Expression of mammalian mitochondrial F(1)‐ATPase in Escherichia coli depends on two chaperone factors, AF1 and AF2

F(1)‐ATPase (F(1)) is a multisubunit water‐soluble domain of F(o)F(1‐) ATP synthase and is a rotary enzyme by itself. Earlier genetic studies using yeast suggested that two factors, Atp11p and Atp12p, contribute to F(1) assembly. Here, we show that their mammalian counterparts, AF1 and AF2, are esse...

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Autores principales: Suzuki, Toshiharu, Iida, Naoya, Suzuki, Junko, Watanabe, Yasunori, Endo, Toshiya, Hisabori, Toru, Yoshida, Masasuke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2016
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5302055/
https://www.ncbi.nlm.nih.gov/pubmed/28203526
http://dx.doi.org/10.1002/2211-5463.12143
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author Suzuki, Toshiharu
Iida, Naoya
Suzuki, Junko
Watanabe, Yasunori
Endo, Toshiya
Hisabori, Toru
Yoshida, Masasuke
author_facet Suzuki, Toshiharu
Iida, Naoya
Suzuki, Junko
Watanabe, Yasunori
Endo, Toshiya
Hisabori, Toru
Yoshida, Masasuke
author_sort Suzuki, Toshiharu
collection PubMed
description F(1)‐ATPase (F(1)) is a multisubunit water‐soluble domain of F(o)F(1‐) ATP synthase and is a rotary enzyme by itself. Earlier genetic studies using yeast suggested that two factors, Atp11p and Atp12p, contribute to F(1) assembly. Here, we show that their mammalian counterparts, AF1 and AF2, are essential and sufficient for efficient production of recombinant bovine mitochondrial F(1) in Escherichia coli cells. Intactness of the function and conformation of the E. coli‐expressed bovine F(1) was verified by rotation analysis and crystallization. This expression system opens a way for the previously unattempted mutation study of mammalian mitochondrial F(1).
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spelling pubmed-53020552017-02-15 Expression of mammalian mitochondrial F(1)‐ATPase in Escherichia coli depends on two chaperone factors, AF1 and AF2 Suzuki, Toshiharu Iida, Naoya Suzuki, Junko Watanabe, Yasunori Endo, Toshiya Hisabori, Toru Yoshida, Masasuke FEBS Open Bio Research Articles F(1)‐ATPase (F(1)) is a multisubunit water‐soluble domain of F(o)F(1‐) ATP synthase and is a rotary enzyme by itself. Earlier genetic studies using yeast suggested that two factors, Atp11p and Atp12p, contribute to F(1) assembly. Here, we show that their mammalian counterparts, AF1 and AF2, are essential and sufficient for efficient production of recombinant bovine mitochondrial F(1) in Escherichia coli cells. Intactness of the function and conformation of the E. coli‐expressed bovine F(1) was verified by rotation analysis and crystallization. This expression system opens a way for the previously unattempted mutation study of mammalian mitochondrial F(1). John Wiley and Sons Inc. 2016-10-25 /pmc/articles/PMC5302055/ /pubmed/28203526 http://dx.doi.org/10.1002/2211-5463.12143 Text en © 2016 The Authors. Published by FEBS Press and John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Suzuki, Toshiharu
Iida, Naoya
Suzuki, Junko
Watanabe, Yasunori
Endo, Toshiya
Hisabori, Toru
Yoshida, Masasuke
Expression of mammalian mitochondrial F(1)‐ATPase in Escherichia coli depends on two chaperone factors, AF1 and AF2
title Expression of mammalian mitochondrial F(1)‐ATPase in Escherichia coli depends on two chaperone factors, AF1 and AF2
title_full Expression of mammalian mitochondrial F(1)‐ATPase in Escherichia coli depends on two chaperone factors, AF1 and AF2
title_fullStr Expression of mammalian mitochondrial F(1)‐ATPase in Escherichia coli depends on two chaperone factors, AF1 and AF2
title_full_unstemmed Expression of mammalian mitochondrial F(1)‐ATPase in Escherichia coli depends on two chaperone factors, AF1 and AF2
title_short Expression of mammalian mitochondrial F(1)‐ATPase in Escherichia coli depends on two chaperone factors, AF1 and AF2
title_sort expression of mammalian mitochondrial f(1)‐atpase in escherichia coli depends on two chaperone factors, af1 and af2
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5302055/
https://www.ncbi.nlm.nih.gov/pubmed/28203526
http://dx.doi.org/10.1002/2211-5463.12143
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