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Effects of Herb-Partitioned Moxibustion on the miRNA Expression Profiles in Colon from Rats with DSS-Induced Ulcerative Colitis

Objective. This study explored the mechanism of herb-partitioned moxibustion (HM) on dextran sulfate sodium- (DSS-) induced ulcerative colitis (UC) from the miRNA perspective. Methods. Rats were randomly divided into 3 groups [normal control (NC) group, UC model (UC) group, and herb-partitioned moxi...

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Autores principales: Huang, Yan, Ma, Zhe, Cui, Yun-hua, Dong, Hong-sheng, Zhao, Ji-meng, Dou, Chuan-zi, Liu, Hui-rong, Li, Jing, Wu, Huan-gan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5299174/
https://www.ncbi.nlm.nih.gov/pubmed/28246536
http://dx.doi.org/10.1155/2017/1767301
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author Huang, Yan
Ma, Zhe
Cui, Yun-hua
Dong, Hong-sheng
Zhao, Ji-meng
Dou, Chuan-zi
Liu, Hui-rong
Li, Jing
Wu, Huan-gan
author_facet Huang, Yan
Ma, Zhe
Cui, Yun-hua
Dong, Hong-sheng
Zhao, Ji-meng
Dou, Chuan-zi
Liu, Hui-rong
Li, Jing
Wu, Huan-gan
author_sort Huang, Yan
collection PubMed
description Objective. This study explored the mechanism of herb-partitioned moxibustion (HM) on dextran sulfate sodium- (DSS-) induced ulcerative colitis (UC) from the miRNA perspective. Methods. Rats were randomly divided into 3 groups [normal control (NC) group, UC model (UC) group, and herb-partitioned moxibustion (UCHM) group]. The UC and UCHM groups were administered 4% DSS for 7 days. The UCHM group received HM at the Tianshu (bilateral, ST25). The effect of HM on UC was observed and the miRNA expression profile in the colon tissues was analyzed. Results. Compared with the UC group, the body weights were significantly higher in the UCHM group on day 14 (P < 0.001); the macroscopic colon injury scores and microscopic histopathology scores in the UCHM group decreased (P < 0.05); and there were 15 differentially expressed miRNAs in the UCHM group. The changes in miR-184 and miR-490-5p expression levels on the UC were reversed by HM intervention. Validation using qRT-PCR showed that two miRNAs expression trend was consistent with the sequencing results. Conclusion. HM at ST25 might regulate miR-184 and miR-490-5p expression, act on the transcription of their target genes to regulate inflammatory signaling pathways, and attenuate inflammation and tissue injury in the colons of rats with DSS-induced UC.
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spelling pubmed-52991742017-02-28 Effects of Herb-Partitioned Moxibustion on the miRNA Expression Profiles in Colon from Rats with DSS-Induced Ulcerative Colitis Huang, Yan Ma, Zhe Cui, Yun-hua Dong, Hong-sheng Zhao, Ji-meng Dou, Chuan-zi Liu, Hui-rong Li, Jing Wu, Huan-gan Evid Based Complement Alternat Med Research Article Objective. This study explored the mechanism of herb-partitioned moxibustion (HM) on dextran sulfate sodium- (DSS-) induced ulcerative colitis (UC) from the miRNA perspective. Methods. Rats were randomly divided into 3 groups [normal control (NC) group, UC model (UC) group, and herb-partitioned moxibustion (UCHM) group]. The UC and UCHM groups were administered 4% DSS for 7 days. The UCHM group received HM at the Tianshu (bilateral, ST25). The effect of HM on UC was observed and the miRNA expression profile in the colon tissues was analyzed. Results. Compared with the UC group, the body weights were significantly higher in the UCHM group on day 14 (P < 0.001); the macroscopic colon injury scores and microscopic histopathology scores in the UCHM group decreased (P < 0.05); and there were 15 differentially expressed miRNAs in the UCHM group. The changes in miR-184 and miR-490-5p expression levels on the UC were reversed by HM intervention. Validation using qRT-PCR showed that two miRNAs expression trend was consistent with the sequencing results. Conclusion. HM at ST25 might regulate miR-184 and miR-490-5p expression, act on the transcription of their target genes to regulate inflammatory signaling pathways, and attenuate inflammation and tissue injury in the colons of rats with DSS-induced UC. Hindawi Publishing Corporation 2017 2017-01-26 /pmc/articles/PMC5299174/ /pubmed/28246536 http://dx.doi.org/10.1155/2017/1767301 Text en Copyright © 2017 Yan Huang et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Huang, Yan
Ma, Zhe
Cui, Yun-hua
Dong, Hong-sheng
Zhao, Ji-meng
Dou, Chuan-zi
Liu, Hui-rong
Li, Jing
Wu, Huan-gan
Effects of Herb-Partitioned Moxibustion on the miRNA Expression Profiles in Colon from Rats with DSS-Induced Ulcerative Colitis
title Effects of Herb-Partitioned Moxibustion on the miRNA Expression Profiles in Colon from Rats with DSS-Induced Ulcerative Colitis
title_full Effects of Herb-Partitioned Moxibustion on the miRNA Expression Profiles in Colon from Rats with DSS-Induced Ulcerative Colitis
title_fullStr Effects of Herb-Partitioned Moxibustion on the miRNA Expression Profiles in Colon from Rats with DSS-Induced Ulcerative Colitis
title_full_unstemmed Effects of Herb-Partitioned Moxibustion on the miRNA Expression Profiles in Colon from Rats with DSS-Induced Ulcerative Colitis
title_short Effects of Herb-Partitioned Moxibustion on the miRNA Expression Profiles in Colon from Rats with DSS-Induced Ulcerative Colitis
title_sort effects of herb-partitioned moxibustion on the mirna expression profiles in colon from rats with dss-induced ulcerative colitis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5299174/
https://www.ncbi.nlm.nih.gov/pubmed/28246536
http://dx.doi.org/10.1155/2017/1767301
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