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Correlation between Infective Factors and Antibiotic Resistance in Enterococci Clinical Isolates in West of Iran

The present study was done to scrutinize the possible relation between infective genes and antimicrobial resistance in Enterococcus faecalis and Enterococcus faecium. Considering the fact that the presence of recognized infective determinants among clinical isolates may promote the emergence of infe...

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Autores principales: Arabestani, Mohammad Reza, Nasaj, Mona, Mousavi, Seyed Masoud
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Chonnam National University Medical School 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5299130/
https://www.ncbi.nlm.nih.gov/pubmed/28184339
http://dx.doi.org/10.4068/cmj.2017.53.1.56
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author Arabestani, Mohammad Reza
Nasaj, Mona
Mousavi, Seyed Masoud
author_facet Arabestani, Mohammad Reza
Nasaj, Mona
Mousavi, Seyed Masoud
author_sort Arabestani, Mohammad Reza
collection PubMed
description The present study was done to scrutinize the possible relation between infective genes and antimicrobial resistance in Enterococcus faecalis and Enterococcus faecium. Considering the fact that the presence of recognized infective determinants among clinical isolates may promote the emergence of infections and persistence of Enterococci in hospital settings, which can lead to an increase in antimicrobial resistance. 175 E. faecalis and 67 E. faecium isolated from clinical specimens were used. The isolates were identified, and then antibiotic susceptibility testing was performed. The MIC of vancomycin and teicoplanin were determined by broth microdilution method. The presence of infective genes esp, hyl and asa(1) was scrutinized using PCR. Of the 280 enterococcal isolates, 175 (62.5%) isolates were identified as E. faecalis, 67 (24%) as E. faecium and 38 (13.5%) as Enterococcus spp. The results of the antibiotic susceptibility testing showed resistance rates of 5% and 73% to vancomycin and teicoplanin in E. faecalis and E. faecium isolates, respectively. The statistical analysis showed that the esp infective gene has significant associations with ciprofloxacin, erythromycin and tetracycline in E. faecium and with chloramphenicol in E. faecalis strains; the hyl with teicoplanin and vancomycin in E. faecium strains; and also asa(1) with vancomycin in E. faecium and with ampicillin and chloramphenicol in E. faecalis strains. Regarding the relationships between virulence genes and antibiotic resistance in strains of E. faecalis and E. faecium, detection of infective factors associated with invasive diseases has become a major issue of concern.
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spelling pubmed-52991302017-02-09 Correlation between Infective Factors and Antibiotic Resistance in Enterococci Clinical Isolates in West of Iran Arabestani, Mohammad Reza Nasaj, Mona Mousavi, Seyed Masoud Chonnam Med J Original Article The present study was done to scrutinize the possible relation between infective genes and antimicrobial resistance in Enterococcus faecalis and Enterococcus faecium. Considering the fact that the presence of recognized infective determinants among clinical isolates may promote the emergence of infections and persistence of Enterococci in hospital settings, which can lead to an increase in antimicrobial resistance. 175 E. faecalis and 67 E. faecium isolated from clinical specimens were used. The isolates were identified, and then antibiotic susceptibility testing was performed. The MIC of vancomycin and teicoplanin were determined by broth microdilution method. The presence of infective genes esp, hyl and asa(1) was scrutinized using PCR. Of the 280 enterococcal isolates, 175 (62.5%) isolates were identified as E. faecalis, 67 (24%) as E. faecium and 38 (13.5%) as Enterococcus spp. The results of the antibiotic susceptibility testing showed resistance rates of 5% and 73% to vancomycin and teicoplanin in E. faecalis and E. faecium isolates, respectively. The statistical analysis showed that the esp infective gene has significant associations with ciprofloxacin, erythromycin and tetracycline in E. faecium and with chloramphenicol in E. faecalis strains; the hyl with teicoplanin and vancomycin in E. faecium strains; and also asa(1) with vancomycin in E. faecium and with ampicillin and chloramphenicol in E. faecalis strains. Regarding the relationships between virulence genes and antibiotic resistance in strains of E. faecalis and E. faecium, detection of infective factors associated with invasive diseases has become a major issue of concern. Chonnam National University Medical School 2017-01 2017-01-25 /pmc/articles/PMC5299130/ /pubmed/28184339 http://dx.doi.org/10.4068/cmj.2017.53.1.56 Text en © Chonnam Medical Journal, 2017 http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Arabestani, Mohammad Reza
Nasaj, Mona
Mousavi, Seyed Masoud
Correlation between Infective Factors and Antibiotic Resistance in Enterococci Clinical Isolates in West of Iran
title Correlation between Infective Factors and Antibiotic Resistance in Enterococci Clinical Isolates in West of Iran
title_full Correlation between Infective Factors and Antibiotic Resistance in Enterococci Clinical Isolates in West of Iran
title_fullStr Correlation between Infective Factors and Antibiotic Resistance in Enterococci Clinical Isolates in West of Iran
title_full_unstemmed Correlation between Infective Factors and Antibiotic Resistance in Enterococci Clinical Isolates in West of Iran
title_short Correlation between Infective Factors and Antibiotic Resistance in Enterococci Clinical Isolates in West of Iran
title_sort correlation between infective factors and antibiotic resistance in enterococci clinical isolates in west of iran
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5299130/
https://www.ncbi.nlm.nih.gov/pubmed/28184339
http://dx.doi.org/10.4068/cmj.2017.53.1.56
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