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Primary macrophages and J774 cells respond differently to infection with Mycobacterium tuberculosis

Macrophages play an essential role in the early immune response to Mycobacterium tuberculosis and are the cell type preferentially infected in vivo. Primary macrophages and macrophage-like cell lines are commonly used as infection models, although the physiological relevance of cell lines, particula...

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Autores principales: Andreu, Nuria, Phelan, Jody, de Sessions, Paola F., Cliff, Jacqueline M., Clark, Taane G., Hibberd, Martin L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5296737/
https://www.ncbi.nlm.nih.gov/pubmed/28176867
http://dx.doi.org/10.1038/srep42225
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author Andreu, Nuria
Phelan, Jody
de Sessions, Paola F.
Cliff, Jacqueline M.
Clark, Taane G.
Hibberd, Martin L.
author_facet Andreu, Nuria
Phelan, Jody
de Sessions, Paola F.
Cliff, Jacqueline M.
Clark, Taane G.
Hibberd, Martin L.
author_sort Andreu, Nuria
collection PubMed
description Macrophages play an essential role in the early immune response to Mycobacterium tuberculosis and are the cell type preferentially infected in vivo. Primary macrophages and macrophage-like cell lines are commonly used as infection models, although the physiological relevance of cell lines, particularly for host-pathogen interaction studies, is debatable. Here we use high-throughput RNA-sequencing to analyse transcriptome dynamics of two macrophage models in response to M. tuberculosis infection. Specifically, we study the early response of bone marrow-derived mouse macrophages and cell line J774 to infection with live and γ-irradiated (killed) M. tuberculosis. We show that infection with live bacilli specifically alters the expression of host genes such as Rsad2, Ifit1/2/3 and Rig-I, whose potential roles in resistance to M. tuberculosis infection have not yet been investigated. In addition, the response of primary macrophages is faster and more intense than that of J774 cells in terms of number of differentially expressed genes and magnitude of induction/repression. Our results point to potentially novel processes leading to immune containment early during M. tuberculosis infection, and support the idea that important differences exist between primary macrophages and cell lines, which should be taken into account when choosing a macrophage model to study host-pathogen interactions.
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spelling pubmed-52967372017-02-10 Primary macrophages and J774 cells respond differently to infection with Mycobacterium tuberculosis Andreu, Nuria Phelan, Jody de Sessions, Paola F. Cliff, Jacqueline M. Clark, Taane G. Hibberd, Martin L. Sci Rep Article Macrophages play an essential role in the early immune response to Mycobacterium tuberculosis and are the cell type preferentially infected in vivo. Primary macrophages and macrophage-like cell lines are commonly used as infection models, although the physiological relevance of cell lines, particularly for host-pathogen interaction studies, is debatable. Here we use high-throughput RNA-sequencing to analyse transcriptome dynamics of two macrophage models in response to M. tuberculosis infection. Specifically, we study the early response of bone marrow-derived mouse macrophages and cell line J774 to infection with live and γ-irradiated (killed) M. tuberculosis. We show that infection with live bacilli specifically alters the expression of host genes such as Rsad2, Ifit1/2/3 and Rig-I, whose potential roles in resistance to M. tuberculosis infection have not yet been investigated. In addition, the response of primary macrophages is faster and more intense than that of J774 cells in terms of number of differentially expressed genes and magnitude of induction/repression. Our results point to potentially novel processes leading to immune containment early during M. tuberculosis infection, and support the idea that important differences exist between primary macrophages and cell lines, which should be taken into account when choosing a macrophage model to study host-pathogen interactions. Nature Publishing Group 2017-02-08 /pmc/articles/PMC5296737/ /pubmed/28176867 http://dx.doi.org/10.1038/srep42225 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Andreu, Nuria
Phelan, Jody
de Sessions, Paola F.
Cliff, Jacqueline M.
Clark, Taane G.
Hibberd, Martin L.
Primary macrophages and J774 cells respond differently to infection with Mycobacterium tuberculosis
title Primary macrophages and J774 cells respond differently to infection with Mycobacterium tuberculosis
title_full Primary macrophages and J774 cells respond differently to infection with Mycobacterium tuberculosis
title_fullStr Primary macrophages and J774 cells respond differently to infection with Mycobacterium tuberculosis
title_full_unstemmed Primary macrophages and J774 cells respond differently to infection with Mycobacterium tuberculosis
title_short Primary macrophages and J774 cells respond differently to infection with Mycobacterium tuberculosis
title_sort primary macrophages and j774 cells respond differently to infection with mycobacterium tuberculosis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5296737/
https://www.ncbi.nlm.nih.gov/pubmed/28176867
http://dx.doi.org/10.1038/srep42225
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