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Bis(3,5-diiodo-2,4,6-trihydroxyphenyl)squaraine photodynamic therapy disrupts redox homeostasis and induce mitochondria-mediated apoptosis in human breast cancer cells

Photodynamic therapy (PDT) is a clinically established and highly evolving treatment modality for cancer. PDT utilizes a light responsive drug called photosensitizer that selectively destroys tumor cells upon light irradiation. Squaraines are a class of dyes possessing all favorable characteristics...

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Autores principales: Saneesh Babu, P. S., Manu, Prasad M., Dhanya, T. Jayaram, Tapas, Pradhan, Meera, R. Nair, Surendran, Arun, Aneesh, Kumar A., Vadakkancheril, S. Jisha, Ramaiah, Danaboyina, Nair, S. Asha, Pillai, M. Radhakrishna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5294812/
https://www.ncbi.nlm.nih.gov/pubmed/28169351
http://dx.doi.org/10.1038/srep42126
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author Saneesh Babu, P. S.
Manu, Prasad M.
Dhanya, T. Jayaram
Tapas, Pradhan
Meera, R. Nair
Surendran, Arun
Aneesh, Kumar A.
Vadakkancheril, S. Jisha
Ramaiah, Danaboyina
Nair, S. Asha
Pillai, M. Radhakrishna
author_facet Saneesh Babu, P. S.
Manu, Prasad M.
Dhanya, T. Jayaram
Tapas, Pradhan
Meera, R. Nair
Surendran, Arun
Aneesh, Kumar A.
Vadakkancheril, S. Jisha
Ramaiah, Danaboyina
Nair, S. Asha
Pillai, M. Radhakrishna
author_sort Saneesh Babu, P. S.
collection PubMed
description Photodynamic therapy (PDT) is a clinically established and highly evolving treatment modality for cancer. PDT utilizes a light responsive drug called photosensitizer that selectively destroys tumor cells upon light irradiation. Squaraines are a class of dyes possessing all favorable characteristics of a photosensitizer and have been considered to be a potent candidate for next generation PDT. In this study we chose an iodo derivative of squaraine called diiodo-squaraine (bis(3, 5-diiodo-2,4,6-trihydroxyphenyl)squaraine) which has been reported for its tumor specificity but least studied for its cellular and molecular functions. Our studies revealed that the iodo derivative of squaraine possess maximum photodynamic activity in human breast cancer cells MDA- MB- 231 and had very little cytotoxicity in normal breast cells MCF-10A. We analyzed its pro and anti-apoptotic events initiated by oxidative stress exploring a proteomic approach and delineated other critical molecular pathways and key proteins involved in regulating the complex network of cellular response upon PDT. Our study showed that, diiodo- squaraines predominantly accumulate in mitochondria and induce mitochondria-mediated apoptosis. Our study also reveals the novel mechanistic role of diiodo-squaraines to induce oxidative stress there by activating both protective and death inducing pathways post PDT.
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spelling pubmed-52948122017-02-10 Bis(3,5-diiodo-2,4,6-trihydroxyphenyl)squaraine photodynamic therapy disrupts redox homeostasis and induce mitochondria-mediated apoptosis in human breast cancer cells Saneesh Babu, P. S. Manu, Prasad M. Dhanya, T. Jayaram Tapas, Pradhan Meera, R. Nair Surendran, Arun Aneesh, Kumar A. Vadakkancheril, S. Jisha Ramaiah, Danaboyina Nair, S. Asha Pillai, M. Radhakrishna Sci Rep Article Photodynamic therapy (PDT) is a clinically established and highly evolving treatment modality for cancer. PDT utilizes a light responsive drug called photosensitizer that selectively destroys tumor cells upon light irradiation. Squaraines are a class of dyes possessing all favorable characteristics of a photosensitizer and have been considered to be a potent candidate for next generation PDT. In this study we chose an iodo derivative of squaraine called diiodo-squaraine (bis(3, 5-diiodo-2,4,6-trihydroxyphenyl)squaraine) which has been reported for its tumor specificity but least studied for its cellular and molecular functions. Our studies revealed that the iodo derivative of squaraine possess maximum photodynamic activity in human breast cancer cells MDA- MB- 231 and had very little cytotoxicity in normal breast cells MCF-10A. We analyzed its pro and anti-apoptotic events initiated by oxidative stress exploring a proteomic approach and delineated other critical molecular pathways and key proteins involved in regulating the complex network of cellular response upon PDT. Our study showed that, diiodo- squaraines predominantly accumulate in mitochondria and induce mitochondria-mediated apoptosis. Our study also reveals the novel mechanistic role of diiodo-squaraines to induce oxidative stress there by activating both protective and death inducing pathways post PDT. Nature Publishing Group 2017-02-07 /pmc/articles/PMC5294812/ /pubmed/28169351 http://dx.doi.org/10.1038/srep42126 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Saneesh Babu, P. S.
Manu, Prasad M.
Dhanya, T. Jayaram
Tapas, Pradhan
Meera, R. Nair
Surendran, Arun
Aneesh, Kumar A.
Vadakkancheril, S. Jisha
Ramaiah, Danaboyina
Nair, S. Asha
Pillai, M. Radhakrishna
Bis(3,5-diiodo-2,4,6-trihydroxyphenyl)squaraine photodynamic therapy disrupts redox homeostasis and induce mitochondria-mediated apoptosis in human breast cancer cells
title Bis(3,5-diiodo-2,4,6-trihydroxyphenyl)squaraine photodynamic therapy disrupts redox homeostasis and induce mitochondria-mediated apoptosis in human breast cancer cells
title_full Bis(3,5-diiodo-2,4,6-trihydroxyphenyl)squaraine photodynamic therapy disrupts redox homeostasis and induce mitochondria-mediated apoptosis in human breast cancer cells
title_fullStr Bis(3,5-diiodo-2,4,6-trihydroxyphenyl)squaraine photodynamic therapy disrupts redox homeostasis and induce mitochondria-mediated apoptosis in human breast cancer cells
title_full_unstemmed Bis(3,5-diiodo-2,4,6-trihydroxyphenyl)squaraine photodynamic therapy disrupts redox homeostasis and induce mitochondria-mediated apoptosis in human breast cancer cells
title_short Bis(3,5-diiodo-2,4,6-trihydroxyphenyl)squaraine photodynamic therapy disrupts redox homeostasis and induce mitochondria-mediated apoptosis in human breast cancer cells
title_sort bis(3,5-diiodo-2,4,6-trihydroxyphenyl)squaraine photodynamic therapy disrupts redox homeostasis and induce mitochondria-mediated apoptosis in human breast cancer cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5294812/
https://www.ncbi.nlm.nih.gov/pubmed/28169351
http://dx.doi.org/10.1038/srep42126
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