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Genistein treatment improves fracture resistance in obese diabetic mice
BACKGROUND: Obese, type two diabetics are at an increased risk for fracturing their limb bones in comparison to the general population. Phytoestrogens like as the soy isoflavone genistein have been shown to protect against bone loss. In this study, we tested the effects of genistein treatment on fem...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5299772/ https://www.ncbi.nlm.nih.gov/pubmed/28183304 http://dx.doi.org/10.1186/s12902-016-0144-4 |
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author | Odle, Britton Dennison, Nathan Al-Nakkash, Layla Broderick, Tom L. Plochocki, Jeffrey H. |
author_facet | Odle, Britton Dennison, Nathan Al-Nakkash, Layla Broderick, Tom L. Plochocki, Jeffrey H. |
author_sort | Odle, Britton |
collection | PubMed |
description | BACKGROUND: Obese, type two diabetics are at an increased risk for fracturing their limb bones in comparison to the general population. Phytoestrogens like as the soy isoflavone genistein have been shown to protect against bone loss. In this study, we tested the effects of genistein treatment on femurs of ob/ob mice, a model for obesity and type two diabetes mellitus. METHODS: Twenty six-week-old female mice were divided into obese (ob/ob) control, obese genistein-treated, lean (ob/+) control, and lean genistein-treated groups (n = 5 each). Treatment with genistein consisted of 600 mg genistein/kg diet. Control mice were given standard rodent chow. At the end of a four-week treatment period, bone histomorphometric and three-point bending properties were compared among groups. RESULTS: Obese mice had larger bone areas (B.Ar.; P < 0.05) and total areas (Tt.Ar.; P < 0.05), but similar bone volume (B.Ar./Tt.Ar.; P > 0.05) of the proximal femoral epiphysis in comparison to lean mice. Treatment with genistein decreased Tt.Ar. and femur length, and increased ultimate force required to fracture the femur and the maximum deformation to failure (P < 0.05). CONCLUSIONS: Genistein improves resistance to fracture from bending loads. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12902-016-0144-4) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-5299772 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-52997722017-02-13 Genistein treatment improves fracture resistance in obese diabetic mice Odle, Britton Dennison, Nathan Al-Nakkash, Layla Broderick, Tom L. Plochocki, Jeffrey H. BMC Endocr Disord Research Article BACKGROUND: Obese, type two diabetics are at an increased risk for fracturing their limb bones in comparison to the general population. Phytoestrogens like as the soy isoflavone genistein have been shown to protect against bone loss. In this study, we tested the effects of genistein treatment on femurs of ob/ob mice, a model for obesity and type two diabetes mellitus. METHODS: Twenty six-week-old female mice were divided into obese (ob/ob) control, obese genistein-treated, lean (ob/+) control, and lean genistein-treated groups (n = 5 each). Treatment with genistein consisted of 600 mg genistein/kg diet. Control mice were given standard rodent chow. At the end of a four-week treatment period, bone histomorphometric and three-point bending properties were compared among groups. RESULTS: Obese mice had larger bone areas (B.Ar.; P < 0.05) and total areas (Tt.Ar.; P < 0.05), but similar bone volume (B.Ar./Tt.Ar.; P > 0.05) of the proximal femoral epiphysis in comparison to lean mice. Treatment with genistein decreased Tt.Ar. and femur length, and increased ultimate force required to fracture the femur and the maximum deformation to failure (P < 0.05). CONCLUSIONS: Genistein improves resistance to fracture from bending loads. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12902-016-0144-4) contains supplementary material, which is available to authorized users. BioMed Central 2017-01-09 /pmc/articles/PMC5299772/ /pubmed/28183304 http://dx.doi.org/10.1186/s12902-016-0144-4 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Odle, Britton Dennison, Nathan Al-Nakkash, Layla Broderick, Tom L. Plochocki, Jeffrey H. Genistein treatment improves fracture resistance in obese diabetic mice |
title | Genistein treatment improves fracture resistance in obese diabetic mice |
title_full | Genistein treatment improves fracture resistance in obese diabetic mice |
title_fullStr | Genistein treatment improves fracture resistance in obese diabetic mice |
title_full_unstemmed | Genistein treatment improves fracture resistance in obese diabetic mice |
title_short | Genistein treatment improves fracture resistance in obese diabetic mice |
title_sort | genistein treatment improves fracture resistance in obese diabetic mice |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5299772/ https://www.ncbi.nlm.nih.gov/pubmed/28183304 http://dx.doi.org/10.1186/s12902-016-0144-4 |
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