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PerC Manipulates Metabolism and Surface Antigens in Enteropathogenic Escherichia coli

Enteropathogenic Escherichia coli is an important cause of profuse, watery diarrhea in infants living in developing regions of the world. Typical strains of EPEC (tEPEC) possess a virulence plasmid, while related clinical isolates that lack the pEAF plasmid are termed atypical EPEC (aEPEC). tEPEC an...

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Autores principales: Mellies, Jay L., Platenkamp, Amy, Osborn, Jossef, Ben-Avi, Lily
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5293775/
https://www.ncbi.nlm.nih.gov/pubmed/28224117
http://dx.doi.org/10.3389/fcimb.2017.00032
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author Mellies, Jay L.
Platenkamp, Amy
Osborn, Jossef
Ben-Avi, Lily
author_facet Mellies, Jay L.
Platenkamp, Amy
Osborn, Jossef
Ben-Avi, Lily
author_sort Mellies, Jay L.
collection PubMed
description Enteropathogenic Escherichia coli is an important cause of profuse, watery diarrhea in infants living in developing regions of the world. Typical strains of EPEC (tEPEC) possess a virulence plasmid, while related clinical isolates that lack the pEAF plasmid are termed atypical EPEC (aEPEC). tEPEC and aEPEC tend to cause acute vs. more chronic type infections, respectively. The pEAF plasmid encodes an attachment factor as well as a regulatory operon, perABC. PerC, a poorly understood regulator, was previously shown to regulate expression of the type III secretion system through Ler. Here we elucidate the regulon of PerC using RNA sequencing analysis to better our understanding of the role of the pEAF in tEPEC infection. We demonstrate that PerC controls anaerobic metabolism by increasing expression of genes necessary for nitrate reduction. A tEPEC strain overexpressing PerC exhibited a growth advantage compared to a strain lacking this regulator, when grown anaerobically in the presence of nitrate, conditions mimicking the human intestine. We show that PerC strongly down-regulates type I fimbriae expression by manipulating fim phase variation. The quantities of a number of non-coding RNA molecules were altered by PerC. In sum, this protein controls niche adaptation, and could help to explain the function of the PerC homologs (Pch), many of which are encoded within prophages in related, Gram-negative pathogens.
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spelling pubmed-52937752017-02-21 PerC Manipulates Metabolism and Surface Antigens in Enteropathogenic Escherichia coli Mellies, Jay L. Platenkamp, Amy Osborn, Jossef Ben-Avi, Lily Front Cell Infect Microbiol Microbiology Enteropathogenic Escherichia coli is an important cause of profuse, watery diarrhea in infants living in developing regions of the world. Typical strains of EPEC (tEPEC) possess a virulence plasmid, while related clinical isolates that lack the pEAF plasmid are termed atypical EPEC (aEPEC). tEPEC and aEPEC tend to cause acute vs. more chronic type infections, respectively. The pEAF plasmid encodes an attachment factor as well as a regulatory operon, perABC. PerC, a poorly understood regulator, was previously shown to regulate expression of the type III secretion system through Ler. Here we elucidate the regulon of PerC using RNA sequencing analysis to better our understanding of the role of the pEAF in tEPEC infection. We demonstrate that PerC controls anaerobic metabolism by increasing expression of genes necessary for nitrate reduction. A tEPEC strain overexpressing PerC exhibited a growth advantage compared to a strain lacking this regulator, when grown anaerobically in the presence of nitrate, conditions mimicking the human intestine. We show that PerC strongly down-regulates type I fimbriae expression by manipulating fim phase variation. The quantities of a number of non-coding RNA molecules were altered by PerC. In sum, this protein controls niche adaptation, and could help to explain the function of the PerC homologs (Pch), many of which are encoded within prophages in related, Gram-negative pathogens. Frontiers Media S.A. 2017-02-07 /pmc/articles/PMC5293775/ /pubmed/28224117 http://dx.doi.org/10.3389/fcimb.2017.00032 Text en Copyright © 2017 Mellies, Platenkamp, Osborn and Ben-Avi. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Mellies, Jay L.
Platenkamp, Amy
Osborn, Jossef
Ben-Avi, Lily
PerC Manipulates Metabolism and Surface Antigens in Enteropathogenic Escherichia coli
title PerC Manipulates Metabolism and Surface Antigens in Enteropathogenic Escherichia coli
title_full PerC Manipulates Metabolism and Surface Antigens in Enteropathogenic Escherichia coli
title_fullStr PerC Manipulates Metabolism and Surface Antigens in Enteropathogenic Escherichia coli
title_full_unstemmed PerC Manipulates Metabolism and Surface Antigens in Enteropathogenic Escherichia coli
title_short PerC Manipulates Metabolism and Surface Antigens in Enteropathogenic Escherichia coli
title_sort perc manipulates metabolism and surface antigens in enteropathogenic escherichia coli
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5293775/
https://www.ncbi.nlm.nih.gov/pubmed/28224117
http://dx.doi.org/10.3389/fcimb.2017.00032
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