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Embryonic and Postnatal Expression of Aryl Hydrocarbon Receptor mRNA in Mouse Brain

Aryl hydrocarbon receptor (AhR), a member of the basic helix-loop-helix-Per-Arnt-Sim transcription factor family, plays a critical role in the developing nervous system of invertebrates and vertebrates. Dioxin, a ubiquitous environmental pollutant, avidly binds to this receptor, and maternal exposur...

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Detalles Bibliográficos
Autores principales: Kimura, Eiki, Tohyama, Chiharu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5293765/
https://www.ncbi.nlm.nih.gov/pubmed/28223923
http://dx.doi.org/10.3389/fnana.2017.00004
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author Kimura, Eiki
Tohyama, Chiharu
author_facet Kimura, Eiki
Tohyama, Chiharu
author_sort Kimura, Eiki
collection PubMed
description Aryl hydrocarbon receptor (AhR), a member of the basic helix-loop-helix-Per-Arnt-Sim transcription factor family, plays a critical role in the developing nervous system of invertebrates and vertebrates. Dioxin, a ubiquitous environmental pollutant, avidly binds to this receptor, and maternal exposure to dioxin has been shown to impair higher brain functions and dendritic morphogenesis, possibly via an AhR-dependent mechanism. However, there is little information on AhR expression in the developing mammalian brain. To address this issue, the present study analyzed AhR mRNA expression in the brains of embryonic, juvenile, and adult mice by reverse transcription (RT)-PCR and in situ hybridization. In early brain development (embryonic day 12.5), AhR transcript was detected in the innermost cortical layer. The mRNA was also expressed in the hippocampus, cerebral cortex, cerebellum, olfactory bulb, and rostral migratory stream on embryonic day 18.5, postnatal days 3, 7, and 14, and in 12-week-old (adult) mice. Hippocampal expression was abundant in the CA1 and CA3 pyramidal and dentate gyrus granule cell layers, where expression level of AhR mRNA in 12-week old is higher than that in 7-day old. These results reveal temporal and spatial patterns of AhR mRNA expression in the mouse brain, providing the information that may contribute to the elucidation of the physiologic and toxicologic significance of AhR in the developing brain.
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spelling pubmed-52937652017-02-21 Embryonic and Postnatal Expression of Aryl Hydrocarbon Receptor mRNA in Mouse Brain Kimura, Eiki Tohyama, Chiharu Front Neuroanat Neuroanatomy Aryl hydrocarbon receptor (AhR), a member of the basic helix-loop-helix-Per-Arnt-Sim transcription factor family, plays a critical role in the developing nervous system of invertebrates and vertebrates. Dioxin, a ubiquitous environmental pollutant, avidly binds to this receptor, and maternal exposure to dioxin has been shown to impair higher brain functions and dendritic morphogenesis, possibly via an AhR-dependent mechanism. However, there is little information on AhR expression in the developing mammalian brain. To address this issue, the present study analyzed AhR mRNA expression in the brains of embryonic, juvenile, and adult mice by reverse transcription (RT)-PCR and in situ hybridization. In early brain development (embryonic day 12.5), AhR transcript was detected in the innermost cortical layer. The mRNA was also expressed in the hippocampus, cerebral cortex, cerebellum, olfactory bulb, and rostral migratory stream on embryonic day 18.5, postnatal days 3, 7, and 14, and in 12-week-old (adult) mice. Hippocampal expression was abundant in the CA1 and CA3 pyramidal and dentate gyrus granule cell layers, where expression level of AhR mRNA in 12-week old is higher than that in 7-day old. These results reveal temporal and spatial patterns of AhR mRNA expression in the mouse brain, providing the information that may contribute to the elucidation of the physiologic and toxicologic significance of AhR in the developing brain. Frontiers Media S.A. 2017-02-07 /pmc/articles/PMC5293765/ /pubmed/28223923 http://dx.doi.org/10.3389/fnana.2017.00004 Text en Copyright © 2017 Kimura and Tohyama. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroanatomy
Kimura, Eiki
Tohyama, Chiharu
Embryonic and Postnatal Expression of Aryl Hydrocarbon Receptor mRNA in Mouse Brain
title Embryonic and Postnatal Expression of Aryl Hydrocarbon Receptor mRNA in Mouse Brain
title_full Embryonic and Postnatal Expression of Aryl Hydrocarbon Receptor mRNA in Mouse Brain
title_fullStr Embryonic and Postnatal Expression of Aryl Hydrocarbon Receptor mRNA in Mouse Brain
title_full_unstemmed Embryonic and Postnatal Expression of Aryl Hydrocarbon Receptor mRNA in Mouse Brain
title_short Embryonic and Postnatal Expression of Aryl Hydrocarbon Receptor mRNA in Mouse Brain
title_sort embryonic and postnatal expression of aryl hydrocarbon receptor mrna in mouse brain
topic Neuroanatomy
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5293765/
https://www.ncbi.nlm.nih.gov/pubmed/28223923
http://dx.doi.org/10.3389/fnana.2017.00004
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