A perspective on conformational control of electron transfer in nitric oxide synthases

This perspective reviews single molecule and ensemble fluorescence spectroscopy studies of the three tissue specific nitric oxide synthase (NOS) isoenzymes and the related diflavin oxidoreductase cytochrome P450 reductase. The focus is on the role of protein dynamics and the protein conformational l...

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Detalles Bibliográficos
Autores principales: Hedison, Tobias M., Hay, Sam, Scrutton, Nigel S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5295631/
https://www.ncbi.nlm.nih.gov/pubmed/27619338
http://dx.doi.org/10.1016/j.niox.2016.09.002
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author Hedison, Tobias M.
Hay, Sam
Scrutton, Nigel S.
author_facet Hedison, Tobias M.
Hay, Sam
Scrutton, Nigel S.
author_sort Hedison, Tobias M.
collection PubMed
description This perspective reviews single molecule and ensemble fluorescence spectroscopy studies of the three tissue specific nitric oxide synthase (NOS) isoenzymes and the related diflavin oxidoreductase cytochrome P450 reductase. The focus is on the role of protein dynamics and the protein conformational landscape and we discuss how recent fluorescence-based studies have helped in illustrating how the nature of the NOS conformational landscape relates to enzyme turnover and catalysis.
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spelling pubmed-52956312017-02-28 A perspective on conformational control of electron transfer in nitric oxide synthases Hedison, Tobias M. Hay, Sam Scrutton, Nigel S. Nitric Oxide Article This perspective reviews single molecule and ensemble fluorescence spectroscopy studies of the three tissue specific nitric oxide synthase (NOS) isoenzymes and the related diflavin oxidoreductase cytochrome P450 reductase. The focus is on the role of protein dynamics and the protein conformational landscape and we discuss how recent fluorescence-based studies have helped in illustrating how the nature of the NOS conformational landscape relates to enzyme turnover and catalysis. Elsevier 2017-02-28 /pmc/articles/PMC5295631/ /pubmed/27619338 http://dx.doi.org/10.1016/j.niox.2016.09.002 Text en © 2016 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hedison, Tobias M.
Hay, Sam
Scrutton, Nigel S.
A perspective on conformational control of electron transfer in nitric oxide synthases
title A perspective on conformational control of electron transfer in nitric oxide synthases
title_full A perspective on conformational control of electron transfer in nitric oxide synthases
title_fullStr A perspective on conformational control of electron transfer in nitric oxide synthases
title_full_unstemmed A perspective on conformational control of electron transfer in nitric oxide synthases
title_short A perspective on conformational control of electron transfer in nitric oxide synthases
title_sort perspective on conformational control of electron transfer in nitric oxide synthases
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5295631/
https://www.ncbi.nlm.nih.gov/pubmed/27619338
http://dx.doi.org/10.1016/j.niox.2016.09.002
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