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Expanding the archaellum regulatory network – the eukaryotic protein kinases ArnC and ArnD influence motility of Sulfolobus acidocaldarius

Expression of the archaellum, the archaeal‐type IV pilus‐like rotating motility structure is upregulated under nutrient limitation. This is controlled by a network of regulators, called the archaellum regulatory network (arn). Several of the components of this network in Sulfolobus acidocaldarius ca...

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Autores principales: Hoffmann, Lena, Schummer, Andreas, Reimann, Julia, Haurat, Maria F., Wilson, Amanda J., Beeby, Morgan, Warscheid, Bettina, Albers, Sonja‐V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5300886/
https://www.ncbi.nlm.nih.gov/pubmed/27771939
http://dx.doi.org/10.1002/mbo3.414
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author Hoffmann, Lena
Schummer, Andreas
Reimann, Julia
Haurat, Maria F.
Wilson, Amanda J.
Beeby, Morgan
Warscheid, Bettina
Albers, Sonja‐V.
author_facet Hoffmann, Lena
Schummer, Andreas
Reimann, Julia
Haurat, Maria F.
Wilson, Amanda J.
Beeby, Morgan
Warscheid, Bettina
Albers, Sonja‐V.
author_sort Hoffmann, Lena
collection PubMed
description Expression of the archaellum, the archaeal‐type IV pilus‐like rotating motility structure is upregulated under nutrient limitation. This is controlled by a network of regulators, called the archaellum regulatory network (arn). Several of the components of this network in Sulfolobus acidocaldarius can be phosphorylated, and the deletion of the phosphatase PP2A results in strongly increased motility during starvation, indicating a role for phosphorylation in the regulation of motility. Analysis of the motility of different protein kinase deletion strains revealed that deletion of saci_0965, saci_1181, and saci_1193 resulted in reduced motility, whereas the deletion of saci_1694 resulted in hypermotility. Here ArnC (Saci_1193) and ArnD (Saci_1694) are characterized. Purified ArnC and ArnD phosphorylate serine and threonine residues in the C‐terminus of the repressor ArnB. arnC is upregulated in starvation medium, whereas arnD is constitutively expressed. However, while differences in the expression and levels of flaB were observed in the ΔarnD strain during growth under rich conditions, under nutrient limiting conditions the ΔarnC and ΔarnD strains showed no large differences in the expression levels of the archaellum or of the studied regulators. This suggests that next to the regulation via the archaellum regulatory network additional regulatory mechanisms of expression and/or activity of the archaellum exist.
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spelling pubmed-53008862017-02-13 Expanding the archaellum regulatory network – the eukaryotic protein kinases ArnC and ArnD influence motility of Sulfolobus acidocaldarius Hoffmann, Lena Schummer, Andreas Reimann, Julia Haurat, Maria F. Wilson, Amanda J. Beeby, Morgan Warscheid, Bettina Albers, Sonja‐V. Microbiologyopen Original Research Expression of the archaellum, the archaeal‐type IV pilus‐like rotating motility structure is upregulated under nutrient limitation. This is controlled by a network of regulators, called the archaellum regulatory network (arn). Several of the components of this network in Sulfolobus acidocaldarius can be phosphorylated, and the deletion of the phosphatase PP2A results in strongly increased motility during starvation, indicating a role for phosphorylation in the regulation of motility. Analysis of the motility of different protein kinase deletion strains revealed that deletion of saci_0965, saci_1181, and saci_1193 resulted in reduced motility, whereas the deletion of saci_1694 resulted in hypermotility. Here ArnC (Saci_1193) and ArnD (Saci_1694) are characterized. Purified ArnC and ArnD phosphorylate serine and threonine residues in the C‐terminus of the repressor ArnB. arnC is upregulated in starvation medium, whereas arnD is constitutively expressed. However, while differences in the expression and levels of flaB were observed in the ΔarnD strain during growth under rich conditions, under nutrient limiting conditions the ΔarnC and ΔarnD strains showed no large differences in the expression levels of the archaellum or of the studied regulators. This suggests that next to the regulation via the archaellum regulatory network additional regulatory mechanisms of expression and/or activity of the archaellum exist. John Wiley and Sons Inc. 2016-10-22 /pmc/articles/PMC5300886/ /pubmed/27771939 http://dx.doi.org/10.1002/mbo3.414 Text en © 2016 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research
Hoffmann, Lena
Schummer, Andreas
Reimann, Julia
Haurat, Maria F.
Wilson, Amanda J.
Beeby, Morgan
Warscheid, Bettina
Albers, Sonja‐V.
Expanding the archaellum regulatory network – the eukaryotic protein kinases ArnC and ArnD influence motility of Sulfolobus acidocaldarius
title Expanding the archaellum regulatory network – the eukaryotic protein kinases ArnC and ArnD influence motility of Sulfolobus acidocaldarius
title_full Expanding the archaellum regulatory network – the eukaryotic protein kinases ArnC and ArnD influence motility of Sulfolobus acidocaldarius
title_fullStr Expanding the archaellum regulatory network – the eukaryotic protein kinases ArnC and ArnD influence motility of Sulfolobus acidocaldarius
title_full_unstemmed Expanding the archaellum regulatory network – the eukaryotic protein kinases ArnC and ArnD influence motility of Sulfolobus acidocaldarius
title_short Expanding the archaellum regulatory network – the eukaryotic protein kinases ArnC and ArnD influence motility of Sulfolobus acidocaldarius
title_sort expanding the archaellum regulatory network – the eukaryotic protein kinases arnc and arnd influence motility of sulfolobus acidocaldarius
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5300886/
https://www.ncbi.nlm.nih.gov/pubmed/27771939
http://dx.doi.org/10.1002/mbo3.414
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