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Label-free detection of myocardial ischaemia in the perfused rat heart by spontaneous Raman spectroscopy

Raman spectroscopy, which identifies intrinsic molecular constituents, has a potential for determining myocardial viability under label-free conditions. However, its suitability for evaluating myocardial ischaemia is undetermined. Focusing on cytochromes, i.e., representative molecules reflecting mi...

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Autores principales: Ohira, Suguru, Tanaka, Hideo, Harada, Yoshinori, Minamikawa, Takeo, Kumamoto, Yasuaki, Matoba, Satoaki, Yaku, Hitoshi, Takamatsu, Tetsuro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5301243/
https://www.ncbi.nlm.nih.gov/pubmed/28186163
http://dx.doi.org/10.1038/srep42401
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author Ohira, Suguru
Tanaka, Hideo
Harada, Yoshinori
Minamikawa, Takeo
Kumamoto, Yasuaki
Matoba, Satoaki
Yaku, Hitoshi
Takamatsu, Tetsuro
author_facet Ohira, Suguru
Tanaka, Hideo
Harada, Yoshinori
Minamikawa, Takeo
Kumamoto, Yasuaki
Matoba, Satoaki
Yaku, Hitoshi
Takamatsu, Tetsuro
author_sort Ohira, Suguru
collection PubMed
description Raman spectroscopy, which identifies intrinsic molecular constituents, has a potential for determining myocardial viability under label-free conditions. However, its suitability for evaluating myocardial ischaemia is undetermined. Focusing on cytochromes, i.e., representative molecules reflecting mitochondrial activity, we tested whether Raman spectroscopy is applicable for evaluating myocardial ischaemia especially during early ischaemic phase. We obtained spontaneous Raman spectra of the subepicardial myocardium in the Langendorff-perfused rat heart upon 532-nm excitation before and during the “stopped-flow,” global ischaemia. Semi-quantitative values of the peak intensities at 750 and 1127 cm(−1), which reflect reduced cytochromes c and b, increased immediately and progressively after induction of the stopped flow, indicating progressive reduction of the mitochondrial respiration. Such spectral changes emerged before the loss of 1) mitochondrial membrane potentials measured by the fluorescence intensity of tetramethyl rhodamine ethyl ester or 2) staining of the triphenyl tetrazolium chloride dye in the myocardium. The progressive increases in the Raman peaks by stopped flow were significantly retarded by ischaemic preconditioning. Sequential measurements of the peak intensities at 750 and 1127 cm(−1) enabled early detection of the myocardial ischaemia based on the mitochondrial functions. These data suggest that Raman spectroscopy offers the potential to evaluate acute ischaemic heart under label-free conditions.
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spelling pubmed-53012432017-02-13 Label-free detection of myocardial ischaemia in the perfused rat heart by spontaneous Raman spectroscopy Ohira, Suguru Tanaka, Hideo Harada, Yoshinori Minamikawa, Takeo Kumamoto, Yasuaki Matoba, Satoaki Yaku, Hitoshi Takamatsu, Tetsuro Sci Rep Article Raman spectroscopy, which identifies intrinsic molecular constituents, has a potential for determining myocardial viability under label-free conditions. However, its suitability for evaluating myocardial ischaemia is undetermined. Focusing on cytochromes, i.e., representative molecules reflecting mitochondrial activity, we tested whether Raman spectroscopy is applicable for evaluating myocardial ischaemia especially during early ischaemic phase. We obtained spontaneous Raman spectra of the subepicardial myocardium in the Langendorff-perfused rat heart upon 532-nm excitation before and during the “stopped-flow,” global ischaemia. Semi-quantitative values of the peak intensities at 750 and 1127 cm(−1), which reflect reduced cytochromes c and b, increased immediately and progressively after induction of the stopped flow, indicating progressive reduction of the mitochondrial respiration. Such spectral changes emerged before the loss of 1) mitochondrial membrane potentials measured by the fluorescence intensity of tetramethyl rhodamine ethyl ester or 2) staining of the triphenyl tetrazolium chloride dye in the myocardium. The progressive increases in the Raman peaks by stopped flow were significantly retarded by ischaemic preconditioning. Sequential measurements of the peak intensities at 750 and 1127 cm(−1) enabled early detection of the myocardial ischaemia based on the mitochondrial functions. These data suggest that Raman spectroscopy offers the potential to evaluate acute ischaemic heart under label-free conditions. Nature Publishing Group 2017-02-10 /pmc/articles/PMC5301243/ /pubmed/28186163 http://dx.doi.org/10.1038/srep42401 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Ohira, Suguru
Tanaka, Hideo
Harada, Yoshinori
Minamikawa, Takeo
Kumamoto, Yasuaki
Matoba, Satoaki
Yaku, Hitoshi
Takamatsu, Tetsuro
Label-free detection of myocardial ischaemia in the perfused rat heart by spontaneous Raman spectroscopy
title Label-free detection of myocardial ischaemia in the perfused rat heart by spontaneous Raman spectroscopy
title_full Label-free detection of myocardial ischaemia in the perfused rat heart by spontaneous Raman spectroscopy
title_fullStr Label-free detection of myocardial ischaemia in the perfused rat heart by spontaneous Raman spectroscopy
title_full_unstemmed Label-free detection of myocardial ischaemia in the perfused rat heart by spontaneous Raman spectroscopy
title_short Label-free detection of myocardial ischaemia in the perfused rat heart by spontaneous Raman spectroscopy
title_sort label-free detection of myocardial ischaemia in the perfused rat heart by spontaneous raman spectroscopy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5301243/
https://www.ncbi.nlm.nih.gov/pubmed/28186163
http://dx.doi.org/10.1038/srep42401
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