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CRISPR/Cas9-mediated gene manipulation to create single-amino-acid-substituted and floxed mice with a cloning-free method

Clustered regulatory interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) technology is a powerful tool to manipulate the genome with extraordinary simplicity and speed. To generate genetically modified animals, CRISPR/Cas9-mediated genome editing is typically accomplish...

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Detalles Bibliográficos
Autores principales:	Ma, Xiaolong, Chen, Chao, Veevers, Jennifer, Zhou, XinMin, Ross, Robert S., Feng, Wei, Chen, Ju
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group 2017
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5296764/ https://www.ncbi.nlm.nih.gov/pubmed/28176880 http://dx.doi.org/10.1038/srep42244

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