Cargando…
Contribution of the RgfD Quorum Sensing Peptide to rgf Regulation and Host Cell Association in Group B Streptococcus
Streptococcus agalactiae (group B Streptococcus; GBS) is a common inhabitant of the genitourinary and/or gastrointestinal tract in up to 40% of healthy adults; however, this opportunistic pathogen is able to breach restrictive host barriers to cause disease and persist in harsh and changing conditio...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5295018/ https://www.ncbi.nlm.nih.gov/pubmed/28067833 http://dx.doi.org/10.3390/genes8010023 |
_version_ | 1782505347830775808 |
---|---|
author | Parker, Robert E. Knupp, David Al Safadi, Rim Rosenau, Agnѐs Manning, Shannon D. |
author_facet | Parker, Robert E. Knupp, David Al Safadi, Rim Rosenau, Agnѐs Manning, Shannon D. |
author_sort | Parker, Robert E. |
collection | PubMed |
description | Streptococcus agalactiae (group B Streptococcus; GBS) is a common inhabitant of the genitourinary and/or gastrointestinal tract in up to 40% of healthy adults; however, this opportunistic pathogen is able to breach restrictive host barriers to cause disease and persist in harsh and changing conditions. This study sought to identify a role for quorum sensing, a form of cell to cell communication, in the regulation of the fibrinogen-binding (rgfBDAC) two-component system and the ability to associate with decidualized endometrial cells in vitro. To do this, we created a deletion in rgfD, which encodes the putative autoinducing peptide, in a GBS strain belonging to multilocus sequence type (ST)-17 and made comparisons to the wild type. Sequence variation in the rgf operon was detected in 40 clinical strains and a non-synonymous single nucleotide polymorphism was detected in rgfD in all of the ST-17 genomes that resulted in a truncation. Using qPCR, expression of rgf operon genes was significantly decreased in the ST-17 ΔrgfD mutant during exponential growth with the biggest difference (3.3-fold) occurring at higher cell densities. Association with decidualized endometrial cells was decreased 1.3-fold in the mutant relative to the wild type and rgfC expression was reduced 22-fold in ΔrgfD following exposure to the endometrial cells. Collectively, these data suggest that this putative quorum sensing molecule is important for attachment to human tissues and demonstrate a role for RgfD in GBS pathogenesis through regulation of rgfC. |
format | Online Article Text |
id | pubmed-5295018 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-52950182017-02-10 Contribution of the RgfD Quorum Sensing Peptide to rgf Regulation and Host Cell Association in Group B Streptococcus Parker, Robert E. Knupp, David Al Safadi, Rim Rosenau, Agnѐs Manning, Shannon D. Genes (Basel) Article Streptococcus agalactiae (group B Streptococcus; GBS) is a common inhabitant of the genitourinary and/or gastrointestinal tract in up to 40% of healthy adults; however, this opportunistic pathogen is able to breach restrictive host barriers to cause disease and persist in harsh and changing conditions. This study sought to identify a role for quorum sensing, a form of cell to cell communication, in the regulation of the fibrinogen-binding (rgfBDAC) two-component system and the ability to associate with decidualized endometrial cells in vitro. To do this, we created a deletion in rgfD, which encodes the putative autoinducing peptide, in a GBS strain belonging to multilocus sequence type (ST)-17 and made comparisons to the wild type. Sequence variation in the rgf operon was detected in 40 clinical strains and a non-synonymous single nucleotide polymorphism was detected in rgfD in all of the ST-17 genomes that resulted in a truncation. Using qPCR, expression of rgf operon genes was significantly decreased in the ST-17 ΔrgfD mutant during exponential growth with the biggest difference (3.3-fold) occurring at higher cell densities. Association with decidualized endometrial cells was decreased 1.3-fold in the mutant relative to the wild type and rgfC expression was reduced 22-fold in ΔrgfD following exposure to the endometrial cells. Collectively, these data suggest that this putative quorum sensing molecule is important for attachment to human tissues and demonstrate a role for RgfD in GBS pathogenesis through regulation of rgfC. MDPI 2017-01-06 /pmc/articles/PMC5295018/ /pubmed/28067833 http://dx.doi.org/10.3390/genes8010023 Text en © 2017 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Parker, Robert E. Knupp, David Al Safadi, Rim Rosenau, Agnѐs Manning, Shannon D. Contribution of the RgfD Quorum Sensing Peptide to rgf Regulation and Host Cell Association in Group B Streptococcus |
title | Contribution of the RgfD Quorum Sensing Peptide to rgf Regulation and Host Cell Association in Group B Streptococcus |
title_full | Contribution of the RgfD Quorum Sensing Peptide to rgf Regulation and Host Cell Association in Group B Streptococcus |
title_fullStr | Contribution of the RgfD Quorum Sensing Peptide to rgf Regulation and Host Cell Association in Group B Streptococcus |
title_full_unstemmed | Contribution of the RgfD Quorum Sensing Peptide to rgf Regulation and Host Cell Association in Group B Streptococcus |
title_short | Contribution of the RgfD Quorum Sensing Peptide to rgf Regulation and Host Cell Association in Group B Streptococcus |
title_sort | contribution of the rgfd quorum sensing peptide to rgf regulation and host cell association in group b streptococcus |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5295018/ https://www.ncbi.nlm.nih.gov/pubmed/28067833 http://dx.doi.org/10.3390/genes8010023 |
work_keys_str_mv | AT parkerroberte contributionofthergfdquorumsensingpeptidetorgfregulationandhostcellassociationingroupbstreptococcus AT knuppdavid contributionofthergfdquorumsensingpeptidetorgfregulationandhostcellassociationingroupbstreptococcus AT alsafadirim contributionofthergfdquorumsensingpeptidetorgfregulationandhostcellassociationingroupbstreptococcus AT rosenauagnes contributionofthergfdquorumsensingpeptidetorgfregulationandhostcellassociationingroupbstreptococcus AT manningshannond contributionofthergfdquorumsensingpeptidetorgfregulationandhostcellassociationingroupbstreptococcus |