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The Cause of Death of a Child in the 18th Century Solved by Bone Microbiome Typing Using Laser Microdissection and Next Generation Sequencing

The history of medicine abounds in cases of mysterious deaths, especially by infectious diseases, which were probably unresolved because of the lack of knowledge and of appropriate technology. The aim of this study was to exploit contemporary technologies to try to identify the cause of death of a y...

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Autores principales: D’Argenio, Valeria, Torino, Marielva, Precone, Vincenza, Casaburi, Giorgio, Esposito, Maria Valeria, Iaffaldano, Laura, Malapelle, Umberto, Troncone, Giancarlo, Coto, Iolanda, Cavalcanti, Paolina, De Rosa, Gaetano, Salvatore, Francesco, Sacchetti, Lucia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5297743/
https://www.ncbi.nlm.nih.gov/pubmed/28067829
http://dx.doi.org/10.3390/ijms18010109
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author D’Argenio, Valeria
Torino, Marielva
Precone, Vincenza
Casaburi, Giorgio
Esposito, Maria Valeria
Iaffaldano, Laura
Malapelle, Umberto
Troncone, Giancarlo
Coto, Iolanda
Cavalcanti, Paolina
De Rosa, Gaetano
Salvatore, Francesco
Sacchetti, Lucia
author_facet D’Argenio, Valeria
Torino, Marielva
Precone, Vincenza
Casaburi, Giorgio
Esposito, Maria Valeria
Iaffaldano, Laura
Malapelle, Umberto
Troncone, Giancarlo
Coto, Iolanda
Cavalcanti, Paolina
De Rosa, Gaetano
Salvatore, Francesco
Sacchetti, Lucia
author_sort D’Argenio, Valeria
collection PubMed
description The history of medicine abounds in cases of mysterious deaths, especially by infectious diseases, which were probably unresolved because of the lack of knowledge and of appropriate technology. The aim of this study was to exploit contemporary technologies to try to identify the cause of death of a young boy who died from a putative “infection” at the end of the 18th century, and for whom an extraordinarily well-preserved minute bone fragment was available. After confirming the nature of the sample, we used laser microdissection to select the most “informative” area to be examined. Tissue genotyping indicated male gender, thereby confirming the notary’s report. 16S ribosomal RNA sequencing showed that Proteobacteria and Actinobacteria were more abundant than Firmicutes and Bacteroidetes, and that Pseudomonas was the most abundant bacterial genus in the Pseudomonadaceae family. These data suggest that the patient most likely died from Pseudomonas osteomyelitis. This case is an example of how new technological approaches, like laser microdissection and next-generation sequencing, can resolve ancient cases of uncertain etiopathology. Lastly, medical samples may contain a wealth of information that may not be accessible until more sophisticated technology becomes available. Therefore, one may envisage the possibility of systematically storing medical samples for evaluation by future generations.
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spelling pubmed-52977432017-02-10 The Cause of Death of a Child in the 18th Century Solved by Bone Microbiome Typing Using Laser Microdissection and Next Generation Sequencing D’Argenio, Valeria Torino, Marielva Precone, Vincenza Casaburi, Giorgio Esposito, Maria Valeria Iaffaldano, Laura Malapelle, Umberto Troncone, Giancarlo Coto, Iolanda Cavalcanti, Paolina De Rosa, Gaetano Salvatore, Francesco Sacchetti, Lucia Int J Mol Sci Article The history of medicine abounds in cases of mysterious deaths, especially by infectious diseases, which were probably unresolved because of the lack of knowledge and of appropriate technology. The aim of this study was to exploit contemporary technologies to try to identify the cause of death of a young boy who died from a putative “infection” at the end of the 18th century, and for whom an extraordinarily well-preserved minute bone fragment was available. After confirming the nature of the sample, we used laser microdissection to select the most “informative” area to be examined. Tissue genotyping indicated male gender, thereby confirming the notary’s report. 16S ribosomal RNA sequencing showed that Proteobacteria and Actinobacteria were more abundant than Firmicutes and Bacteroidetes, and that Pseudomonas was the most abundant bacterial genus in the Pseudomonadaceae family. These data suggest that the patient most likely died from Pseudomonas osteomyelitis. This case is an example of how new technological approaches, like laser microdissection and next-generation sequencing, can resolve ancient cases of uncertain etiopathology. Lastly, medical samples may contain a wealth of information that may not be accessible until more sophisticated technology becomes available. Therefore, one may envisage the possibility of systematically storing medical samples for evaluation by future generations. MDPI 2017-01-06 /pmc/articles/PMC5297743/ /pubmed/28067829 http://dx.doi.org/10.3390/ijms18010109 Text en © 2017 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
D’Argenio, Valeria
Torino, Marielva
Precone, Vincenza
Casaburi, Giorgio
Esposito, Maria Valeria
Iaffaldano, Laura
Malapelle, Umberto
Troncone, Giancarlo
Coto, Iolanda
Cavalcanti, Paolina
De Rosa, Gaetano
Salvatore, Francesco
Sacchetti, Lucia
The Cause of Death of a Child in the 18th Century Solved by Bone Microbiome Typing Using Laser Microdissection and Next Generation Sequencing
title The Cause of Death of a Child in the 18th Century Solved by Bone Microbiome Typing Using Laser Microdissection and Next Generation Sequencing
title_full The Cause of Death of a Child in the 18th Century Solved by Bone Microbiome Typing Using Laser Microdissection and Next Generation Sequencing
title_fullStr The Cause of Death of a Child in the 18th Century Solved by Bone Microbiome Typing Using Laser Microdissection and Next Generation Sequencing
title_full_unstemmed The Cause of Death of a Child in the 18th Century Solved by Bone Microbiome Typing Using Laser Microdissection and Next Generation Sequencing
title_short The Cause of Death of a Child in the 18th Century Solved by Bone Microbiome Typing Using Laser Microdissection and Next Generation Sequencing
title_sort cause of death of a child in the 18th century solved by bone microbiome typing using laser microdissection and next generation sequencing
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5297743/
https://www.ncbi.nlm.nih.gov/pubmed/28067829
http://dx.doi.org/10.3390/ijms18010109
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