An air-liquid interphase approach for modeling the early embryo-maternal contact zone

We developed an air-liquid interphase culture procedure for mammalian oviduct epithelial cells leading to the formation of functional epithelial tissues, which generate oviduct fluid surrogates. These in vitro oviduct epithelia can be co-cultured with living zygotes and enable embryonic development...

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Autores principales: Chen, S., Palma-Vera, S. E., Langhammer, M., Galuska, S. P., Braun, B. C., Krause, E., Lucas-Hahn, A., Schoen, J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5299422/
https://www.ncbi.nlm.nih.gov/pubmed/28181558
http://dx.doi.org/10.1038/srep42298
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author Chen, S.
Palma-Vera, S. E.
Langhammer, M.
Galuska, S. P.
Braun, B. C.
Krause, E.
Lucas-Hahn, A.
Schoen, J.
author_facet Chen, S.
Palma-Vera, S. E.
Langhammer, M.
Galuska, S. P.
Braun, B. C.
Krause, E.
Lucas-Hahn, A.
Schoen, J.
author_sort Chen, S.
collection PubMed
description We developed an air-liquid interphase culture procedure for mammalian oviduct epithelial cells leading to the formation of functional epithelial tissues, which generate oviduct fluid surrogates. These in vitro oviduct epithelia can be co-cultured with living zygotes and enable embryonic development up to the blastocyst stage without addition of embryo culture medium. The described strategy is broadly applicable to analyze early embryo-maternal interactions under standardized in vitro conditions.
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spelling pubmed-52994222017-02-13 An air-liquid interphase approach for modeling the early embryo-maternal contact zone Chen, S. Palma-Vera, S. E. Langhammer, M. Galuska, S. P. Braun, B. C. Krause, E. Lucas-Hahn, A. Schoen, J. Sci Rep Article We developed an air-liquid interphase culture procedure for mammalian oviduct epithelial cells leading to the formation of functional epithelial tissues, which generate oviduct fluid surrogates. These in vitro oviduct epithelia can be co-cultured with living zygotes and enable embryonic development up to the blastocyst stage without addition of embryo culture medium. The described strategy is broadly applicable to analyze early embryo-maternal interactions under standardized in vitro conditions. Nature Publishing Group 2017-02-09 /pmc/articles/PMC5299422/ /pubmed/28181558 http://dx.doi.org/10.1038/srep42298 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Chen, S.
Palma-Vera, S. E.
Langhammer, M.
Galuska, S. P.
Braun, B. C.
Krause, E.
Lucas-Hahn, A.
Schoen, J.
An air-liquid interphase approach for modeling the early embryo-maternal contact zone
title An air-liquid interphase approach for modeling the early embryo-maternal contact zone
title_full An air-liquid interphase approach for modeling the early embryo-maternal contact zone
title_fullStr An air-liquid interphase approach for modeling the early embryo-maternal contact zone
title_full_unstemmed An air-liquid interphase approach for modeling the early embryo-maternal contact zone
title_short An air-liquid interphase approach for modeling the early embryo-maternal contact zone
title_sort air-liquid interphase approach for modeling the early embryo-maternal contact zone
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5299422/
https://www.ncbi.nlm.nih.gov/pubmed/28181558
http://dx.doi.org/10.1038/srep42298
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