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Overexpression of Prunus mume Dehydrin Genes in Tobacco Enhances Tolerance to Cold and Drought

Dehydrins, known as group 2 or D-11 family late-embryogenesis-abundant (LEA) proteins, play important roles in plant growth and stress tolerance. Six dehydrin genes were previously identified from the genome of Prunus mume. In this study, five of them (PmLEA8, PmLEA10, PmLEA19, PmLEA20, and PmLEA29)...

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Autores principales: Bao, Fei, Du, Dongliang, An, Yang, Yang, Weiru, Wang, Jia, Cheng, Tangren, Zhang, Qixiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5293821/
https://www.ncbi.nlm.nih.gov/pubmed/28224001
http://dx.doi.org/10.3389/fpls.2017.00151
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author Bao, Fei
Du, Dongliang
An, Yang
Yang, Weiru
Wang, Jia
Cheng, Tangren
Zhang, Qixiang
author_facet Bao, Fei
Du, Dongliang
An, Yang
Yang, Weiru
Wang, Jia
Cheng, Tangren
Zhang, Qixiang
author_sort Bao, Fei
collection PubMed
description Dehydrins, known as group 2 or D-11 family late-embryogenesis-abundant (LEA) proteins, play important roles in plant growth and stress tolerance. Six dehydrin genes were previously identified from the genome of Prunus mume. In this study, five of them (PmLEA8, PmLEA10, PmLEA19, PmLEA20, and PmLEA29) were cloned from cold-resistant P. mume ‘Beijingyudie’. Real-time RT-PCR analysis indicated that all these genes could be up-regulated by one or several treatments (ABA, SA, low temperature, high temperature, PEG, and NaCl treatments). The results of spot assay demonstrated that the expression of all these dehydrins, except PmLEA8, conferred improved osmotic and freezing-resistance to the recombinant Escherichia coli. So four dehydrin genes, PmLEA10, PmLEA19, PmLEA20 and PmLEA29 were chosen for individual over-expression in tobacco plants. The transgenic tobacco plants showed lower relative content of malondialdehyde, relative electrolyte leakage and higher relative content of water than control plants when exposed to cold and drought stress. These results demonstrated that PmLEAs were involved in plant responses to cold and drought.
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spelling pubmed-52938212017-02-21 Overexpression of Prunus mume Dehydrin Genes in Tobacco Enhances Tolerance to Cold and Drought Bao, Fei Du, Dongliang An, Yang Yang, Weiru Wang, Jia Cheng, Tangren Zhang, Qixiang Front Plant Sci Plant Science Dehydrins, known as group 2 or D-11 family late-embryogenesis-abundant (LEA) proteins, play important roles in plant growth and stress tolerance. Six dehydrin genes were previously identified from the genome of Prunus mume. In this study, five of them (PmLEA8, PmLEA10, PmLEA19, PmLEA20, and PmLEA29) were cloned from cold-resistant P. mume ‘Beijingyudie’. Real-time RT-PCR analysis indicated that all these genes could be up-regulated by one or several treatments (ABA, SA, low temperature, high temperature, PEG, and NaCl treatments). The results of spot assay demonstrated that the expression of all these dehydrins, except PmLEA8, conferred improved osmotic and freezing-resistance to the recombinant Escherichia coli. So four dehydrin genes, PmLEA10, PmLEA19, PmLEA20 and PmLEA29 were chosen for individual over-expression in tobacco plants. The transgenic tobacco plants showed lower relative content of malondialdehyde, relative electrolyte leakage and higher relative content of water than control plants when exposed to cold and drought stress. These results demonstrated that PmLEAs were involved in plant responses to cold and drought. Frontiers Media S.A. 2017-02-07 /pmc/articles/PMC5293821/ /pubmed/28224001 http://dx.doi.org/10.3389/fpls.2017.00151 Text en Copyright © 2017 Bao, Du, An, Yang, Wang, Cheng and Zhang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Bao, Fei
Du, Dongliang
An, Yang
Yang, Weiru
Wang, Jia
Cheng, Tangren
Zhang, Qixiang
Overexpression of Prunus mume Dehydrin Genes in Tobacco Enhances Tolerance to Cold and Drought
title Overexpression of Prunus mume Dehydrin Genes in Tobacco Enhances Tolerance to Cold and Drought
title_full Overexpression of Prunus mume Dehydrin Genes in Tobacco Enhances Tolerance to Cold and Drought
title_fullStr Overexpression of Prunus mume Dehydrin Genes in Tobacco Enhances Tolerance to Cold and Drought
title_full_unstemmed Overexpression of Prunus mume Dehydrin Genes in Tobacco Enhances Tolerance to Cold and Drought
title_short Overexpression of Prunus mume Dehydrin Genes in Tobacco Enhances Tolerance to Cold and Drought
title_sort overexpression of prunus mume dehydrin genes in tobacco enhances tolerance to cold and drought
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5293821/
https://www.ncbi.nlm.nih.gov/pubmed/28224001
http://dx.doi.org/10.3389/fpls.2017.00151
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