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Normal flora of conjunctiva and lid margin, as well as its antibiotic sensitivity, in patients undergoing cataract surgery at Phramongkutklao Hospital

OBJECTIVE: This study aimed to evaluate the normal flora of conjunctiva and lid margin, as well as its antibiotic sensitivity. DESIGN: This was a prospective cross-sectional study. PATIENTS AND METHODS: A prospective study was conducted on 120 patients who underwent cataract surgery at the Phramongk...

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Autores principales: Ratnumnoi, Ravee, Keorochana, Narumon, Sontisombat, Chavalit
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5298815/
https://www.ncbi.nlm.nih.gov/pubmed/28203050
http://dx.doi.org/10.2147/OPTH.S109247
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author Ratnumnoi, Ravee
Keorochana, Narumon
Sontisombat, Chavalit
author_facet Ratnumnoi, Ravee
Keorochana, Narumon
Sontisombat, Chavalit
author_sort Ratnumnoi, Ravee
collection PubMed
description OBJECTIVE: This study aimed to evaluate the normal flora of conjunctiva and lid margin, as well as its antibiotic sensitivity. DESIGN: This was a prospective cross-sectional study. PATIENTS AND METHODS: A prospective study was conducted on 120 patients who underwent cataract surgery at the Phramongkutklao Hospital from September 2014 to October 2014. Conjunctival and lid margin swabs were obtained from patients before they underwent cataract surgery. These swabs were used to inoculate blood agar and chocolate agar plates for culturing. After growth of the normal flora, the antibiotic sensitivity method using tobramycin, moxifloxacin, levofloxacin, and cefazolin was applied. MAIN OUTCOME MEASURES: Normal flora of conjunctiva and lid margin, along with its antibiotic sensitivity, from patients who underwent cataract surgery was assessed. RESULTS: A total of 120 eyes were included in this study, and bacterial isolation rates were identified. Five bacteria from the lid margin were cultured, namely, coagulase-negative staphylococcus (58.33%), Streptococcus spp. (2.5%), Corynebacterium (1.67%), Micrococcus spp. (1.67%), and Staphylococcus aureus (0.83%). Two bacteria from the conjunctiva were cultured, namely, coagulase-negative staphylococcus (30%) and Streptococcus spp. (0.83%). Results of antibiotic sensitivity test showed that all isolated bacteria are sensitive to cefazolin 100%, tobramycin 98.67%, levofloxacin 100%, and moxifloxacin 100%. CONCLUSION: Coagulase-negative staphylococci are the most common bacteria isolated from conjunctiva and lid margin.
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spelling pubmed-52988152017-02-15 Normal flora of conjunctiva and lid margin, as well as its antibiotic sensitivity, in patients undergoing cataract surgery at Phramongkutklao Hospital Ratnumnoi, Ravee Keorochana, Narumon Sontisombat, Chavalit Clin Ophthalmol Original Research OBJECTIVE: This study aimed to evaluate the normal flora of conjunctiva and lid margin, as well as its antibiotic sensitivity. DESIGN: This was a prospective cross-sectional study. PATIENTS AND METHODS: A prospective study was conducted on 120 patients who underwent cataract surgery at the Phramongkutklao Hospital from September 2014 to October 2014. Conjunctival and lid margin swabs were obtained from patients before they underwent cataract surgery. These swabs were used to inoculate blood agar and chocolate agar plates for culturing. After growth of the normal flora, the antibiotic sensitivity method using tobramycin, moxifloxacin, levofloxacin, and cefazolin was applied. MAIN OUTCOME MEASURES: Normal flora of conjunctiva and lid margin, along with its antibiotic sensitivity, from patients who underwent cataract surgery was assessed. RESULTS: A total of 120 eyes were included in this study, and bacterial isolation rates were identified. Five bacteria from the lid margin were cultured, namely, coagulase-negative staphylococcus (58.33%), Streptococcus spp. (2.5%), Corynebacterium (1.67%), Micrococcus spp. (1.67%), and Staphylococcus aureus (0.83%). Two bacteria from the conjunctiva were cultured, namely, coagulase-negative staphylococcus (30%) and Streptococcus spp. (0.83%). Results of antibiotic sensitivity test showed that all isolated bacteria are sensitive to cefazolin 100%, tobramycin 98.67%, levofloxacin 100%, and moxifloxacin 100%. CONCLUSION: Coagulase-negative staphylococci are the most common bacteria isolated from conjunctiva and lid margin. Dove Medical Press 2017-01-27 /pmc/articles/PMC5298815/ /pubmed/28203050 http://dx.doi.org/10.2147/OPTH.S109247 Text en © 2017 Ratnumnoi et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Ratnumnoi, Ravee
Keorochana, Narumon
Sontisombat, Chavalit
Normal flora of conjunctiva and lid margin, as well as its antibiotic sensitivity, in patients undergoing cataract surgery at Phramongkutklao Hospital
title Normal flora of conjunctiva and lid margin, as well as its antibiotic sensitivity, in patients undergoing cataract surgery at Phramongkutklao Hospital
title_full Normal flora of conjunctiva and lid margin, as well as its antibiotic sensitivity, in patients undergoing cataract surgery at Phramongkutklao Hospital
title_fullStr Normal flora of conjunctiva and lid margin, as well as its antibiotic sensitivity, in patients undergoing cataract surgery at Phramongkutklao Hospital
title_full_unstemmed Normal flora of conjunctiva and lid margin, as well as its antibiotic sensitivity, in patients undergoing cataract surgery at Phramongkutklao Hospital
title_short Normal flora of conjunctiva and lid margin, as well as its antibiotic sensitivity, in patients undergoing cataract surgery at Phramongkutklao Hospital
title_sort normal flora of conjunctiva and lid margin, as well as its antibiotic sensitivity, in patients undergoing cataract surgery at phramongkutklao hospital
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5298815/
https://www.ncbi.nlm.nih.gov/pubmed/28203050
http://dx.doi.org/10.2147/OPTH.S109247
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