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Platelet responses to agonists in a cohort of highly characterised platelet donors are consistent over time

BACKGROUND AND OBJECTIVES: Platelet function shows significant inheritance that is at least partially genetically controlled. There is also evidence that the platelet response is stable over time, but there are few studies that have assessed consistency of platelet function over months and years. We...

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Autores principales: Garner, S. F., Furnell, A., Kahan, B. C., Jones, C. I., Attwood, A., Harrison, P., Kelly, A. M., Goodall, A. H., Cardigan, R., Ouwehand, W. H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5299478/
https://www.ncbi.nlm.nih.gov/pubmed/28001309
http://dx.doi.org/10.1111/vox.12468
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author Garner, S. F.
Furnell, A.
Kahan, B. C.
Jones, C. I.
Attwood, A.
Harrison, P.
Kelly, A. M.
Goodall, A. H.
Cardigan, R.
Ouwehand, W. H.
author_facet Garner, S. F.
Furnell, A.
Kahan, B. C.
Jones, C. I.
Attwood, A.
Harrison, P.
Kelly, A. M.
Goodall, A. H.
Cardigan, R.
Ouwehand, W. H.
author_sort Garner, S. F.
collection PubMed
description BACKGROUND AND OBJECTIVES: Platelet function shows significant inheritance that is at least partially genetically controlled. There is also evidence that the platelet response is stable over time, but there are few studies that have assessed consistency of platelet function over months and years. We aimed to measure platelet function in platelet donors over time in individuals selected from a cohort of 956 donors whose platelet function had been previously characterised. MATERIALS AND METHODS: Platelet function was assessed by flow cytometry, measuring fibrinogen binding and P‐selectin expression after stimulation with either cross‐linked collagen‐related peptide or adenosine 5′‐diphosphate. Eighty‐nine donors from the Cambridge Platelet Function Cohort whose platelet responses were initially within the lower or upper decile of reactivity were retested between 4 months and five and a half years later. RESULTS: There was moderate‐to‐high correlation between the initial and repeat platelet function results for all assays (P ≤ 0·007, r (2) 0·2961–0·7625); furthermore, the range of results observed in the initial low and high responder groups remained significantly different at the time of the second test (P ≤ 0·0005). CONCLUSION: Platelet function remains consistent over time. This implies that this potential influence on quality of donated platelet concentrates will remain essentially constant for a given donor.
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spelling pubmed-52994782017-02-22 Platelet responses to agonists in a cohort of highly characterised platelet donors are consistent over time Garner, S. F. Furnell, A. Kahan, B. C. Jones, C. I. Attwood, A. Harrison, P. Kelly, A. M. Goodall, A. H. Cardigan, R. Ouwehand, W. H. Vox Sang Blood Component Collection and Production BACKGROUND AND OBJECTIVES: Platelet function shows significant inheritance that is at least partially genetically controlled. There is also evidence that the platelet response is stable over time, but there are few studies that have assessed consistency of platelet function over months and years. We aimed to measure platelet function in platelet donors over time in individuals selected from a cohort of 956 donors whose platelet function had been previously characterised. MATERIALS AND METHODS: Platelet function was assessed by flow cytometry, measuring fibrinogen binding and P‐selectin expression after stimulation with either cross‐linked collagen‐related peptide or adenosine 5′‐diphosphate. Eighty‐nine donors from the Cambridge Platelet Function Cohort whose platelet responses were initially within the lower or upper decile of reactivity were retested between 4 months and five and a half years later. RESULTS: There was moderate‐to‐high correlation between the initial and repeat platelet function results for all assays (P ≤ 0·007, r (2) 0·2961–0·7625); furthermore, the range of results observed in the initial low and high responder groups remained significantly different at the time of the second test (P ≤ 0·0005). CONCLUSION: Platelet function remains consistent over time. This implies that this potential influence on quality of donated platelet concentrates will remain essentially constant for a given donor. John Wiley and Sons Inc. 2016-12-21 2017-01 /pmc/articles/PMC5299478/ /pubmed/28001309 http://dx.doi.org/10.1111/vox.12468 Text en © 2016 The Authors. Vox Sanguinis published by John Wiley & Sons Ltd on behalf of International Society of Blood Transfusion. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Blood Component Collection and Production
Garner, S. F.
Furnell, A.
Kahan, B. C.
Jones, C. I.
Attwood, A.
Harrison, P.
Kelly, A. M.
Goodall, A. H.
Cardigan, R.
Ouwehand, W. H.
Platelet responses to agonists in a cohort of highly characterised platelet donors are consistent over time
title Platelet responses to agonists in a cohort of highly characterised platelet donors are consistent over time
title_full Platelet responses to agonists in a cohort of highly characterised platelet donors are consistent over time
title_fullStr Platelet responses to agonists in a cohort of highly characterised platelet donors are consistent over time
title_full_unstemmed Platelet responses to agonists in a cohort of highly characterised platelet donors are consistent over time
title_short Platelet responses to agonists in a cohort of highly characterised platelet donors are consistent over time
title_sort platelet responses to agonists in a cohort of highly characterised platelet donors are consistent over time
topic Blood Component Collection and Production
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5299478/
https://www.ncbi.nlm.nih.gov/pubmed/28001309
http://dx.doi.org/10.1111/vox.12468
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