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Normal flora of conjunctiva and lid margin, as well as its antibiotic sensitivity, in patients undergoing cataract surgery at Phramongkutklao Hospital
OBJECTIVE: This study aimed to evaluate the normal flora of conjunctiva and lid margin, as well as its antibiotic sensitivity. DESIGN: This was a prospective cross-sectional study. PATIENTS AND METHODS: A prospective study was conducted on 120 patients who underwent cataract surgery at the Phramongk...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove Medical Press
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5298815/ https://www.ncbi.nlm.nih.gov/pubmed/28203050 http://dx.doi.org/10.2147/OPTH.S109247 |
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author | Ratnumnoi, Ravee Keorochana, Narumon Sontisombat, Chavalit |
author_facet | Ratnumnoi, Ravee Keorochana, Narumon Sontisombat, Chavalit |
author_sort | Ratnumnoi, Ravee |
collection | PubMed |
description | OBJECTIVE: This study aimed to evaluate the normal flora of conjunctiva and lid margin, as well as its antibiotic sensitivity. DESIGN: This was a prospective cross-sectional study. PATIENTS AND METHODS: A prospective study was conducted on 120 patients who underwent cataract surgery at the Phramongkutklao Hospital from September 2014 to October 2014. Conjunctival and lid margin swabs were obtained from patients before they underwent cataract surgery. These swabs were used to inoculate blood agar and chocolate agar plates for culturing. After growth of the normal flora, the antibiotic sensitivity method using tobramycin, moxifloxacin, levofloxacin, and cefazolin was applied. MAIN OUTCOME MEASURES: Normal flora of conjunctiva and lid margin, along with its antibiotic sensitivity, from patients who underwent cataract surgery was assessed. RESULTS: A total of 120 eyes were included in this study, and bacterial isolation rates were identified. Five bacteria from the lid margin were cultured, namely, coagulase-negative staphylococcus (58.33%), Streptococcus spp. (2.5%), Corynebacterium (1.67%), Micrococcus spp. (1.67%), and Staphylococcus aureus (0.83%). Two bacteria from the conjunctiva were cultured, namely, coagulase-negative staphylococcus (30%) and Streptococcus spp. (0.83%). Results of antibiotic sensitivity test showed that all isolated bacteria are sensitive to cefazolin 100%, tobramycin 98.67%, levofloxacin 100%, and moxifloxacin 100%. CONCLUSION: Coagulase-negative staphylococci are the most common bacteria isolated from conjunctiva and lid margin. |
format | Online Article Text |
id | pubmed-5298815 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-52988152017-02-15 Normal flora of conjunctiva and lid margin, as well as its antibiotic sensitivity, in patients undergoing cataract surgery at Phramongkutklao Hospital Ratnumnoi, Ravee Keorochana, Narumon Sontisombat, Chavalit Clin Ophthalmol Original Research OBJECTIVE: This study aimed to evaluate the normal flora of conjunctiva and lid margin, as well as its antibiotic sensitivity. DESIGN: This was a prospective cross-sectional study. PATIENTS AND METHODS: A prospective study was conducted on 120 patients who underwent cataract surgery at the Phramongkutklao Hospital from September 2014 to October 2014. Conjunctival and lid margin swabs were obtained from patients before they underwent cataract surgery. These swabs were used to inoculate blood agar and chocolate agar plates for culturing. After growth of the normal flora, the antibiotic sensitivity method using tobramycin, moxifloxacin, levofloxacin, and cefazolin was applied. MAIN OUTCOME MEASURES: Normal flora of conjunctiva and lid margin, along with its antibiotic sensitivity, from patients who underwent cataract surgery was assessed. RESULTS: A total of 120 eyes were included in this study, and bacterial isolation rates were identified. Five bacteria from the lid margin were cultured, namely, coagulase-negative staphylococcus (58.33%), Streptococcus spp. (2.5%), Corynebacterium (1.67%), Micrococcus spp. (1.67%), and Staphylococcus aureus (0.83%). Two bacteria from the conjunctiva were cultured, namely, coagulase-negative staphylococcus (30%) and Streptococcus spp. (0.83%). Results of antibiotic sensitivity test showed that all isolated bacteria are sensitive to cefazolin 100%, tobramycin 98.67%, levofloxacin 100%, and moxifloxacin 100%. CONCLUSION: Coagulase-negative staphylococci are the most common bacteria isolated from conjunctiva and lid margin. Dove Medical Press 2017-01-27 /pmc/articles/PMC5298815/ /pubmed/28203050 http://dx.doi.org/10.2147/OPTH.S109247 Text en © 2017 Ratnumnoi et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. |
spellingShingle | Original Research Ratnumnoi, Ravee Keorochana, Narumon Sontisombat, Chavalit Normal flora of conjunctiva and lid margin, as well as its antibiotic sensitivity, in patients undergoing cataract surgery at Phramongkutklao Hospital |
title | Normal flora of conjunctiva and lid margin, as well as its antibiotic sensitivity, in patients undergoing cataract surgery at Phramongkutklao Hospital |
title_full | Normal flora of conjunctiva and lid margin, as well as its antibiotic sensitivity, in patients undergoing cataract surgery at Phramongkutklao Hospital |
title_fullStr | Normal flora of conjunctiva and lid margin, as well as its antibiotic sensitivity, in patients undergoing cataract surgery at Phramongkutklao Hospital |
title_full_unstemmed | Normal flora of conjunctiva and lid margin, as well as its antibiotic sensitivity, in patients undergoing cataract surgery at Phramongkutklao Hospital |
title_short | Normal flora of conjunctiva and lid margin, as well as its antibiotic sensitivity, in patients undergoing cataract surgery at Phramongkutklao Hospital |
title_sort | normal flora of conjunctiva and lid margin, as well as its antibiotic sensitivity, in patients undergoing cataract surgery at phramongkutklao hospital |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5298815/ https://www.ncbi.nlm.nih.gov/pubmed/28203050 http://dx.doi.org/10.2147/OPTH.S109247 |
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