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Antioxidant Pre-Treatment Reduces the Toxic Effects of Oxalate on Renal Epithelial Cells in a Cell Culture Model of Urolithiasis

Urolithiasis is characterized by the formation and retention of solid crystals within the urinary tract. Kidney stones are mostly composed of calcium oxalate, which predominantly generates free radicals that are toxic to renal tubular cells. The aim of the study is to explore possible effects of ant...

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Autores principales: Kizivat, Tomislav, Smolić, Martina, Marić, Ivana, Tolušić Levak, Maja, Smolić, Robert, Bilić Čurčić, Ines, Kuna, Lucija, Mihaljević, Ivan, Včev, Aleksandar, Tucak-Zorić, Sandra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5295359/
https://www.ncbi.nlm.nih.gov/pubmed/28125004
http://dx.doi.org/10.3390/ijerph14010109
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author Kizivat, Tomislav
Smolić, Martina
Marić, Ivana
Tolušić Levak, Maja
Smolić, Robert
Bilić Čurčić, Ines
Kuna, Lucija
Mihaljević, Ivan
Včev, Aleksandar
Tucak-Zorić, Sandra
author_facet Kizivat, Tomislav
Smolić, Martina
Marić, Ivana
Tolušić Levak, Maja
Smolić, Robert
Bilić Čurčić, Ines
Kuna, Lucija
Mihaljević, Ivan
Včev, Aleksandar
Tucak-Zorić, Sandra
author_sort Kizivat, Tomislav
collection PubMed
description Urolithiasis is characterized by the formation and retention of solid crystals within the urinary tract. Kidney stones are mostly composed of calcium oxalate, which predominantly generates free radicals that are toxic to renal tubular cells. The aim of the study is to explore possible effects of antioxidant pre-treatment on inhibition of oxidative stress. Three cell lines were used as in vitro model of urolithiasis: MDCK I, MDCK II and LLC-PK1. Oxidative stress was induced by exposure of cells to sodium oxalate in concentration of 8 mM. In order to prevent oxidative stress, cells were pre-treated with three different concentrations of l-arginine and vitamin E. Oxidative stress was evaluated by determining the expression of superoxide dismutase (SOD), osteopontin (OPN), and by the concentration of glutathione (GSH). In all three cell lines, pre-treatment of antioxidants increased cell survival. Positive correlation of SOD and OPN expression as well as GSH concentration was observed in all groups of cells. Our results indicate that an antioxidant pre-treatment with l-arginine and vitamin E is able to hamper oxalate-induced oxidative stress in kidney epithelial cells and as such could play a role in prevention of urolithiasis.
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spelling pubmed-52953592017-02-07 Antioxidant Pre-Treatment Reduces the Toxic Effects of Oxalate on Renal Epithelial Cells in a Cell Culture Model of Urolithiasis Kizivat, Tomislav Smolić, Martina Marić, Ivana Tolušić Levak, Maja Smolić, Robert Bilić Čurčić, Ines Kuna, Lucija Mihaljević, Ivan Včev, Aleksandar Tucak-Zorić, Sandra Int J Environ Res Public Health Article Urolithiasis is characterized by the formation and retention of solid crystals within the urinary tract. Kidney stones are mostly composed of calcium oxalate, which predominantly generates free radicals that are toxic to renal tubular cells. The aim of the study is to explore possible effects of antioxidant pre-treatment on inhibition of oxidative stress. Three cell lines were used as in vitro model of urolithiasis: MDCK I, MDCK II and LLC-PK1. Oxidative stress was induced by exposure of cells to sodium oxalate in concentration of 8 mM. In order to prevent oxidative stress, cells were pre-treated with three different concentrations of l-arginine and vitamin E. Oxidative stress was evaluated by determining the expression of superoxide dismutase (SOD), osteopontin (OPN), and by the concentration of glutathione (GSH). In all three cell lines, pre-treatment of antioxidants increased cell survival. Positive correlation of SOD and OPN expression as well as GSH concentration was observed in all groups of cells. Our results indicate that an antioxidant pre-treatment with l-arginine and vitamin E is able to hamper oxalate-induced oxidative stress in kidney epithelial cells and as such could play a role in prevention of urolithiasis. MDPI 2017-01-23 2017-01 /pmc/articles/PMC5295359/ /pubmed/28125004 http://dx.doi.org/10.3390/ijerph14010109 Text en © 2017 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kizivat, Tomislav
Smolić, Martina
Marić, Ivana
Tolušić Levak, Maja
Smolić, Robert
Bilić Čurčić, Ines
Kuna, Lucija
Mihaljević, Ivan
Včev, Aleksandar
Tucak-Zorić, Sandra
Antioxidant Pre-Treatment Reduces the Toxic Effects of Oxalate on Renal Epithelial Cells in a Cell Culture Model of Urolithiasis
title Antioxidant Pre-Treatment Reduces the Toxic Effects of Oxalate on Renal Epithelial Cells in a Cell Culture Model of Urolithiasis
title_full Antioxidant Pre-Treatment Reduces the Toxic Effects of Oxalate on Renal Epithelial Cells in a Cell Culture Model of Urolithiasis
title_fullStr Antioxidant Pre-Treatment Reduces the Toxic Effects of Oxalate on Renal Epithelial Cells in a Cell Culture Model of Urolithiasis
title_full_unstemmed Antioxidant Pre-Treatment Reduces the Toxic Effects of Oxalate on Renal Epithelial Cells in a Cell Culture Model of Urolithiasis
title_short Antioxidant Pre-Treatment Reduces the Toxic Effects of Oxalate on Renal Epithelial Cells in a Cell Culture Model of Urolithiasis
title_sort antioxidant pre-treatment reduces the toxic effects of oxalate on renal epithelial cells in a cell culture model of urolithiasis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5295359/
https://www.ncbi.nlm.nih.gov/pubmed/28125004
http://dx.doi.org/10.3390/ijerph14010109
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