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Genome‐wide analysis of canonical Wnt target gene regulation in Xenopus tropicalis challenges β‐catenin paradigm
Wnt/β‐catenin signaling is an important cell‐to‐cell signaling mechanism that controls gene expression during embryonic development and is critically implicated in human diseases. Developmental, cellular, and transcriptional responses to Wnt signaling are remarkably context‐specific in different bio...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5299483/ https://www.ncbi.nlm.nih.gov/pubmed/28095618 http://dx.doi.org/10.1002/dvg.22991 |
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author | Nakamura, Yukio Hoppler, Stefan |
author_facet | Nakamura, Yukio Hoppler, Stefan |
author_sort | Nakamura, Yukio |
collection | PubMed |
description | Wnt/β‐catenin signaling is an important cell‐to‐cell signaling mechanism that controls gene expression during embryonic development and is critically implicated in human diseases. Developmental, cellular, and transcriptional responses to Wnt signaling are remarkably context‐specific in different biological processes. While nuclear localization of β‐catenin is the key to activation of the Wnt/β‐catenin pathway and target gene expression, the molecular mechanisms of how the same Wnt/β‐catenin signaling pathway induces specific responses remain undetermined. Recent advances in high‐throughput sequencing technologies and the availability of genome information for Xenopus tropicalis have enabled us to uncover a genome‐wide view of Wnt/β‐catenin signaling in early vertebrate embryos, which challenges previous concepts about molecular mechanisms of Wnt target gene regulation. In this review, we summarize our experimental approaches, introduce the technologies we employed and focus on recent findings about Wnt target gene regulation from Xenopus research. We will also discuss potential functions of widespread β‐catenin binding in the genome that we discovered in this species. |
format | Online Article Text |
id | pubmed-5299483 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-52994832017-02-22 Genome‐wide analysis of canonical Wnt target gene regulation in Xenopus tropicalis challenges β‐catenin paradigm Nakamura, Yukio Hoppler, Stefan Genesis Signaling and Morphogenesis Wnt/β‐catenin signaling is an important cell‐to‐cell signaling mechanism that controls gene expression during embryonic development and is critically implicated in human diseases. Developmental, cellular, and transcriptional responses to Wnt signaling are remarkably context‐specific in different biological processes. While nuclear localization of β‐catenin is the key to activation of the Wnt/β‐catenin pathway and target gene expression, the molecular mechanisms of how the same Wnt/β‐catenin signaling pathway induces specific responses remain undetermined. Recent advances in high‐throughput sequencing technologies and the availability of genome information for Xenopus tropicalis have enabled us to uncover a genome‐wide view of Wnt/β‐catenin signaling in early vertebrate embryos, which challenges previous concepts about molecular mechanisms of Wnt target gene regulation. In this review, we summarize our experimental approaches, introduce the technologies we employed and focus on recent findings about Wnt target gene regulation from Xenopus research. We will also discuss potential functions of widespread β‐catenin binding in the genome that we discovered in this species. John Wiley and Sons Inc. 2017-01-29 2017-01 /pmc/articles/PMC5299483/ /pubmed/28095618 http://dx.doi.org/10.1002/dvg.22991 Text en © 2017 The Authors genesis Published by Wiley Periodicals, Inc. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Signaling and Morphogenesis Nakamura, Yukio Hoppler, Stefan Genome‐wide analysis of canonical Wnt target gene regulation in Xenopus tropicalis challenges β‐catenin paradigm |
title | Genome‐wide analysis of canonical Wnt target gene regulation in Xenopus tropicalis challenges β‐catenin paradigm |
title_full | Genome‐wide analysis of canonical Wnt target gene regulation in Xenopus tropicalis challenges β‐catenin paradigm |
title_fullStr | Genome‐wide analysis of canonical Wnt target gene regulation in Xenopus tropicalis challenges β‐catenin paradigm |
title_full_unstemmed | Genome‐wide analysis of canonical Wnt target gene regulation in Xenopus tropicalis challenges β‐catenin paradigm |
title_short | Genome‐wide analysis of canonical Wnt target gene regulation in Xenopus tropicalis challenges β‐catenin paradigm |
title_sort | genome‐wide analysis of canonical wnt target gene regulation in xenopus tropicalis challenges β‐catenin paradigm |
topic | Signaling and Morphogenesis |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5299483/ https://www.ncbi.nlm.nih.gov/pubmed/28095618 http://dx.doi.org/10.1002/dvg.22991 |
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