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Leptin pharmacokinetics in male mice

Pharmacokinetics of leptin in mammals has not been studied in detail and only one study has examined more than one time point in non-mutant mice and this was in a female mice. This is the first study to describe leptin distribution over a detailed time course in normal male mice. A physiologic dose...

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Autores principales: Hart, Robert A, Dobos, Robin C, Agnew, Linda L, Smart, Neil A, McFarlane, James R
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Bioscientifica Ltd 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5302164/
https://www.ncbi.nlm.nih.gov/pubmed/27998953
http://dx.doi.org/10.1530/EC-16-0089
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author Hart, Robert A
Dobos, Robin C
Agnew, Linda L
Smart, Neil A
McFarlane, James R
author_facet Hart, Robert A
Dobos, Robin C
Agnew, Linda L
Smart, Neil A
McFarlane, James R
author_sort Hart, Robert A
collection PubMed
description Pharmacokinetics of leptin in mammals has not been studied in detail and only one study has examined more than one time point in non-mutant mice and this was in a female mice. This is the first study to describe leptin distribution over a detailed time course in normal male mice. A physiologic dose (12 ng) of radiolabelled leptin was injected into adult male mice via the lateral tail vein and tissues were dissected out and measured for radioactivity over a time course of up to two hours. Major targets were the digestive tract, kidneys, skin and lungs. The brain was not a major target, and 0.15% of the total dose was recovered from the brain 5 min after administration. Major differences appear to exist in the distribution of leptin between the male and female mice, indicating a high degree of sexual dimorphism. Although the half-lives were similar between male and female mice, almost twice the proportion of leptin was recovered from the digestive tract of male mice in comparison to that reported previously for females. This would seem to indicate a major difference in leptin distribution and possibly function between males and females.
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spelling pubmed-53021642017-03-06 Leptin pharmacokinetics in male mice Hart, Robert A Dobos, Robin C Agnew, Linda L Smart, Neil A McFarlane, James R Endocr Connect Research Pharmacokinetics of leptin in mammals has not been studied in detail and only one study has examined more than one time point in non-mutant mice and this was in a female mice. This is the first study to describe leptin distribution over a detailed time course in normal male mice. A physiologic dose (12 ng) of radiolabelled leptin was injected into adult male mice via the lateral tail vein and tissues were dissected out and measured for radioactivity over a time course of up to two hours. Major targets were the digestive tract, kidneys, skin and lungs. The brain was not a major target, and 0.15% of the total dose was recovered from the brain 5 min after administration. Major differences appear to exist in the distribution of leptin between the male and female mice, indicating a high degree of sexual dimorphism. Although the half-lives were similar between male and female mice, almost twice the proportion of leptin was recovered from the digestive tract of male mice in comparison to that reported previously for females. This would seem to indicate a major difference in leptin distribution and possibly function between males and females. Bioscientifica Ltd 2016-12-20 /pmc/articles/PMC5302164/ /pubmed/27998953 http://dx.doi.org/10.1530/EC-16-0089 Text en © 2017 The authors http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research
Hart, Robert A
Dobos, Robin C
Agnew, Linda L
Smart, Neil A
McFarlane, James R
Leptin pharmacokinetics in male mice
title Leptin pharmacokinetics in male mice
title_full Leptin pharmacokinetics in male mice
title_fullStr Leptin pharmacokinetics in male mice
title_full_unstemmed Leptin pharmacokinetics in male mice
title_short Leptin pharmacokinetics in male mice
title_sort leptin pharmacokinetics in male mice
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5302164/
https://www.ncbi.nlm.nih.gov/pubmed/27998953
http://dx.doi.org/10.1530/EC-16-0089
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