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Eukaryotic translation initiation factor 3 subunit D overexpression is associated with the occurrence and development of ovarian cancer
Ovarian cancer is the most common cause of gynaecological cancer‐associated death; thus, promising biomarkers and new therapeutic targets for ovarian cancer must be explored. Here, we report that eukaryotic translation initiation factor 3 subunit D (EIF3D), a member of the EIF3 family, was overexpre...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5302064/ https://www.ncbi.nlm.nih.gov/pubmed/28203520 http://dx.doi.org/10.1002/2211-5463.12137 |
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author | Lin, Yaying Zhang, Rongrong Zhang, Ping |
author_facet | Lin, Yaying Zhang, Rongrong Zhang, Ping |
author_sort | Lin, Yaying |
collection | PubMed |
description | Ovarian cancer is the most common cause of gynaecological cancer‐associated death; thus, promising biomarkers and new therapeutic targets for ovarian cancer must be explored. Here, we report that eukaryotic translation initiation factor 3 subunit D (EIF3D), a member of the EIF3 family, was overexpressed in ovarian cancer clinical tissues. Furthermore, the expression of EIF3D was correlated with the International Federation of Gynecology and Obstetrics stage and pathological differentiation stage. 3‐(4,5‐dimethylthylthiazol‐2‐yl)‐2 (MTT) and colony formation assays revealed that the lentivirus‐mediated knockdown of EIF3D suppresses cell proliferation in the ovarian tumour cell lines CAOV‐3 and SKOV‐3. Flow cytometry revealed that cells were arrested at the G2/M phase of the cell cycle and that cyclin‐dependent kinase 1 was also altered after EIF3D silencing. The results presented here demonstrate that EIF3D may play an important role in the occurrence and development of ovarian cancer. |
format | Online Article Text |
id | pubmed-5302064 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-53020642017-02-15 Eukaryotic translation initiation factor 3 subunit D overexpression is associated with the occurrence and development of ovarian cancer Lin, Yaying Zhang, Rongrong Zhang, Ping FEBS Open Bio Research Articles Ovarian cancer is the most common cause of gynaecological cancer‐associated death; thus, promising biomarkers and new therapeutic targets for ovarian cancer must be explored. Here, we report that eukaryotic translation initiation factor 3 subunit D (EIF3D), a member of the EIF3 family, was overexpressed in ovarian cancer clinical tissues. Furthermore, the expression of EIF3D was correlated with the International Federation of Gynecology and Obstetrics stage and pathological differentiation stage. 3‐(4,5‐dimethylthylthiazol‐2‐yl)‐2 (MTT) and colony formation assays revealed that the lentivirus‐mediated knockdown of EIF3D suppresses cell proliferation in the ovarian tumour cell lines CAOV‐3 and SKOV‐3. Flow cytometry revealed that cells were arrested at the G2/M phase of the cell cycle and that cyclin‐dependent kinase 1 was also altered after EIF3D silencing. The results presented here demonstrate that EIF3D may play an important role in the occurrence and development of ovarian cancer. John Wiley and Sons Inc. 2016-10-31 /pmc/articles/PMC5302064/ /pubmed/28203520 http://dx.doi.org/10.1002/2211-5463.12137 Text en © 2016 The Authors. Published by FEBS Press and John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Lin, Yaying Zhang, Rongrong Zhang, Ping Eukaryotic translation initiation factor 3 subunit D overexpression is associated with the occurrence and development of ovarian cancer |
title | Eukaryotic translation initiation factor 3 subunit D overexpression is associated with the occurrence and development of ovarian cancer |
title_full | Eukaryotic translation initiation factor 3 subunit D overexpression is associated with the occurrence and development of ovarian cancer |
title_fullStr | Eukaryotic translation initiation factor 3 subunit D overexpression is associated with the occurrence and development of ovarian cancer |
title_full_unstemmed | Eukaryotic translation initiation factor 3 subunit D overexpression is associated with the occurrence and development of ovarian cancer |
title_short | Eukaryotic translation initiation factor 3 subunit D overexpression is associated with the occurrence and development of ovarian cancer |
title_sort | eukaryotic translation initiation factor 3 subunit d overexpression is associated with the occurrence and development of ovarian cancer |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5302064/ https://www.ncbi.nlm.nih.gov/pubmed/28203520 http://dx.doi.org/10.1002/2211-5463.12137 |
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