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Enzyme engineering: A synthetic biology approach for more effective library generation and automated high-throughput screening

The Golden Gate strategy entails the use of type IIS restriction enzymes, which cut outside of their recognition sequence. It enables unrestricted design of unique DNA fragments that can be readily and seamlessly recombined. Successfully employed in other synthetic biology applications, we demonstra...

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Detalles Bibliográficos
Autores principales:	Quaglia, Daniela, Ebert, Maximilian C. C. J. C., Mugford, Paul F., Pelletier, Joelle N.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2017
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5298319/ https://www.ncbi.nlm.nih.gov/pubmed/28178357 http://dx.doi.org/10.1371/journal.pone.0171741

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