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Pan-retinal characterisation of Light Responses from Ganglion Cells in the Developing Mouse Retina

We have investigated the ontogeny of light-driven responses in mouse retinal ganglion cells (RGCs). Using a large-scale, high-density multielectrode array, we recorded from hundreds to thousands of RGCs simultaneously at pan-retinal level, including dorsal and ventral locations. Responses to differe...

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Autores principales: Hilgen, Gerrit, Pirmoradian, Sahar, Pamplona, Daniela, Kornprobst, Pierre, Cessac, Bruno, Hennig, Matthias H., Sernagor, Evelyne
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5301206/
https://www.ncbi.nlm.nih.gov/pubmed/28186129
http://dx.doi.org/10.1038/srep42330
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author Hilgen, Gerrit
Pirmoradian, Sahar
Pamplona, Daniela
Kornprobst, Pierre
Cessac, Bruno
Hennig, Matthias H.
Sernagor, Evelyne
author_facet Hilgen, Gerrit
Pirmoradian, Sahar
Pamplona, Daniela
Kornprobst, Pierre
Cessac, Bruno
Hennig, Matthias H.
Sernagor, Evelyne
author_sort Hilgen, Gerrit
collection PubMed
description We have investigated the ontogeny of light-driven responses in mouse retinal ganglion cells (RGCs). Using a large-scale, high-density multielectrode array, we recorded from hundreds to thousands of RGCs simultaneously at pan-retinal level, including dorsal and ventral locations. Responses to different contrasts not only revealed a complex developmental profile for ON, OFF and ON-OFF responses, but also unveiled differences between dorsal and ventral RGC responses. At eye-opening, dorsal RGCs of all types were more responsive to light, perhaps indicating an environmental priority to nest viewing for pre-weaning pups. The developmental profile of ON and OFF responses exhibited antagonistic behaviour, with the strongest ON responses shortly after eye-opening, followed by an increase in the strength of OFF responses later on. Further, we found that with maturation receptive field (RF) center sizes decrease, spike-triggered averaged responses to white noise become stronger, and centers become more circular while maintaining differences between RGC types. We conclude that the maturation of retinal functionality is not spatially homogeneous, likely reflecting ecological requirements that favour earlier maturation of the dorsal retina.
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spelling pubmed-53012062017-02-13 Pan-retinal characterisation of Light Responses from Ganglion Cells in the Developing Mouse Retina Hilgen, Gerrit Pirmoradian, Sahar Pamplona, Daniela Kornprobst, Pierre Cessac, Bruno Hennig, Matthias H. Sernagor, Evelyne Sci Rep Article We have investigated the ontogeny of light-driven responses in mouse retinal ganglion cells (RGCs). Using a large-scale, high-density multielectrode array, we recorded from hundreds to thousands of RGCs simultaneously at pan-retinal level, including dorsal and ventral locations. Responses to different contrasts not only revealed a complex developmental profile for ON, OFF and ON-OFF responses, but also unveiled differences between dorsal and ventral RGC responses. At eye-opening, dorsal RGCs of all types were more responsive to light, perhaps indicating an environmental priority to nest viewing for pre-weaning pups. The developmental profile of ON and OFF responses exhibited antagonistic behaviour, with the strongest ON responses shortly after eye-opening, followed by an increase in the strength of OFF responses later on. Further, we found that with maturation receptive field (RF) center sizes decrease, spike-triggered averaged responses to white noise become stronger, and centers become more circular while maintaining differences between RGC types. We conclude that the maturation of retinal functionality is not spatially homogeneous, likely reflecting ecological requirements that favour earlier maturation of the dorsal retina. Nature Publishing Group 2017-02-10 /pmc/articles/PMC5301206/ /pubmed/28186129 http://dx.doi.org/10.1038/srep42330 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Hilgen, Gerrit
Pirmoradian, Sahar
Pamplona, Daniela
Kornprobst, Pierre
Cessac, Bruno
Hennig, Matthias H.
Sernagor, Evelyne
Pan-retinal characterisation of Light Responses from Ganglion Cells in the Developing Mouse Retina
title Pan-retinal characterisation of Light Responses from Ganglion Cells in the Developing Mouse Retina
title_full Pan-retinal characterisation of Light Responses from Ganglion Cells in the Developing Mouse Retina
title_fullStr Pan-retinal characterisation of Light Responses from Ganglion Cells in the Developing Mouse Retina
title_full_unstemmed Pan-retinal characterisation of Light Responses from Ganglion Cells in the Developing Mouse Retina
title_short Pan-retinal characterisation of Light Responses from Ganglion Cells in the Developing Mouse Retina
title_sort pan-retinal characterisation of light responses from ganglion cells in the developing mouse retina
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5301206/
https://www.ncbi.nlm.nih.gov/pubmed/28186129
http://dx.doi.org/10.1038/srep42330
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