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Identification of ZOUPI Orthologs in Soybean Potentially Involved in Endosperm Breakdown and Embryogenic Development

Soybean (Glycine max Merr.) is the world’s most widely grown legume and provides an important source of protein and oil. Improvement of seed quality requires deep insights into the genetic regulation of seed development. The endosperm serves as a temporary source of nutrients that are transported fr...

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Autores principales: Zhang, Yaohua, Li, Xin, Yang, Suxin, Feng, Xianzhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5296293/
https://www.ncbi.nlm.nih.gov/pubmed/28228767
http://dx.doi.org/10.3389/fpls.2017.00139
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author Zhang, Yaohua
Li, Xin
Yang, Suxin
Feng, Xianzhong
author_facet Zhang, Yaohua
Li, Xin
Yang, Suxin
Feng, Xianzhong
author_sort Zhang, Yaohua
collection PubMed
description Soybean (Glycine max Merr.) is the world’s most widely grown legume and provides an important source of protein and oil. Improvement of seed quality requires deep insights into the genetic regulation of seed development. The endosperm serves as a temporary source of nutrients that are transported from maternal to filial tissues, and it also generates signals for proper embryo formation. Endosperm cell death is associated with the processes of nutrient transfer and embryo expansion. The bHLH domain transcription factor AtZHOUPI (AtZOU) plays a key role in both the lysis of the transient endosperm and the formation of embryo cuticle in Arabidopsis thaliana. There are two copies of soybean GmZOU (GmZOU-1 and GmZOU-2), which fall into the same phylogenetic clade as AtZOU. These two copies share the same transcription orientation and are the result of tandem duplication. The expression of GmZOUs is limited to the endosperm, where it peaks during the heart embryo stage. When the exogenous GmZOU-1 and GmZOU-2 were expressed in the zou-4 mutant of Arabidopsis, only GmZOU-1 partially complemented the zou mutant phenotype, as indicated by endosperm breakdown and embryo cuticle formation in the transgenic lines. This research confirmed that the GmZOU-1 is a ZOU ortholog that may be responsible for endosperm breakdown and embryo cuticle formation in soybean.
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spelling pubmed-52962932017-02-22 Identification of ZOUPI Orthologs in Soybean Potentially Involved in Endosperm Breakdown and Embryogenic Development Zhang, Yaohua Li, Xin Yang, Suxin Feng, Xianzhong Front Plant Sci Plant Science Soybean (Glycine max Merr.) is the world’s most widely grown legume and provides an important source of protein and oil. Improvement of seed quality requires deep insights into the genetic regulation of seed development. The endosperm serves as a temporary source of nutrients that are transported from maternal to filial tissues, and it also generates signals for proper embryo formation. Endosperm cell death is associated with the processes of nutrient transfer and embryo expansion. The bHLH domain transcription factor AtZHOUPI (AtZOU) plays a key role in both the lysis of the transient endosperm and the formation of embryo cuticle in Arabidopsis thaliana. There are two copies of soybean GmZOU (GmZOU-1 and GmZOU-2), which fall into the same phylogenetic clade as AtZOU. These two copies share the same transcription orientation and are the result of tandem duplication. The expression of GmZOUs is limited to the endosperm, where it peaks during the heart embryo stage. When the exogenous GmZOU-1 and GmZOU-2 were expressed in the zou-4 mutant of Arabidopsis, only GmZOU-1 partially complemented the zou mutant phenotype, as indicated by endosperm breakdown and embryo cuticle formation in the transgenic lines. This research confirmed that the GmZOU-1 is a ZOU ortholog that may be responsible for endosperm breakdown and embryo cuticle formation in soybean. Frontiers Media S.A. 2017-02-08 /pmc/articles/PMC5296293/ /pubmed/28228767 http://dx.doi.org/10.3389/fpls.2017.00139 Text en Copyright © 2017 Zhang, Li, Yang and Feng. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Zhang, Yaohua
Li, Xin
Yang, Suxin
Feng, Xianzhong
Identification of ZOUPI Orthologs in Soybean Potentially Involved in Endosperm Breakdown and Embryogenic Development
title Identification of ZOUPI Orthologs in Soybean Potentially Involved in Endosperm Breakdown and Embryogenic Development
title_full Identification of ZOUPI Orthologs in Soybean Potentially Involved in Endosperm Breakdown and Embryogenic Development
title_fullStr Identification of ZOUPI Orthologs in Soybean Potentially Involved in Endosperm Breakdown and Embryogenic Development
title_full_unstemmed Identification of ZOUPI Orthologs in Soybean Potentially Involved in Endosperm Breakdown and Embryogenic Development
title_short Identification of ZOUPI Orthologs in Soybean Potentially Involved in Endosperm Breakdown and Embryogenic Development
title_sort identification of zoupi orthologs in soybean potentially involved in endosperm breakdown and embryogenic development
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5296293/
https://www.ncbi.nlm.nih.gov/pubmed/28228767
http://dx.doi.org/10.3389/fpls.2017.00139
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