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Anti-Tumor Effects of Atractylenolide-I on Human Ovarian Cancer Cells
BACKGROUND: The aim of this study was to investigate the effects of Atractylenolide-I (AT-I), a naturally occurring sesquiterpene lactone isolated from Atractylodes macrocephala Koidz, on human ovarian cancer cells. MATERIAL/METHODS: The viability and anchorage-independent growth of ovarian cancer c...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
International Scientific Literature, Inc.
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5297331/ https://www.ncbi.nlm.nih.gov/pubmed/28141785 http://dx.doi.org/10.12659/MSM.902886 |
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author | Long, Fangyi Wang, Ting Jia, Ping Wang, Huafei Qing, Yi Xiong, Tingting He, Mengjie Wang, Xiaoli |
author_facet | Long, Fangyi Wang, Ting Jia, Ping Wang, Huafei Qing, Yi Xiong, Tingting He, Mengjie Wang, Xiaoli |
author_sort | Long, Fangyi |
collection | PubMed |
description | BACKGROUND: The aim of this study was to investigate the effects of Atractylenolide-I (AT-I), a naturally occurring sesquiterpene lactone isolated from Atractylodes macrocephala Koidz, on human ovarian cancer cells. MATERIAL/METHODS: The viability and anchorage-independent growth of ovarian cancer cells were evaluated using MTT and colony formation assay, respectively. Cell cycle and apoptosis were detected with flow cytometry analysis. The level of cyclin B1 and CDK1 was measured using qPCR and ELISA analysis. The expression of Bax, cleaved caspase-9, cleaved caspase-3, cytochrome c, AIF, and Bcl-2, and phosphorylation level of PI3K, AKT, and mTOR were determined with Western blot analysis. RESULTS: AT-I decreased the cell viability and suppressed anchorage-independent growth of A2780 cells. Cell cycle was arrested in G2/M phase transition by AT-I treatment, which was related to decreased expression of cyclin B1 and CDK1 in a dose-dependent manner. In addition, the treatment induced apoptosis, as shown by up-regulation of Bax, cleaved caspase-9, cleaved caspase-3, and cytosolic release of cytochrome c and AIF, and down-regulation of Bcl-2, in a dose-dependent manner. Then, the effects of AT-I on PI3K/Akt/mTOR pathways were examined to further investigate the underlying anti-cancer mechanism of AT-I, and the results showed that treatment with AT-I significantly decreased the phosphorylation level of PI3K, Akt, and mTOR. CONCLUSIONS: This study demonstrated that AT-I induced cell cycle arrest and apoptosis through inhibition of PI3K/Akt/mTOR pathway in ovarian cancer cells. These results suggest that AT-I might be a potential therapeutic agent in the treatment of ovarian cancer. |
format | Online Article Text |
id | pubmed-5297331 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | International Scientific Literature, Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-52973312017-02-08 Anti-Tumor Effects of Atractylenolide-I on Human Ovarian Cancer Cells Long, Fangyi Wang, Ting Jia, Ping Wang, Huafei Qing, Yi Xiong, Tingting He, Mengjie Wang, Xiaoli Med Sci Monit Lab/In Vitro Research BACKGROUND: The aim of this study was to investigate the effects of Atractylenolide-I (AT-I), a naturally occurring sesquiterpene lactone isolated from Atractylodes macrocephala Koidz, on human ovarian cancer cells. MATERIAL/METHODS: The viability and anchorage-independent growth of ovarian cancer cells were evaluated using MTT and colony formation assay, respectively. Cell cycle and apoptosis were detected with flow cytometry analysis. The level of cyclin B1 and CDK1 was measured using qPCR and ELISA analysis. The expression of Bax, cleaved caspase-9, cleaved caspase-3, cytochrome c, AIF, and Bcl-2, and phosphorylation level of PI3K, AKT, and mTOR were determined with Western blot analysis. RESULTS: AT-I decreased the cell viability and suppressed anchorage-independent growth of A2780 cells. Cell cycle was arrested in G2/M phase transition by AT-I treatment, which was related to decreased expression of cyclin B1 and CDK1 in a dose-dependent manner. In addition, the treatment induced apoptosis, as shown by up-regulation of Bax, cleaved caspase-9, cleaved caspase-3, and cytosolic release of cytochrome c and AIF, and down-regulation of Bcl-2, in a dose-dependent manner. Then, the effects of AT-I on PI3K/Akt/mTOR pathways were examined to further investigate the underlying anti-cancer mechanism of AT-I, and the results showed that treatment with AT-I significantly decreased the phosphorylation level of PI3K, Akt, and mTOR. CONCLUSIONS: This study demonstrated that AT-I induced cell cycle arrest and apoptosis through inhibition of PI3K/Akt/mTOR pathway in ovarian cancer cells. These results suggest that AT-I might be a potential therapeutic agent in the treatment of ovarian cancer. International Scientific Literature, Inc. 2017-01-31 /pmc/articles/PMC5297331/ /pubmed/28141785 http://dx.doi.org/10.12659/MSM.902886 Text en © Med Sci Monit, 2017 This work is licensed under Creative Common Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) |
spellingShingle | Lab/In Vitro Research Long, Fangyi Wang, Ting Jia, Ping Wang, Huafei Qing, Yi Xiong, Tingting He, Mengjie Wang, Xiaoli Anti-Tumor Effects of Atractylenolide-I on Human Ovarian Cancer Cells |
title | Anti-Tumor Effects of Atractylenolide-I on Human Ovarian Cancer Cells |
title_full | Anti-Tumor Effects of Atractylenolide-I on Human Ovarian Cancer Cells |
title_fullStr | Anti-Tumor Effects of Atractylenolide-I on Human Ovarian Cancer Cells |
title_full_unstemmed | Anti-Tumor Effects of Atractylenolide-I on Human Ovarian Cancer Cells |
title_short | Anti-Tumor Effects of Atractylenolide-I on Human Ovarian Cancer Cells |
title_sort | anti-tumor effects of atractylenolide-i on human ovarian cancer cells |
topic | Lab/In Vitro Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5297331/ https://www.ncbi.nlm.nih.gov/pubmed/28141785 http://dx.doi.org/10.12659/MSM.902886 |
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