Cargando…
Gut microbial diversity analysis using Illumina sequencing for functional dyspepsia with liver depression-spleen deficiency syndrome and the interventional Xiaoyaosan in a rat model
AIM: To investigate gut microbial diversity and the interventional effect of Xiaoyaosan (XYS) in a rat model of functional dyspepsia (FD) with liver depression-spleen deficiency syndrome. METHODS: The FD with liver depression-spleen deficiency syndrome rat model was established through classic chron...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Baishideng Publishing Group Inc
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5296197/ https://www.ncbi.nlm.nih.gov/pubmed/28223725 http://dx.doi.org/10.3748/wjg.v23.i5.810 |
_version_ | 1782505567579799552 |
---|---|
author | Qiu, Juan-Juan Liu, Zhe Zhao, Peng Wang, Xue-Jun Li, Yu-Chun Sui, Hua Owusu, Lawrence Guo, Hui-Shu Cai, Zheng-Xu |
author_facet | Qiu, Juan-Juan Liu, Zhe Zhao, Peng Wang, Xue-Jun Li, Yu-Chun Sui, Hua Owusu, Lawrence Guo, Hui-Shu Cai, Zheng-Xu |
author_sort | Qiu, Juan-Juan |
collection | PubMed |
description | AIM: To investigate gut microbial diversity and the interventional effect of Xiaoyaosan (XYS) in a rat model of functional dyspepsia (FD) with liver depression-spleen deficiency syndrome. METHODS: The FD with liver depression-spleen deficiency syndrome rat model was established through classic chronic mild unpredictable stimulation every day. XYS group rats received XYS 1 h before the stimulation. The models were assessed by parameters including state of the rat, weight, sucrose test result and open-field test result. After 3 wk, the stools of rats were collected and genomic DNA was extracted. PCR products of the V4 region of 16S rDNA were sequenced using a barcoded Illumina paired-end sequencing technique. The primary composition of the microbiome in the stool samples was determined and analyzed by cluster analysis. RESULTS: Rat models were successfully established, per data from rat state, weight and open-field test. The microbiomes contained 20 phyla from all samples. Firmicutes, Bacteroidetes, Proteobacteria, Cyanobacteria and Tenericutes were the most abundant taxonomic groups. The relative abundance of Firmicutes, Proteobacteria and Cyanobacteria in the model group was higher than that in the normal group. On the contrary, the relative abundance of Bacteroidetes in the model group was lower than that in the normal group. Upon XYS treatment, the relative abundance of all dysregulated phyla was restored to levels similar to those observed in the normal group. Abundance clustering heat map of phyla corroborated the taxonomic distribution. CONCLUSION: The microbiome relative abundance of FD rats with liver depression-spleen deficiency syndrome was significantly different from the normal cohort. XYS intervention may effectively adjust the gut dysbacteriosis in FD. |
format | Online Article Text |
id | pubmed-5296197 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Baishideng Publishing Group Inc |
record_format | MEDLINE/PubMed |
spelling | pubmed-52961972017-02-21 Gut microbial diversity analysis using Illumina sequencing for functional dyspepsia with liver depression-spleen deficiency syndrome and the interventional Xiaoyaosan in a rat model Qiu, Juan-Juan Liu, Zhe Zhao, Peng Wang, Xue-Jun Li, Yu-Chun Sui, Hua Owusu, Lawrence Guo, Hui-Shu Cai, Zheng-Xu World J Gastroenterol Basic Study AIM: To investigate gut microbial diversity and the interventional effect of Xiaoyaosan (XYS) in a rat model of functional dyspepsia (FD) with liver depression-spleen deficiency syndrome. METHODS: The FD with liver depression-spleen deficiency syndrome rat model was established through classic chronic mild unpredictable stimulation every day. XYS group rats received XYS 1 h before the stimulation. The models were assessed by parameters including state of the rat, weight, sucrose test result and open-field test result. After 3 wk, the stools of rats were collected and genomic DNA was extracted. PCR products of the V4 region of 16S rDNA were sequenced using a barcoded Illumina paired-end sequencing technique. The primary composition of the microbiome in the stool samples was determined and analyzed by cluster analysis. RESULTS: Rat models were successfully established, per data from rat state, weight and open-field test. The microbiomes contained 20 phyla from all