Effects of Ambient Fine Particles PM(2.5) on Human HaCaT Cells

The current study was conducted to observe the effects of fine particulate matter (PM(2.5)) on human keratinocyte cell line (HaCaT) cells. The potential mechanism linking PM(2.5) and skin was explored. HaCaT cells were cultured and then accessed in plate with PM(2.5). Cell viability was tested by Cell Counting Kit-8. The mRNA and protein expression of Filaggrin, Loricrin, Involucrin, and Repetin were analyzed. The levels of Granulocyte-macrophage Colony Stimulating Factor, Thymic Stromal Lymphopoietin, Tumor Necrosis Factor-α, Interleukin-1α, and Interleukin-8 were detected in the supernatant of the HaCaT cell with enzyme-linked immunosorbent assay kits. Cell viability decreased with the increase in PM(2.5). Compared with the control group, the protein expression of Filaggrin, Repetin, Involucrin, and Loricrin showed different expression patterns in PM(2.5) treatment groups. The level of Tumor Necrosis Factor-α, Thymic Stromal Lymphopoietin, Interleukin-1α, and Interleukin-8 significantly increased in the cells treated with PM(2.5). Ambient PM(2.5) may increase the risk of eczema and other skin diseases. The relative mechanism may be associated with the impairment of the skin barrier and the elevation of inflammatory responses.