response to the mutation of “Fuban Tiaozhi” associated with petals variegation in Japanese apricot. As a result, 4,579,040 (white-flowered, WF) and 7,269,883 (red-flowered, RF) reads were mapped to P. persica genes, while 5,006,676 (WF) and 7,907,436 (RF) were mapped to P. persica genomes. There were 960 differentially expressed genes (DEGs) identified. Gene ontology analysis showed that these genes involved in 37 functional groups including 19 biological processes, 10 cellular components and eight molecular functions. Pathway enrichment annotation demonstrated that highly ranked genes were associated with flavonoid biosynthesis, anthocyanin biosynthesis, anthocyanins transports, plant hormone signal transduction, and transcriptional factors. The expression patterns part of them were validated by qRT-PCR. We found that UDP-glucose: flavonoid 3-O-glucosyltransferase (UFGT) gene showed differential expression pattern. The UFGT enzyme activities in RF had a significantly higher than that of WF and lower in the initial stage and increased when the red appeared in the petals, which is identical to the accumulation of anthocyanins. And we also validated the SNPs, leading to the nonsynonymous mutations, in the UFGT by Sanger sequencing which may affect the enzyme activity. In summary, our results provide molecular candidates for better understanding the mechanisms of the variegation in Japanese Apricot.
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spelling pubmed-52937632017-02-21 UFGT: The Key Enzyme Associated with the Petals Variegation in Japanese Apricot Wu, Xinxin Gong, Qinghua Ni, Xiaopeng Zhou, Yong Gao, Zhihong Front Plant Sci Plant Science Japanese apricot (Prunus mume Sieb.et Zucc.) is an important ornamental plant in China. One of the traits of petals color variegation is attractive, but its formation mechanism is unclear. In our study, RNA-seq technology was employed to characterize the transcriptome response to the mutation of “Fuban Tiaozhi” associated with petals variegation in Japanese apricot. As a result, 4,579,040 (white-flowered, WF) and 7,269,883 (red-flowered, RF) reads were mapped to P. persica genes, while 5,006,676 (WF) and 7,907,436 (RF) were mapped to P. persica genomes. There were 960 differentially expressed genes (DEGs) identified. Gene ontology analysis showed that these genes involved in 37 functional groups including 19 biological processes, 10 cellular components and eight molecular functions. Pathway enrichment annotation demonstrated that highly ranked genes were associated with flavonoid biosynthesis, anthocyanin biosynthesis, anthocyanins transports, plant hormone signal transduction, and transcriptional factors. The expression patterns part of them were validated by qRT-PCR. We found that UDP-glucose: flavonoid 3-O-glucosyltransferase (UFGT) gene showed differential expression pattern. The UFGT enzyme activities in RF had a significantly higher than that of WF and lower in the initial stage and increased when the red appeared in the petals, which is identical to the accumulation of anthocyanins. And we also validated the SNPs, leading to the nonsynonymous mutations, in the UFGT by Sanger sequencing which may affect the enzyme activity. In summary, our results provide molecular candidates for better understanding the mechanisms of the variegation in Japanese Apricot. Frontiers Media S.A. 2017-02-07 /pmc/articles/PMC5293763/ /pubmed/28223989 http://dx.doi.org/10.3389/fpls.2017.00108 Text en Copyright © 2017 Wu, Gong, Ni, Zhou and Gao. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Wu, Xinxin
Gong, Qinghua
Ni, Xiaopeng
Zhou, Yong
Gao, Zhihong
UFGT: The Key Enzyme Associated with the Petals Variegation in Japanese Apricot
title UFGT: The Key Enzyme Associated with the Petals Variegation in Japanese Apricot
title_full UFGT: The Key Enzyme Associated with the Petals Variegation in Japanese Apricot
title_fullStr UFGT: The Key Enzyme Associated with the Petals Variegation in Japanese Apricot
title_full_unstemmed UFGT: The Key Enzyme Associated with the Petals Variegation in Japanese Apricot
title_short UFGT: The Key Enzyme Associated with the Petals Variegation in Japanese Apricot
title_sort ufgt: the key enzyme associated with the petals variegation in japanese apricot
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5293763/
https://www.ncbi.nlm.nih.gov/pubmed/28223989
http://dx.doi.org/10.3389/fpls.2017.00108
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