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GLUT1‐mediated glucose uptake plays a crucial role during Plasmodium hepatic infection
Intracellular pathogens have evolved mechanisms to ensure their survival and development inside their host cells. Here, we show that glucose is a pivotal modulator of hepatic infection by the rodent malaria parasite Plasmodium berghei and that glucose uptake via the GLUT1 transporter is specifically...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5297879/ https://www.ncbi.nlm.nih.gov/pubmed/27404888 http://dx.doi.org/10.1111/cmi.12646 |
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author | Meireles, Patrícia Sales‐Dias, Joana Andrade, Carolina M. Mello‐Vieira, João Mancio‐Silva, Liliana Simas, J. Pedro Staines, Henry M. Prudêncio, Miguel |
author_facet | Meireles, Patrícia Sales‐Dias, Joana Andrade, Carolina M. Mello‐Vieira, João Mancio‐Silva, Liliana Simas, J. Pedro Staines, Henry M. Prudêncio, Miguel |
author_sort | Meireles, Patrícia |
collection | PubMed |
description | Intracellular pathogens have evolved mechanisms to ensure their survival and development inside their host cells. Here, we show that glucose is a pivotal modulator of hepatic infection by the rodent malaria parasite Plasmodium berghei and that glucose uptake via the GLUT1 transporter is specifically enhanced in P. berghei‐infected cells. We further show that ATP levels of cells containing developing parasites are decreased, which is known to enhance membrane GLUT1 activity. In addition, GLUT1 molecules are translocated to the membrane of the hepatic cell, increasing glucose uptake at later stages of infection. Chemical inhibition of GLUT1 activity leads to a decrease in glucose uptake and the consequent impairment of hepatic infection, both in vitro and in vivo. Our results reveal that changes in GLUT1 conformation and cellular localization seem to be part of an adaptive host response to maintain adequate cellular nutrition and energy levels, ensuring host cell survival and supporting P. berghei hepatic development. |
format | Online Article Text |
id | pubmed-5297879 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-52978792017-02-22 GLUT1‐mediated glucose uptake plays a crucial role during Plasmodium hepatic infection Meireles, Patrícia Sales‐Dias, Joana Andrade, Carolina M. Mello‐Vieira, João Mancio‐Silva, Liliana Simas, J. Pedro Staines, Henry M. Prudêncio, Miguel Cell Microbiol Original Articles Intracellular pathogens have evolved mechanisms to ensure their survival and development inside their host cells. Here, we show that glucose is a pivotal modulator of hepatic infection by the rodent malaria parasite Plasmodium berghei and that glucose uptake via the GLUT1 transporter is specifically enhanced in P. berghei‐infected cells. We further show that ATP levels of cells containing developing parasites are decreased, which is known to enhance membrane GLUT1 activity. In addition, GLUT1 molecules are translocated to the membrane of the hepatic cell, increasing glucose uptake at later stages of infection. Chemical inhibition of GLUT1 activity leads to a decrease in glucose uptake and the consequent impairment of hepatic infection, both in vitro and in vivo. Our results reveal that changes in GLUT1 conformation and cellular localization seem to be part of an adaptive host response to maintain adequate cellular nutrition and energy levels, ensuring host cell survival and supporting P. berghei hepatic development. John Wiley and Sons Inc. 2016-08-02 2017-02 /pmc/articles/PMC5297879/ /pubmed/27404888 http://dx.doi.org/10.1111/cmi.12646 Text en © 2016 The Authors Cellular Microbiology Published by John Wiley & Sons Ltd This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs (http://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Original Articles Meireles, Patrícia Sales‐Dias, Joana Andrade, Carolina M. Mello‐Vieira, João Mancio‐Silva, Liliana Simas, J. Pedro Staines, Henry M. Prudêncio, Miguel GLUT1‐mediated glucose uptake plays a crucial role during Plasmodium hepatic infection |
title | GLUT1‐mediated glucose uptake plays a crucial role during Plasmodium hepatic infection |
title_full | GLUT1‐mediated glucose uptake plays a crucial role during Plasmodium hepatic infection |
title_fullStr | GLUT1‐mediated glucose uptake plays a crucial role during Plasmodium hepatic infection |
title_full_unstemmed | GLUT1‐mediated glucose uptake plays a crucial role during Plasmodium hepatic infection |
title_short | GLUT1‐mediated glucose uptake plays a crucial role during Plasmodium hepatic infection |
title_sort | glut1‐mediated glucose uptake plays a crucial role during plasmodium hepatic infection |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5297879/ https://www.ncbi.nlm.nih.gov/pubmed/27404888 http://dx.doi.org/10.1111/cmi.12646 |
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