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Visible Light-Responsive Platinum-Containing Titania Nanoparticle-Mediated Photocatalysis Induces Nucleotide Insertion, Deletion and Substitution Mutations
Conventional photocatalysts are primarily stimulated using ultraviolet (UV) light to elicit reactive oxygen species and have wide applications in environmental and energy fields, including self-cleaning surfaces and sterilization. Because UV illumination is hazardous to humans, visible light-respons...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5295192/ https://www.ncbi.nlm.nih.gov/pubmed/28336836 http://dx.doi.org/10.3390/nano7010002 |
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author | Sun, Der-Shan Tseng, Yao-Hsuan Wu, Wen-Shiang Wong, Ming-Show Chang, Hsin-Hou |
author_facet | Sun, Der-Shan Tseng, Yao-Hsuan Wu, Wen-Shiang Wong, Ming-Show Chang, Hsin-Hou |
author_sort | Sun, Der-Shan |
collection | PubMed |
description | Conventional photocatalysts are primarily stimulated using ultraviolet (UV) light to elicit reactive oxygen species and have wide applications in environmental and energy fields, including self-cleaning surfaces and sterilization. Because UV illumination is hazardous to humans, visible light-responsive photocatalysts (VLRPs) were discovered and are now applied to increase photocatalysis. However, fundamental questions regarding the ability of VLRPs to trigger DNA mutations and the mutation types it elicits remain elusive. Here, through plasmid transformation and β-galactosidase α-complementation analyses, we observed that visible light-responsive platinum-containing titania (TiO(2)) nanoparticle (NP)-mediated photocatalysis considerably reduces the number of Escherichia coli transformants. This suggests that such photocatalytic reactions cause DNA damage. DNA sequencing results demonstrated that the DNA damage comprises three mutation types, namely nucleotide insertion, deletion and substitution; this is the first study to report the types of mutations occurring after photocatalysis by TiO(2)-VLRPs. Our results may facilitate the development and appropriate use of new-generation TiO(2) NPs for biomedical applications. |
format | Online Article Text |
id | pubmed-5295192 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-52951922017-03-21 Visible Light-Responsive Platinum-Containing Titania Nanoparticle-Mediated Photocatalysis Induces Nucleotide Insertion, Deletion and Substitution Mutations Sun, Der-Shan Tseng, Yao-Hsuan Wu, Wen-Shiang Wong, Ming-Show Chang, Hsin-Hou Nanomaterials (Basel) Article Conventional photocatalysts are primarily stimulated using ultraviolet (UV) light to elicit reactive oxygen species and have wide applications in environmental and energy fields, including self-cleaning surfaces and sterilization. Because UV illumination is hazardous to humans, visible light-responsive photocatalysts (VLRPs) were discovered and are now applied to increase photocatalysis. However, fundamental questions regarding the ability of VLRPs to trigger DNA mutations and the mutation types it elicits remain elusive. Here, through plasmid transformation and β-galactosidase α-complementation analyses, we observed that visible light-responsive platinum-containing titania (TiO(2)) nanoparticle (NP)-mediated photocatalysis considerably reduces the number of Escherichia coli transformants. This suggests that such photocatalytic reactions cause DNA damage. DNA sequencing results demonstrated that the DNA damage comprises three mutation types, namely nucleotide insertion, deletion and substitution; this is the first study to report the types of mutations occurring after photocatalysis by TiO(2)-VLRPs. Our results may facilitate the development and appropriate use of new-generation TiO(2) NPs for biomedical applications. MDPI 2016-12-28 /pmc/articles/PMC5295192/ /pubmed/28336836 http://dx.doi.org/10.3390/nano7010002 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Sun, Der-Shan Tseng, Yao-Hsuan Wu, Wen-Shiang Wong, Ming-Show Chang, Hsin-Hou Visible Light-Responsive Platinum-Containing Titania Nanoparticle-Mediated Photocatalysis Induces Nucleotide Insertion, Deletion and Substitution Mutations |
title | Visible Light-Responsive Platinum-Containing Titania Nanoparticle-Mediated Photocatalysis Induces Nucleotide Insertion, Deletion and Substitution Mutations |
title_full | Visible Light-Responsive Platinum-Containing Titania Nanoparticle-Mediated Photocatalysis Induces Nucleotide Insertion, Deletion and Substitution Mutations |
title_fullStr | Visible Light-Responsive Platinum-Containing Titania Nanoparticle-Mediated Photocatalysis Induces Nucleotide Insertion, Deletion and Substitution Mutations |
title_full_unstemmed | Visible Light-Responsive Platinum-Containing Titania Nanoparticle-Mediated Photocatalysis Induces Nucleotide Insertion, Deletion and Substitution Mutations |
title_short | Visible Light-Responsive Platinum-Containing Titania Nanoparticle-Mediated Photocatalysis Induces Nucleotide Insertion, Deletion and Substitution Mutations |
title_sort | visible light-responsive platinum-containing titania nanoparticle-mediated photocatalysis induces nucleotide insertion, deletion and substitution mutations |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5295192/ https://www.ncbi.nlm.nih.gov/pubmed/28336836 http://dx.doi.org/10.3390/nano7010002 |
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