A Simple and Rapid Method for Quality Control of Major Histocompatibility Complex–Peptide Monomers by Flow Cytometry

Major histocompatibility complex (MHC) multimers are essential tools in T cell immunomonitoring, which are employed both in basic and clinical research, as well as for assessing clinical samples during therapy. The generation of MHC monomers loaded with synthetic peptides is an elaborate and time-co...

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Autores principales: Chandran, P. Anoop, Heidu, Sonja, Zelba, Henning, Schmid-Horch, Barbara, Rammensee, Hans-Georg, Pascolo, Steve, Gouttefangeas, Cécile
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Immunology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5296342/
https://www.ncbi.nlm.nih.gov/pubmed/28228758
http://dx.doi.org/10.3389/fimmu.2017.00096
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author Chandran, P. Anoop
Heidu, Sonja
Zelba, Henning
Schmid-Horch, Barbara
Rammensee, Hans-Georg
Pascolo, Steve
Gouttefangeas, Cécile
author_facet Chandran, P. Anoop
Heidu, Sonja
Zelba, Henning
Schmid-Horch, Barbara
Rammensee, Hans-Georg
Pascolo, Steve
Gouttefangeas, Cécile
author_sort Chandran, P. Anoop
collection PubMed
description Major histocompatibility complex (MHC) multimers are essential tools in T cell immunomonitoring, which are employed both in basic and clinical research, as well as for assessing clinical samples during therapy. The generation of MHC monomers loaded with synthetic peptides is an elaborate and time-consuming process. It would be beneficial to assess the quality of these monomers prior to downstream applications. In this technical note, we describe a novel flow cytometry-based, cell-free, quick, and robust assay to check the quality of MHC monomers directly after refolding or after long-term storage.
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spelling pubmed-52963422017-02-22 A Simple and Rapid Method for Quality Control of Major Histocompatibility Complex–Peptide Monomers by Flow Cytometry Chandran, P. Anoop Heidu, Sonja Zelba, Henning Schmid-Horch, Barbara Rammensee, Hans-Georg Pascolo, Steve Gouttefangeas, Cécile Front Immunol Immunology Major histocompatibility complex (MHC) multimers are essential tools in T cell immunomonitoring, which are employed both in basic and clinical research, as well as for assessing clinical samples during therapy. The generation of MHC monomers loaded with synthetic peptides is an elaborate and time-consuming process. It would be beneficial to assess the quality of these monomers prior to downstream applications. In this technical note, we describe a novel flow cytometry-based, cell-free, quick, and robust assay to check the quality of MHC monomers directly after refolding or after long-term storage. Frontiers Media S.A. 2017-02-08 /pmc/articles/PMC5296342/ /pubmed/28228758 http://dx.doi.org/10.3389/fimmu.2017.00096 Text en Copyright © 2017 Chandran, Heidu, Zelba, Schmid-Horch, Rammensee, Pascolo and Gouttefangeas. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Chandran, P. Anoop
Heidu, Sonja
Zelba, Henning
Schmid-Horch, Barbara
Rammensee, Hans-Georg
Pascolo, Steve
Gouttefangeas, Cécile
A Simple and Rapid Method for Quality Control of Major Histocompatibility Complex–Peptide Monomers by Flow Cytometry
title A Simple and Rapid Method for Quality Control of Major Histocompatibility Complex–Peptide Monomers by Flow Cytometry
title_full A Simple and Rapid Method for Quality Control of Major Histocompatibility Complex–Peptide Monomers by Flow Cytometry
title_fullStr A Simple and Rapid Method for Quality Control of Major Histocompatibility Complex–Peptide Monomers by Flow Cytometry
title_full_unstemmed A Simple and Rapid Method for Quality Control of Major Histocompatibility Complex–Peptide Monomers by Flow Cytometry
title_short A Simple and Rapid Method for Quality Control of Major Histocompatibility Complex–Peptide Monomers by Flow Cytometry
title_sort simple and rapid method for quality control of major histocompatibility complex–peptide monomers by flow cytometry
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5296342/
https://www.ncbi.nlm.nih.gov/pubmed/28228758
http://dx.doi.org/10.3389/fimmu.2017.00096
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