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Influence of Anode Potentials on Current Generation and Extracellular Electron Transfer Paths of Geobacter Species

Geobacter species are capable of utilizing solid-state compounds, including anodic electrodes, as electron acceptors of respiration via extracellular electron transfer (EET) and have attracted considerable attention for their crucial role as biocatalysts of bioelectrochemical systems (BES’s). Recent...

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Autor principal: Kato, Souichiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5297742/
https://www.ncbi.nlm.nih.gov/pubmed/28067820
http://dx.doi.org/10.3390/ijms18010108
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author Kato, Souichiro
author_facet Kato, Souichiro
author_sort Kato, Souichiro
collection PubMed
description Geobacter species are capable of utilizing solid-state compounds, including anodic electrodes, as electron acceptors of respiration via extracellular electron transfer (EET) and have attracted considerable attention for their crucial role as biocatalysts of bioelectrochemical systems (BES’s). Recent studies disclosed that anode potentials affect power output and anodic microbial communities, including selection of dominant Geobacter species, in various BES’s. However, the details in current-generating properties and responses to anode potentials have been investigated only for a model species, namely Geobacter sulfurreducens. In this study, the effects of anode potentials on the current generation and the EET paths were investigated by cultivating six Geobacter species with different anode potentials, followed by electrochemical analyses. The electrochemical cultivation demonstrated that the G. metallireducens clade species (G. sulfurreducens and G. metallireducens) constantly generate high current densities at a wide range of anode potentials (≥−0.3 or −0.2 V vs. Ag/AgCl), while the subsurface clades species (G. daltonii, G. bemidjensis, G. chapellei, and G. pelophilus) generate a relatively large current only at limited potential regions (−0.1 to −0.3 V vs. Ag/AgCl). The linear sweep voltammetry analyses indicated that the G. metallireducens clade species utilize only one EET path irrespective of the anode potentials, while the subsurface clades species utilize multiple EET paths, which can be optimized depending on the anode potentials. These results clearly demonstrate that the response features to anode potentials are divergent among species (or clades) of Geobacter.
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spelling pubmed-52977422017-02-10 Influence of Anode Potentials on Current Generation and Extracellular Electron Transfer Paths of Geobacter Species Kato, Souichiro Int J Mol Sci Article Geobacter species are capable of utilizing solid-state compounds, including anodic electrodes, as electron acceptors of respiration via extracellular electron transfer (EET) and have attracted considerable attention for their crucial role as biocatalysts of bioelectrochemical systems (BES’s). Recent studies disclosed that anode potentials affect power output and anodic microbial communities, including selection of dominant Geobacter species, in various BES’s. However, the details in current-generating properties and responses to anode potentials have been investigated only for a model species, namely Geobacter sulfurreducens. In this study, the effects of anode potentials on the current generation and the EET paths were investigated by cultivating six Geobacter species with different anode potentials, followed by electrochemical analyses. The electrochemical cultivation demonstrated that the G. metallireducens clade species (G. sulfurreducens and G. metallireducens) constantly generate high current densities at a wide range of anode potentials (≥−0.3 or −0.2 V vs. Ag/AgCl), while the subsurface clades species (G. daltonii, G. bemidjensis, G. chapellei, and G. pelophilus) generate a relatively large current only at limited potential regions (−0.1 to −0.3 V vs. Ag/AgCl). The linear sweep voltammetry analyses indicated that the G. metallireducens clade species utilize only one EET path irrespective of the anode potentials, while the subsurface clades species utilize multiple EET paths, which can be optimized depending on the anode potentials. These results clearly demonstrate that the response features to anode potentials are divergent among species (or clades) of Geobacter. MDPI 2017-01-06 /pmc/articles/PMC5297742/ /pubmed/28067820 http://dx.doi.org/10.3390/ijms18010108 Text en © 2017 by the author; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kato, Souichiro
Influence of Anode Potentials on Current Generation and Extracellular Electron Transfer Paths of Geobacter Species
title Influence of Anode Potentials on Current Generation and Extracellular Electron Transfer Paths of Geobacter Species
title_full Influence of Anode Potentials on Current Generation and Extracellular Electron Transfer Paths of Geobacter Species
title_fullStr Influence of Anode Potentials on Current Generation and Extracellular Electron Transfer Paths of Geobacter Species
title_full_unstemmed Influence of Anode Potentials on Current Generation and Extracellular Electron Transfer Paths of Geobacter Species
title_short Influence of Anode Potentials on Current Generation and Extracellular Electron Transfer Paths of Geobacter Species
title_sort influence of anode potentials on current generation and extracellular electron transfer paths of geobacter species
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5297742/
https://www.ncbi.nlm.nih.gov/pubmed/28067820
http://dx.doi.org/10.3390/ijms18010108
work_keys_str_mv AT katosouichiro influenceofanodepotentialsoncurrentgenerationandextracellularelectrontransferpathsofgeobacterspecies