samples. Firmicutes, Bacteroidetes, Proteobacteria, Cyanobacteria and Tenericutes were the most abundant taxonomic groups. The relative abundance of Firmicutes, Proteobacteria and Cyanobacteria in the model group was higher than that in the normal group. On the contrary, the relative abundance of Bacteroidetes in the model group was lower than that in the normal group. Upon XYS treatment, the relative abundance of all dysregulated phyla was restored to levels similar to those observed in the normal group. Abundance clustering heat map of phyla corroborated the taxonomic distribution. CONCLUSION: The microbiome relative abundance of FD rats with liver depression-spleen deficiency syndrome was significantly different from the normal cohort. XYS intervention may effectively adjust the gut dysbacteriosis in FD. Baishideng Publishing Group Inc 2017-02-07 2017-02-07 /pmc/articles/PMC5296197/ /pubmed/28223725 http://dx.doi.org/10.3748/wjg.v23.i5.810 Text en ©The Author(s) 2017. Published by Baishideng Publishing Group Inc. All rights reserved. http://creativecommons.org/licenses/by-nc/4.0/ This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. |
spellingShingle | Basic Study Qiu, Juan-Juan Liu, Zhe Zhao, Peng Wang, Xue-Jun Li, Yu-Chun Sui, Hua Owusu, Lawrence Guo, Hui-Shu Cai, Zheng-Xu Gut microbial diversity analysis using Illumina sequencing for functional dyspepsia with liver depression-spleen deficiency syndrome and the interventional Xiaoyaosan in a rat model |
title | Gut microbial diversity analysis using Illumina sequencing for functional dyspepsia with liver depression-spleen deficiency syndrome and the interventional Xiaoyaosan in a rat model |
title_full | Gut microbial diversity analysis using Illumina sequencing for functional dyspepsia with liver depression-spleen deficiency syndrome and the interventional Xiaoyaosan in a rat model |
title_fullStr | Gut microbial diversity analysis using Illumina sequencing for functional dyspepsia with liver depression-spleen deficiency syndrome and the interventional Xiaoyaosan in a rat model |
title_full_unstemmed | Gut microbial diversity analysis using Illumina sequencing for functional dyspepsia with liver depression-spleen deficiency syndrome and the interventional Xiaoyaosan in a rat model |
title_short | Gut microbial diversity analysis using Illumina sequencing for functional dyspepsia with liver depression-spleen deficiency syndrome and the interventional Xiaoyaosan in a rat model |
title_sort | gut microbial diversity analysis using illumina sequencing for functional dyspepsia with liver depression-spleen deficiency syndrome and the interventional xiaoyaosan in a rat model |
topic | Basic Study |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5296197/ https://www.ncbi.nlm.nih.gov/pubmed/28223725 http://dx.doi.org/10.3748/wjg.v23.i5.810 |
work_keys_str_mv | AT qiujuanjuan gutmicrobialdiversityanalysisusingilluminasequencingforfunctionaldyspepsiawithliverdepressionspleendeficiencysyndromeandtheinterventionalxiaoyaosaninaratmodel AT liuzhe gutmicrobialdiversityanalysisusingilluminasequencingforfunctionaldyspepsiawithliverdepressionspleendeficiencysyndromeandtheinterventionalxiaoyaosaninaratmodel AT zhaopeng gutmicrobialdiversityanalysisusingilluminasequencingforfunctionaldyspepsiawithliverdepressionspleendeficiencysyndromeandtheinterventionalxiaoyaosaninaratmodel AT wangxuejun gutmicrobialdiversityanalysisusingilluminasequencingforfunctionaldyspepsiawithliverdepressionspleendeficiencysyndromeandtheinterventionalxiaoyaosaninaratmodel AT liyuchun gutmicrobialdiversityanalysisusingilluminasequencingforfunctionaldyspepsiawithliverdepressionspleendeficiencysyndromeandtheinterventionalxiaoyaosaninaratmodel AT suihua gutmicrobialdiversityanalysisusingilluminasequencingforfunctionaldyspepsiawithliverdepressionspleendeficiencysyndromeandtheinterventionalxiaoyaosaninaratmodel AT owusulawrence gutmicrobialdiversityanalysisusingilluminasequencingforfunctionaldyspepsiawithliverdepressionspleendeficiencysyndromeandtheinterventionalxiaoyaosaninaratmodel AT guohuishu gutmicrobialdiversityanalysisusingilluminasequencingforfunctionaldyspepsiawithliverdepressionspleendeficiencysyndromeandtheinterventionalxiaoyaosaninaratmodel AT caizhengxu gutmicrobialdiversityanalysisusingilluminasequencingforfunctionaldyspepsiawithliverdepressionspleendeficiencysyndromeandtheinterventionalxiaoyaosaninaratmodel |