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Affinin (Spilanthol), Isolated from Heliopsis longipes, Induces Vasodilation via Activation of Gasotransmitters and Prostacyclin Signaling Pathways

Heliopsis longipes roots have been widely used in Mexican traditional medicine to relieve pain, mainly, toothaches. Previous studies have shown that affinin, the major alkamide of these roots, induces potent antinociceptive and anti-inflammatory activities. However, the effect of H. longipes root ex...

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Autores principales: Castro-Ruiz, Jesús Eduardo, Rojas-Molina, Alejandra, Luna-Vázquez, Francisco J., Rivero-Cruz, Fausto, García-Gasca, Teresa, Ibarra-Alvarado, César
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5297847/
https://www.ncbi.nlm.nih.gov/pubmed/28117739
http://dx.doi.org/10.3390/ijms18010218
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author Castro-Ruiz, Jesús Eduardo
Rojas-Molina, Alejandra
Luna-Vázquez, Francisco J.
Rivero-Cruz, Fausto
García-Gasca, Teresa
Ibarra-Alvarado, César
author_facet Castro-Ruiz, Jesús Eduardo
Rojas-Molina, Alejandra
Luna-Vázquez, Francisco J.
Rivero-Cruz, Fausto
García-Gasca, Teresa
Ibarra-Alvarado, César
author_sort Castro-Ruiz, Jesús Eduardo
collection PubMed
description Heliopsis longipes roots have been widely used in Mexican traditional medicine to relieve pain, mainly, toothaches. Previous studies have shown that affinin, the major alkamide of these roots, induces potent antinociceptive and anti-inflammatory activities. However, the effect of H. longipes root extracts and affinin on the cardiovascular system have not been investigated so far. In the present study, we demonstrated that the dichloromethane and ethanolic extracts of H. longipes roots, and affinin, isolated from these roots, produce a concentration-dependent vasodilation of rat aorta. Affinin-induced vasorelaxation was partly dependent on the presence of endothelium and was significantly blocked in the presence of inhibitors of NO, H(2)S, and CO synthesis (N(G)-nitro-l-arginine methyl ester (l-NAME), dl-propargylglycine (PAG), and chromium mesoporphyrin (CrMP), respectively); K(+) channel blockers (glibenclamide (Gli) and tetraethyl ammonium (TEA)), and guanylate cyclase and cyclooxygenase inhibitors (1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ) and indomethacin (INDO), respectively). Our results demonstrate, for the first time, that affinin induces vasodilation by mechanisms that involve gasotransmitters, and prostacyclin signaling pathways. These findings indicate that this natural alkamide has therapeutic potential in the treatment of cardiovascular diseases.
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spelling pubmed-52978472017-02-10 Affinin (Spilanthol), Isolated from Heliopsis longipes, Induces Vasodilation via Activation of Gasotransmitters and Prostacyclin Signaling Pathways Castro-Ruiz, Jesús Eduardo Rojas-Molina, Alejandra Luna-Vázquez, Francisco J. Rivero-Cruz, Fausto García-Gasca, Teresa Ibarra-Alvarado, César Int J Mol Sci Article Heliopsis longipes roots have been widely used in Mexican traditional medicine to relieve pain, mainly, toothaches. Previous studies have shown that affinin, the major alkamide of these roots, induces potent antinociceptive and anti-inflammatory activities. However, the effect of H. longipes root extracts and affinin on the cardiovascular system have not been investigated so far. In the present study, we demonstrated that the dichloromethane and ethanolic extracts of H. longipes roots, and affinin, isolated from these roots, produce a concentration-dependent vasodilation of rat aorta. Affinin-induced vasorelaxation was partly dependent on the presence of endothelium and was significantly blocked in the presence of inhibitors of NO, H(2)S, and CO synthesis (N(G)-nitro-l-arginine methyl ester (l-NAME), dl-propargylglycine (PAG), and chromium mesoporphyrin (CrMP), respectively); K(+) channel blockers (glibenclamide (Gli) and tetraethyl ammonium (TEA)), and guanylate cyclase and cyclooxygenase inhibitors (1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ) and indomethacin (INDO), respectively). Our results demonstrate, for the first time, that affinin induces vasodilation by mechanisms that involve gasotransmitters, and prostacyclin signaling pathways. These findings indicate that this natural alkamide has therapeutic potential in the treatment of cardiovascular diseases. MDPI 2017-01-22 /pmc/articles/PMC5297847/ /pubmed/28117739 http://dx.doi.org/10.3390/ijms18010218 Text en © 2017 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Castro-Ruiz, Jesús Eduardo
Rojas-Molina, Alejandra
Luna-Vázquez, Francisco J.
Rivero-Cruz, Fausto
García-Gasca, Teresa
Ibarra-Alvarado, César
Affinin (Spilanthol), Isolated from Heliopsis longipes, Induces Vasodilation via Activation of Gasotransmitters and Prostacyclin Signaling Pathways
title Affinin (Spilanthol), Isolated from Heliopsis longipes, Induces Vasodilation via Activation of Gasotransmitters and Prostacyclin Signaling Pathways
title_full Affinin (Spilanthol), Isolated from Heliopsis longipes, Induces Vasodilation via Activation of Gasotransmitters and Prostacyclin Signaling Pathways
title_fullStr Affinin (Spilanthol), Isolated from Heliopsis longipes, Induces Vasodilation via Activation of Gasotransmitters and Prostacyclin Signaling Pathways
title_full_unstemmed Affinin (Spilanthol), Isolated from Heliopsis longipes, Induces Vasodilation via Activation of Gasotransmitters and Prostacyclin Signaling Pathways
title_short Affinin (Spilanthol), Isolated from Heliopsis longipes, Induces Vasodilation via Activation of Gasotransmitters and Prostacyclin Signaling Pathways
title_sort affinin (spilanthol), isolated from heliopsis longipes, induces vasodilation via activation of gasotransmitters and prostacyclin signaling pathways
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5297847/
https://www.ncbi.nlm.nih.gov/pubmed/28117739
http://dx.doi.org/10.3390/ijms18010218
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