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The Bone Regeneration Using Bone Marrow Stromal Cells with Moderate Concentration Platelet-Rich Plasma in Femoral Segmental Defect of Rats

BACKGROUND: Platelet-rich plasma (PRP) can provide an assortment of growth factors, but how PRP effects bone regeneration is still unknown. The aim of the study was to explore an optimal method of using PRP and bone marrow stromal cells (BMSCs). METHODS: An in vitro experiment was first conducted to...

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Detalles Bibliográficos
Autores principales: Yamakawa, Junichi, Hashimoto, Junichi, Takano, Mitsuo, Takagi, Michiaki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Bentham Open 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5301304/
https://www.ncbi.nlm.nih.gov/pubmed/28217215
http://dx.doi.org/10.2174/1874325001711010001
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author Yamakawa, Junichi
Hashimoto, Junichi
Takano, Mitsuo
Takagi, Michiaki
author_facet Yamakawa, Junichi
Hashimoto, Junichi
Takano, Mitsuo
Takagi, Michiaki
author_sort Yamakawa, Junichi
collection PubMed
description BACKGROUND: Platelet-rich plasma (PRP) can provide an assortment of growth factors, but how PRP effects bone regeneration is still unknown. The aim of the study was to explore an optimal method of using PRP and bone marrow stromal cells (BMSCs). METHODS: An in vitro experiment was first conducted to determine an appropriate quantity of PRP. BMSCs were cultured with PRP of different concentrations to assess cell proliferation and osteogenic differentiation. Following the in vitro study, a rat femoral segmental defect model was used. Five collagen mixtures consisting of different concentrations of PRP and BMSCs were prepared as follows, i) BMSCs and PRP (platelet 20 x 10(4)/µl), ii) BMSCs and PRP (platelet 100 x 10(4)/µl), iii) BMSCs and PRP (platelet 500 x 10(4)/µl), iv) BMSCs, and v) PRP group (platelet 100 x 10(4)/µl), were used to fill defect. New bone formation was evaluated by soft X-ray and histologic analyses were performed at 2, 4, 6 and 8 weeks postoperatively. RESULTS: The cell proliferation increased PRP concentration-dependently. Cellular alkaline phosphatase activity was higher in moderate concentration than high or low concentration group’s in vitro study. In vivo study, the bone fill percentage of newly formed bone in BMSCs and PRP (platelet 100 x 10(4)/µl) was 46.9% at 8 weeks and increased significantly compared with other groups. CONCLUSION: BMSCs with moderate level of PRP significantly enhanced bone formation in comparison with BMSCs or PRP transplant in a rat femoral defect model.
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spelling pubmed-53013042017-02-17 The Bone Regeneration Using Bone Marrow Stromal Cells with Moderate Concentration Platelet-Rich Plasma in Femoral Segmental Defect of Rats Yamakawa, Junichi Hashimoto, Junichi Takano, Mitsuo Takagi, Michiaki Open Orthop J Article BACKGROUND: Platelet-rich plasma (PRP) can provide an assortment of growth factors, but how PRP effects bone regeneration is still unknown. The aim of the study was to explore an optimal method of using PRP and bone marrow stromal cells (BMSCs). METHODS: An in vitro experiment was first conducted to determine an appropriate quantity of PRP. BMSCs were cultured with PRP of different concentrations to assess cell proliferation and osteogenic differentiation. Following the in vitro study, a rat femoral segmental defect model was used. Five collagen mixtures consisting of different concentrations of PRP and BMSCs were prepared as follows, i) BMSCs and PRP (platelet 20 x 10(4)/µl), ii) BMSCs and PRP (platelet 100 x 10(4)/µl), iii) BMSCs and PRP (platelet 500 x 10(4)/µl), iv) BMSCs, and v) PRP group (platelet 100 x 10(4)/µl), were used to fill defect. New bone formation was evaluated by soft X-ray and histologic analyses were performed at 2, 4, 6 and 8 weeks postoperatively. RESULTS: The cell proliferation increased PRP concentration-dependently. Cellular alkaline phosphatase activity was higher in moderate concentration than high or low concentration group’s in vitro study. In vivo study, the bone fill percentage of newly formed bone in BMSCs and PRP (platelet 100 x 10(4)/µl) was 46.9% at 8 weeks and increased significantly compared with other groups. CONCLUSION: BMSCs with moderate level of PRP significantly enhanced bone formation in comparison with BMSCs or PRP transplant in a rat femoral defect model. Bentham Open 2017-01-30 /pmc/articles/PMC5301304/ /pubmed/28217215 http://dx.doi.org/10.2174/1874325001711010001 Text en © Yamakawa et al.; Licensee Bentham Open https://creativecommons.org/licenses/by-nc/4.0/legalcode This is an open access article licensed under the terms of the Creative Commons Attribution-Non-Commercial 4.0 International Public License (CC BY-NC 4.0) (https://creativecommons.org/licenses/by-nc/4.0/legalcode), which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
spellingShingle Article
Yamakawa, Junichi
Hashimoto, Junichi
Takano, Mitsuo
Takagi, Michiaki
The Bone Regeneration Using Bone Marrow Stromal Cells with Moderate Concentration Platelet-Rich Plasma in Femoral Segmental Defect of Rats
title The Bone Regeneration Using Bone Marrow Stromal Cells with Moderate Concentration Platelet-Rich Plasma in Femoral Segmental Defect of Rats
title_full The Bone Regeneration Using Bone Marrow Stromal Cells with Moderate Concentration Platelet-Rich Plasma in Femoral Segmental Defect of Rats
title_fullStr The Bone Regeneration Using Bone Marrow Stromal Cells with Moderate Concentration Platelet-Rich Plasma in Femoral Segmental Defect of Rats
title_full_unstemmed The Bone Regeneration Using Bone Marrow Stromal Cells with Moderate Concentration Platelet-Rich Plasma in Femoral Segmental Defect of Rats
title_short The Bone Regeneration Using Bone Marrow Stromal Cells with Moderate Concentration Platelet-Rich Plasma in Femoral Segmental Defect of Rats
title_sort bone regeneration using bone marrow stromal cells with moderate concentration platelet-rich plasma in femoral segmental defect of rats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5301304/
https://www.ncbi.nlm.nih.gov/pubmed/28217215
http://dx.doi.org/10.2174/1874325001711010001
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